SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000746
Component protein type	TssM
T6SS ID (Type)	T6SS00084 (Type i1)
Strain	Aliivibrio fischeri ES114
Replicon	chromosome I
Sequence	Protein sequence (1174 a.a.); Nucleotide sequence (3522 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Guckes KR, Cecere AG, Wasilko NP, et al. Incompatibility of Vibrio fischeri Strains during Symbiosis Establishment Depends on Two Functionally Redundant hcp Genes. J Bacteriol. 2019 Sep 6;201(19). pii: e00221-19. doi: 10.1128/JB.00221-19. Print 2019 Oct 1. PMID: 31331977

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	VF_RS05185
Coordinate (Strand)	1092603..1096124 (-)
NCBI ID	WP_011261627.1
RefSeq	NC_006840
Uniprot ID	Q5E658_ALIF1
KEGG ID	vfi:VF_0993
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related_N	PF14331.8	6.2e-74	203..452
IcmF-related	PF06761.14	4.4e-67	502..795
IcmF_C	PF06744.14	3.9e-19	1046..1150

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-16	MKSFFNSLLNTLKKTW
Transmembrane helix	17-39	CWSLLLTLFIALLLCLFGKHIAI
Outside	40-53	ANTPLIETTTGKTL
Transmembrane helix	54-73	TIIICLLLWGAFNLTLWGLA
Inside	74-446	KREAQKEENKEAYEAAQSEMAFIREHSTILKTKLEAAIATIKRAGLYGKLNNNVKYQLPWYMVIGPQNSGKTTLLECSGLDFPLNQTDGHYTRDIQNSQHCEWYFANHAVLLDAAGRFFDQHENELSKPIWGNFLKALRNKRRRRPLNGVILTIDITTLQSPDESAIEVQARYVRERLQELRHDLSSDMPIYFLLTKMDKVEGFEPFFSSLSREEMDQVFGVTFNEGEGQQADKIKHEFEKLILRIDSQVMSRLHDERDVMKRAELLQFPRQLSFLAERLALFSELAFTKTRYHQASHLRGLYLTSVPEPTNTQTESTTSGIGQNLGISSRVLPTYKHQRGFFIRKLLEDVIFPNSELATLDEKYEKQVKLKN
Transmembrane helix	447-469	MAVYAASFALVIGFGSLWATSFL
Outside	470-1173	TNHHKQQELLSLSGDYNQQIVHLSPLAPSIELLPTLNTLLAATQVYSKESETLTDEFAGLQQGDKLRKAAEAAYHRSLLSLLLPKVTSELEYQVANNQDNREFLTPSLRAYLMLNLHEHLDKAYLEQWMGLHWSYLYSGSATEQKELQAHFSNLLNIEFEPVQLNDNLIAKSRKFLREADTSELVYQQLKQDAKEMDLRDIKISDHLGPNQNLFNHTNTIIPGLYTQKGYKAVFLSKGLDQVKQLIEENWVIGLSSDLSANEIRGLYADVEDLYFHDYIKYWKEAVDQLQIKPSKNMDEAILQISNITGSSQPILKLLNLVKENTTFVDKAQVASKTAGQVKKLPVNTKLKKVASLGLHSVEERSQSARKAVSNQFESLNLLLTDKDQATIPLEDALVVINELSGRLSMVKFSPNPDSSAFKIARDRMQGIPNELNDIRVMAETLPQPLQQWWKQISNNAWGIILNHSRKHVQIVYHDKVVTHYDVALSGRYPFRNSSHDVNIADFDEFFQNGGILDNFFDAYLSSFVYKQKGKFKQKSLHGRSLGLSDSFLDQYNYGLTIQRMFYGSKGKEANVSFRIAPFELDASALQSTFYYGKNKFAYRHGPIQKKQFTWPITNQRQYVSFVLQDLSGSKVVNQQNSGPWAFFRVLDKFRVNKYRGQDVIKVDLENKGMKAKYLIYSDRTPNPFNRKILTNFYLPKRING

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9779	-	-

Protein sequence: 1174 a.a.

						>T6CP000746 NC_006840:c1096124-1092603 [Aliivibrio fischeri ES114] [TssM]
MKSFFNSLLNTLKKTWCWSLLLTLFIALLLCLFGKHIAIANTPLIETTTGKTLTIIICLLLWGAFNLTLWGLAKREAQKE
ENKEAYEAAQSEMAFIREHSTILKTKLEAAIATIKRAGLYGKLNNNVKYQLPWYMVIGPQNSGKTTLLECSGLDFPLNQT
DGHYTRDIQNSQHCEWYFANHAVLLDAAGRFFDQHENELSKPIWGNFLKALRNKRRRRPLNGVILTIDITTLQSPDESAI
EVQARYVRERLQELRHDLSSDMPIYFLLTKMDKVEGFEPFFSSLSREEMDQVFGVTFNEGEGQQADKIKHEFEKLILRID
SQVMSRLHDERDVMKRAELLQFPRQLSFLAERLALFSELAFTKTRYHQASHLRGLYLTSVPEPTNTQTESTTSGIGQNLG
ISSRVLPTYKHQRGFFIRKLLEDVIFPNSELATLDEKYEKQVKLKNMAVYAASFALVIGFGSLWATSFLTNHHKQQELLS
LSGDYNQQIVHLSPLAPSIELLPTLNTLLAATQVYSKESETLTDEFAGLQQGDKLRKAAEAAYHRSLLSLLLPKVTSELE
YQVANNQDNREFLTPSLRAYLMLNLHEHLDKAYLEQWMGLHWSYLYSGSATEQKELQAHFSNLLNIEFEPVQLNDNLIAK
SRKFLREADTSELVYQQLKQDAKEMDLRDIKISDHLGPNQNLFNHTNTIIPGLYTQKGYKAVFLSKGLDQVKQLIEENWV
IGLSSDLSANEIRGLYADVEDLYFHDYIKYWKEAVDQLQIKPSKNMDEAILQISNITGSSQPILKLLNLVKENTTFVDKA
QVASKTAGQVKKLPVNTKLKKVASLGLHSVEERSQSARKAVSNQFESLNLLLTDKDQATIPLEDALVVINELSGRLSMVK
FSPNPDSSAFKIARDRMQGIPNELNDIRVMAETLPQPLQQWWKQISNNAWGIILNHSRKHVQIVYHDKVVTHYDVALSGR
YPFRNSSHDVNIADFDEFFQNGGILDNFFDAYLSSFVYKQKGKFKQKSLHGRSLGLSDSFLDQYNYGLTIQRMFYGSKGK
EANVSFRIAPFELDASALQSTFYYGKNKFAYRHGPIQKKQFTWPITNQRQYVSFVLQDLSGSKVVNQQNSGPWAFFRVLD
KFRVNKYRGQDVIKVDLENKGMKAKYLIYSDRTPNPFNRKILTNFYLPKRING*					

Nucleotide sequence: 3522 bp

						>T6CP000746 NC_006840:c1096124-1092603 [Aliivibrio fischeri ES114] [TssM]
ATGAAATCATTTTTTAATTCATTGCTTAACACATTAAAAAAGACATGGTGTTGGAGCTTATTATTAACACTATTTATTGC
CTTATTATTGTGTTTATTTGGTAAGCATATTGCGATAGCAAATACCCCATTAATTGAAACGACTACCGGGAAAACATTAA
CAATTATCATTTGCTTACTACTATGGGGAGCATTCAATCTGACTTTGTGGGGCTTAGCAAAGCGAGAAGCACAAAAAGAA
GAAAATAAAGAAGCGTATGAAGCTGCGCAATCTGAAATGGCTTTTATTCGTGAACACAGCACCATTCTAAAAACAAAACT
TGAAGCTGCAATTGCAACAATCAAGCGTGCTGGGCTATATGGAAAACTCAATAATAACGTTAAATATCAACTTCCTTGGT
ATATGGTTATTGGCCCACAAAATAGTGGTAAAACAACCCTTTTAGAGTGTTCAGGATTAGATTTCCCTTTAAACCAAACT
GATGGGCATTACACAAGAGACATTCAAAATAGCCAACATTGTGAATGGTATTTTGCTAACCATGCCGTTCTTTTAGATGC
TGCAGGACGCTTTTTTGATCAACATGAAAATGAGTTAAGTAAACCAATTTGGGGCAATTTTTTAAAAGCTCTACGCAATA
AGCGTCGCCGTCGCCCATTAAATGGGGTCATACTAACTATTGATATCACAACATTACAATCTCCAGATGAAAGCGCAATC
GAAGTTCAAGCTCGCTATGTACGTGAACGCTTACAAGAGTTAAGACATGATTTAAGCTCAGATATGCCTATCTATTTCTT
ATTAACGAAAATGGATAAAGTCGAGGGATTCGAACCCTTCTTCTCTTCCCTATCCAGAGAAGAAATGGACCAAGTTTTTG
GCGTCACTTTTAACGAAGGTGAAGGCCAGCAAGCCGATAAAATTAAACATGAGTTCGAAAAGCTCATTTTACGTATCGAT
TCACAAGTCATGTCACGCTTACACGATGAGCGTGATGTGATGAAACGTGCAGAACTATTACAATTCCCACGCCAATTAAG
CTTCCTTGCTGAACGCTTAGCGTTATTTTCTGAATTAGCCTTTACTAAAACCCGTTATCACCAAGCCTCTCATTTACGTG
GTTTATACCTAACGTCAGTACCCGAACCAACAAATACCCAGACTGAATCAACCACGTCAGGTATTGGGCAAAACCTTGGT
ATCAGCTCTCGAGTTCTTCCTACTTATAAACATCAACGTGGGTTCTTTATTCGAAAATTACTTGAAGACGTGATTTTTCC
AAACAGTGAACTCGCTACATTAGATGAAAAATATGAGAAACAAGTAAAACTGAAAAACATGGCGGTCTATGCAGCCTCTT
TTGCTCTTGTGATTGGGTTTGGTTCATTATGGGCAACCAGCTTTTTAACCAATCATCATAAACAACAAGAGTTATTGTCT
CTTTCTGGCGATTATAATCAACAGATTGTCCATTTATCCCCTTTAGCTCCTTCTATTGAATTACTGCCAACATTAAATAC
TTTATTAGCAGCAACTCAAGTGTATTCAAAAGAGAGTGAAACCCTCACAGATGAGTTTGCGGGACTCCAACAAGGAGATA
AATTACGAAAGGCAGCAGAAGCCGCATACCACAGAAGTTTACTCAGTTTACTGCTTCCTAAAGTAACAAGTGAACTCGAA
TACCAAGTGGCAAATAATCAAGATAATAGAGAATTCTTAACGCCTTCTTTACGAGCATATTTGATGCTAAATCTACATGA
GCACCTTGATAAAGCTTATCTAGAACAATGGATGGGATTGCATTGGTCGTATTTATACAGTGGTTCAGCAACAGAGCAGA
AAGAGCTTCAAGCGCATTTTTCTAATCTATTAAATATCGAATTTGAACCAGTTCAATTAAACGATAATTTAATCGCTAAA
TCTCGTAAATTTTTACGTGAAGCAGATACATCAGAATTGGTATACCAACAGCTAAAACAAGACGCAAAAGAGATGGATCT
GCGCGATATAAAAATCAGTGACCATTTAGGACCAAACCAAAACTTATTCAACCACACCAATACCATTATTCCTGGTTTAT
ATACGCAAAAAGGATATAAAGCTGTCTTTTTAAGCAAAGGATTAGACCAAGTAAAACAACTGATTGAAGAAAACTGGGTC
ATTGGGTTATCAAGTGATTTAAGTGCTAATGAGATCCGTGGACTATACGCAGATGTCGAAGACCTCTATTTCCATGATTA
CATTAAATATTGGAAAGAAGCGGTAGATCAACTTCAAATTAAACCATCTAAAAACATGGATGAAGCCATTCTTCAAATTT
CAAATATTACCGGTTCAAGCCAACCAATTTTAAAGCTTTTAAACCTAGTAAAAGAAAACACCACCTTTGTTGATAAGGCT
CAAGTGGCTTCAAAAACTGCAGGTCAGGTTAAGAAACTACCTGTAAATACGAAACTGAAGAAAGTCGCCTCTCTGGGCTT
ACACAGTGTCGAAGAACGCTCTCAAAGCGCTCGTAAAGCGGTAAGCAATCAATTTGAATCACTGAACCTATTACTCACAG
ATAAAGATCAAGCAACGATTCCATTAGAAGATGCGCTGGTTGTGATCAACGAACTGAGTGGACGATTATCTATGGTGAAG
TTCTCACCTAACCCTGATTCATCAGCCTTTAAGATTGCTCGCGATCGCATGCAAGGAATACCAAATGAGTTAAATGATAT
ACGTGTAATGGCAGAAACGCTTCCCCAACCATTACAGCAATGGTGGAAACAAATATCAAATAACGCTTGGGGTATTATAC
TGAACCATTCAAGAAAACATGTACAAATAGTTTACCATGATAAAGTCGTTACTCATTACGATGTCGCTTTATCAGGTCGA
TACCCATTTAGAAACAGTAGCCATGATGTAAACATTGCTGACTTTGATGAATTCTTCCAGAACGGCGGTATTTTAGACAA
TTTCTTTGACGCCTATTTATCCTCATTTGTTTATAAACAAAAAGGGAAATTCAAACAAAAGTCCTTACACGGACGTTCAT
TAGGGCTATCTGATTCATTCTTAGACCAATATAACTATGGGTTGACTATCCAACGTATGTTTTATGGTTCAAAAGGAAAA
GAAGCCAACGTCTCGTTCCGTATTGCGCCATTTGAACTTGATGCAAGCGCTTTGCAATCCACTTTCTATTACGGAAAAAA
CAAATTTGCTTACCGTCATGGTCCAATACAGAAAAAACAATTTACGTGGCCAATAACCAATCAGCGTCAATACGTCAGTT
TTGTTTTACAAGATCTGAGTGGTTCTAAAGTCGTAAACCAACAAAACTCAGGTCCTTGGGCTTTCTTCCGAGTATTAGAC
AAATTCAGAGTCAATAAATACCGAGGTCAAGATGTCATAAAAGTAGATCTGGAAAATAAAGGAATGAAAGCCAAATACCT
TATTTATAGTGATCGAACCCCTAATCCTTTTAATAGAAAAATATTAACGAATTTCTATCTTCCAAAAAGGATTAATGGAT
GA					