SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000747
Component protein type	TssL
T6SS ID (Type)	T6SS00084 (Type i1)
Strain	Aliivibrio fischeri ES114
Replicon	chromosome I
Sequence	Protein sequence (278 a.a.); Nucleotide sequence (834 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VF_RS05190
Coordinate (Strand)	1096135..1096968 (-)
NCBI ID	WP_005418702.1
RefSeq	NC_006840
Uniprot ID	Q5E657_ALIF1, B5FD79_ALIFM, A0A1B9PCV3_ALIFS
KEGG ID	vfm:VFMJ11_1076, vfi:VF_0994
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.6e-62	58..259

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-238	MNSSSMDDRTVLITQEPNQVNPTLTESPSAPKFNQLQERMVYAARIKPEEYNFAGNNPLISAASSLLCITCDLKKESYQDIELLKPKLVEELKSFEFQAINSGVESNYVALSRYLLCTFVDEIIVTTPWGSESHWASKSMLSSFHNETYGGEKFFLLLEKLSRNPAKYLDLLELMYLCLSLGFEGKYRVLSRGLVELDAIRDSLYRQIRSLRGDEQASLSPNWEPKQVQRHKLTRQTS
Transmembrane helix	239-261	WVGIVCVVVFSLCAIYGGFYYVL
Outside	262-277	DQQSEHVIQAFQQIDL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9700	-	-

Protein sequence: 278 a.a.

						>T6CP000747 NC_006840:c1096968-1096135 [Aliivibrio fischeri ES114] [TssL]
MNSSSMDDRTVLITQEPNQVNPTLTESPSAPKFNQLQERMVYAARIKPEEYNFAGNNPLISAASSLLCITCDLKKESYQD
IELLKPKLVEELKSFEFQAINSGVESNYVALSRYLLCTFVDEIIVTTPWGSESHWASKSMLSSFHNETYGGEKFFLLLEK
LSRNPAKYLDLLELMYLCLSLGFEGKYRVLSRGLVELDAIRDSLYRQIRSLRGDEQASLSPNWEPKQVQRHKLTRQTSWV
GIVCVVVFSLCAIYGGFYYVLDQQSEHVIQAFQQIDL*					

Nucleotide sequence: 834 bp

						>T6CP000747 NC_006840:c1096968-1096135 [Aliivibrio fischeri ES114] [TssL]
ATGAACAGCAGCAGCATGGATGACCGTACTGTACTTATCACACAAGAGCCTAATCAAGTTAACCCAACTTTAACTGAAAG
CCCAAGTGCACCCAAATTCAACCAGCTTCAAGAGCGCATGGTCTACGCCGCGCGTATTAAACCAGAAGAATACAATTTTG
CAGGAAACAACCCACTAATCAGTGCTGCTTCCTCATTACTCTGTATCACGTGTGACTTAAAAAAAGAAAGCTACCAAGAT
ATTGAGTTACTAAAACCTAAATTAGTTGAAGAGTTAAAATCCTTCGAGTTTCAAGCCATTAACTCAGGTGTTGAGAGTAA
TTATGTTGCCTTATCTCGCTACCTGCTTTGCACTTTCGTTGATGAAATCATCGTAACAACGCCATGGGGTTCAGAAAGTC
ACTGGGCCTCAAAAAGCATGTTAAGTAGCTTTCATAACGAAACATATGGTGGTGAGAAATTTTTCTTACTATTAGAAAAA
CTCAGTAGAAACCCTGCTAAGTATTTAGATCTATTAGAGCTAATGTACCTATGTTTGAGCTTAGGTTTTGAAGGTAAATA
TCGAGTGTTATCAAGAGGTCTAGTCGAATTAGACGCCATTCGTGATTCTCTTTATCGCCAAATTCGCTCTTTACGAGGAG
ATGAGCAAGCTAGCCTCTCTCCAAACTGGGAACCGAAACAAGTTCAACGCCATAAACTCACAAGACAAACCTCTTGGGTT
GGCATTGTATGCGTTGTGGTTTTCTCATTGTGTGCTATTTACGGTGGTTTCTATTATGTATTGGACCAACAATCAGAGCA
TGTAATTCAAGCTTTTCAGCAAATTGATTTATAG					