SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000685
Component protein type	TssJ
T6SS ID (Type)	T6SS00076 (Type i3)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome II
Sequence	Protein sequence (173 a.a.); Nucleotide sequence (519 bp)
Reference	[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPS_RS30310
Coordinate (Strand)	2853944..2854462 (-)
NCBI ID	WP_004190712.1
RefSeq	NC_006351
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	5.5e-36	31..158

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-172	MRPRFSVSVVLGCALLLAGCGATERSVAVPYSITLDVAPDVNPDLNRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSVLGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPGEMKLQVAVKNGGIGLGGDSRVRQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9983	pos: 19-20. LAG-CG.	MRPRFSVSVVLGCALLLAG

Protein sequence: 173 a.a.

						>T6CP000685 NC_006351:c2854462-2853944 [Burkholderia pseudomallei K96243] [TssJ]
MRPRFSVSVVLGCALLLAGCGATERSVAVPYSITLDVAPDVNPDLNRKPSPIVLKVFQLKTASAFESADFFSLQDKPQSV
LGADLLGVDRIILRPGDARTLHYRGNVDAGAIGIVAEYRVLEKNRWRMTVPLPRAKQLNLYRFWQTSPGEMKLQVAVKNG
GIGLGGDSRVRQ*					

Nucleotide sequence: 519 bp

						>T6CP000685 NC_006351:c2854462-2853944 [Burkholderia pseudomallei K96243] [TssJ]
ATGCGACCGCGGTTCAGTGTTTCCGTCGTTCTGGGATGCGCGCTGCTGCTCGCCGGATGCGGGGCGACCGAGCGTTCCGT
TGCGGTGCCGTATTCGATCACGCTCGACGTCGCGCCCGACGTCAATCCGGACCTCAATCGCAAGCCGTCGCCGATCGTGC
TGAAGGTGTTCCAGCTGAAGACGGCGTCCGCGTTCGAAAGCGCCGATTTCTTCTCGCTGCAGGACAAGCCGCAGAGCGTG
CTCGGCGCGGATCTGCTCGGCGTCGACCGGATCATCCTGCGCCCGGGGGATGCGCGCACGCTGCACTATCGCGGCAACGT
CGACGCGGGCGCGATCGGCATCGTCGCCGAATATCGCGTGCTCGAGAAGAACCGCTGGCGGATGACGGTGCCGCTGCCGC
GCGCGAAGCAACTGAACCTGTACCGATTCTGGCAAACCTCGCCGGGCGAGATGAAGCTGCAGGTCGCCGTGAAGAACGGC
GGCATCGGCCTCGGCGGCGATTCGCGAGTACGCCAATGA					