Detailed information of component protein


Summary

Component ID T6CP000683
Component protein type TssL
T6SS ID (Type) T6SS00076 insolico (Type i3)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (439 a.a.); Nucleotide sequence (1317 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPS_RS30300
Coordinate (Strand) 2851212..2852528 (-)
NCBI ID WP_004190689.1
RefSeq NC_006351
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
DotU PF09850.11 1.1e-74 50..250
OmpA PF00691.22 1.5e-13 318..416
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-230 MNTSSDSFSAGSGGFVPPNPGGAHPAAAPAAGAAAFQPRPGRWAASGTNPLVAAANPLLNLVPQIRSTVHHPNPAWLREHLVVEIRQFEERAQQAGVASEAIIGARYCLCTALDEAAALTPWGGSVWSSHSLLVSFHNETWGGEKFFHLLERLSQQPRQHLDLLELLYFCLALGFEGRYRVLDNGRAQLDAVRRQLAQTIRSVRGEFDPALSPHWRDVVTRDVTRRFTVP
Transmembrane helix 231-253 LWVCVALALLVGFGVFAGLRIAL
Outside 254-438 AGHSDRLFASIDALHVPKLQPAPPAPHPAPAPRVAKFLEPEIAAGLVSVRDEADRSVIVLRGDGLFGSGSTSVIDRYMPVLTRVADALNQVQGNVRVSGYTDDTPVHTARFASNWDLSRERAQAVRSLIAARLDRPERITAEGRGTLDPVAPNDSPANRARNRRVEITLMLAPGSDAARATKEAP


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.6530 - -


  Protein sequence: 439 a.a.    

>T6CP000683 NC_006351:c2852528-2851212 [Burkholderia pseudomallei K96243] [TssL]
MNTSSDSFSAGSGGFVPPNPGGAHPAAAPAAGAAAFQPRPGRWAASGTNPLVAAANPLLNLVPQIRSTVHHPNPAWLREH
LVVEIRQFEERAQQAGVASEAIIGARYCLCTALDEAAALTPWGGSVWSSHSLLVSFHNETWGGEKFFHLLERLSQQPRQH
LDLLELLYFCLALGFEGRYRVLDNGRAQLDAVRRQLAQTIRSVRGEFDPALSPHWRDVVTRDVTRRFTVPLWVCVALALL
VGFGVFAGLRIALAGHSDRLFASIDALHVPKLQPAPPAPHPAPAPRVAKFLEPEIAAGLVSVRDEADRSVIVLRGDGLFG
SGSTSVIDRYMPVLTRVADALNQVQGNVRVSGYTDDTPVHTARFASNWDLSRERAQAVRSLIAARLDRPERITAEGRGTL
DPVAPNDSPANRARNRRVEITLMLAPGSDAARATKEAP*

  Nucleotide sequence: 1317 bp    

>T6CP000683 NC_006351:c2852528-2851212 [Burkholderia pseudomallei K96243] [TssL]
ATGAACACTTCTTCCGATTCGTTCTCGGCCGGCTCGGGCGGATTCGTGCCGCCGAATCCGGGCGGCGCGCACCCGGCCGC
CGCGCCGGCGGCGGGTGCGGCCGCGTTTCAGCCGAGGCCGGGCCGCTGGGCGGCGAGCGGCACGAATCCGCTCGTCGCGG
CCGCGAACCCGCTGCTGAACCTCGTGCCGCAGATCCGTTCGACCGTCCATCATCCGAATCCCGCGTGGCTGCGCGAGCAT
CTCGTCGTCGAGATCCGCCAGTTCGAGGAGCGCGCGCAGCAGGCGGGCGTCGCCTCCGAGGCGATCATCGGCGCGCGCTA
CTGCCTGTGCACCGCGCTCGACGAGGCCGCCGCGCTGACGCCGTGGGGCGGCAGCGTGTGGTCGTCGCATAGCCTGCTCG
TGTCGTTCCACAACGAGACGTGGGGCGGCGAGAAGTTCTTCCATCTGCTCGAGCGGCTGTCGCAGCAGCCGCGCCAGCAT
CTCGACCTGCTCGAGCTGCTGTACTTCTGCCTCGCGCTCGGCTTCGAGGGGCGCTATCGCGTGCTCGACAACGGCCGCGC
GCAGCTCGACGCGGTGCGCCGCCAGCTCGCGCAGACGATCCGCTCGGTGCGCGGCGAATTCGATCCGGCGCTCTCGCCGC
ATTGGCGCGACGTCGTCACGCGCGACGTCACGCGGCGCTTCACGGTGCCGCTGTGGGTGTGCGTCGCGCTCGCGCTGCTC
GTGGGCTTCGGCGTGTTCGCGGGGCTGCGCATCGCGCTCGCCGGCCATTCGGATCGGCTGTTCGCGTCGATCGACGCGCT
GCACGTGCCGAAGCTGCAGCCGGCGCCGCCCGCGCCGCATCCGGCGCCCGCGCCGCGCGTCGCGAAGTTCCTCGAGCCGG
AGATCGCCGCGGGGCTCGTGAGCGTGCGCGACGAGGCCGACCGCAGCGTGATCGTGCTGCGCGGCGACGGCCTGTTCGGC
TCCGGCTCGACGTCGGTGATCGATCGCTACATGCCGGTGCTCACGCGCGTGGCCGACGCGCTGAACCAGGTGCAGGGCAA
CGTGCGCGTGAGCGGCTACACCGACGACACGCCGGTGCACACCGCGCGCTTCGCGTCGAACTGGGATTTGTCGCGCGAGC
GCGCGCAGGCGGTCCGCAGCCTGATCGCCGCGCGGCTCGACCGCCCCGAGCGGATCACCGCCGAAGGGCGCGGCACGCTC
GATCCCGTCGCGCCGAACGATTCGCCCGCGAACCGCGCGCGCAACCGGCGCGTCGAGATCACGCTGATGCTCGCGCCCGG
CAGCGACGCCGCGCGCGCGACGAAGGAGGCGCCCTGA