Detailed information of component protein


Summary

Component ID T6CP000663
Component protein type TssM
T6SS ID (Type) T6SS00074 insolico (Type i3)
Strain Burkholderia pseudomallei K96243
Replicon chromosome II
Sequence Protein sequence (1168 a.a.); Nucleotide sequence (3504 bp)
Reference
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) BPS_RS21575
Coordinate (Strand) 724933..728436 (+)
NCBI ID WP_004190265.1
RefSeq NC_006351
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 2.1e-63 496..798
IcmF-related_N PF14331.8 1.7e-28 192..384
IcmF_C PF06744.14 7e-17 1046..1146
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-8 MIRTSLRV
Transmembrane helix 9-31 FAAILIAILIWWVGPLFAFGIYH
Outside 32-40 PLGPVWVRE
Transmembrane helix 41-60 ILVALVLIWGFWPTLARLWA
Inside 61-434 RLAMSPRQVKVAPKAKQLDFVDKHLRTLDQQLKERWRKEPRGRWKRWVGALTREHRTMLPWYLVLGSEGSGKSSLVAKAVSVSGSLQDRVLGSDATYGRGDDLNFRITREAVWFDVGGRWSLRAGADEAEFDAWRKLLRGMRRLRRGAPISGVVLCVDGLEMIDAPLDARKRLADSVRARLEEMREAFGQQVQVYVALNGLDRLDGAVSTLSLLEASKWVKGVGFSLPDDGAHADAARADASWQHALQGLQQRVQQQVLYSAPAATEVSMNHAQLRFVETLSRLQKGLVAWLHVALAPGEPHTAARLRGVWLGSMAELAEPHPAGAGGVGSAELPVPSRPLSELWTPLIRQVALERDAVRPSGPKSWRGRLGEA
Transmembrane helix 435-457 LRWGAVPLVALSLLLWFGWGYVT
Outside 458-1167 ERDYLDGVWAQFTEAKRLAQAEASYGNDGGSTLIEIANQMRYAQLQAEDAAQGMATPYFEHGLVAETARETYYRHLQKMLMPELYNEVRRTLVSQVDGSPGDIYQTLKVYLMLCRPARRSADDVVRWLDGRWDALSGGQYSDDDRRSLLAHVRTLMSLKEVPATPEDANLVRSARAKAAQIPLVTRVLQHIHAQGLPQQVNDISLSRAAGFEASMSLRMRSNVPSTDTAVSGWFTRAGYTDVFLPRLQKSARAMLEEESWVLRDETLSGNSFQIDGLVQKLADSARNQFLQDYISAWQNFLNDVTVRGVTGLDDASQLAAAMMEAQSPLANLLRFAARETTLTGASDEGNIDSWIDRQKYRFEKGRRQVVGELSGQHYRTVLLPEHVVEEHFQAIRQLAAQLNRNNTIANNPLSRLFEPLYRQLGLVNGALQAGQVLPAQYDAFSRLKETAARQPEPVRGIMLDLVSSGSTMTTRESGALLNRGAAGATKMVCDQGFTGRYPMRRGAQADAGVEDFERLFSAQGLMATYFRDHLAAYVDTSAKPWQALRSNGGPNGMVSQSVLNSYETAERIRGAMLDDSGHLRVSTVLRFIDMDSQLSEAQLSVAGQTVRFAHGVTSPHRVDWTNQNTQLAIKLQLKSVDGRMTTLQFDGPWALFRFFDAGQAVGGTGTADRRERLYQTSLGTVRIEWQALTLPSPIWSGILQSFRCPS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9979 - -


  Protein sequence: 1168 a.a.    

>T6CP000663 NC_006351:724933-728436 [Burkholderia pseudomallei K96243] [TssM]
MIRTSLRVFAAILIAILIWWVGPLFAFGIYHPLGPVWVREILVALVLIWGFWPTLARLWARLAMSPRQVKVAPKAKQLDF
VDKHLRTLDQQLKERWRKEPRGRWKRWVGALTREHRTMLPWYLVLGSEGSGKSSLVAKAVSVSGSLQDRVLGSDATYGRG
DDLNFRITREAVWFDVGGRWSLRAGADEAEFDAWRKLLRGMRRLRRGAPISGVVLCVDGLEMIDAPLDARKRLADSVRAR
LEEMREAFGQQVQVYVALNGLDRLDGAVSTLSLLEASKWVKGVGFSLPDDGAHADAARADASWQHALQGLQQRVQQQVLY
SAPAATEVSMNHAQLRFVETLSRLQKGLVAWLHVALAPGEPHTAARLRGVWLGSMAELAEPHPAGAGGVGSAELPVPSRP
LSELWTPLIRQVALERDAVRPSGPKSWRGRLGEALRWGAVPLVALSLLLWFGWGYVTERDYLDGVWAQFTEAKRLAQAEA
SYGNDGGSTLIEIANQMRYAQLQAEDAAQGMATPYFEHGLVAETARETYYRHLQKMLMPELYNEVRRTLVSQVDGSPGDI
YQTLKVYLMLCRPARRSADDVVRWLDGRWDALSGGQYSDDDRRSLLAHVRTLMSLKEVPATPEDANLVRSARAKAAQIPL
VTRVLQHIHAQGLPQQVNDISLSRAAGFEASMSLRMRSNVPSTDTAVSGWFTRAGYTDVFLPRLQKSARAMLEEESWVLR
DETLSGNSFQIDGLVQKLADSARNQFLQDYISAWQNFLNDVTVRGVTGLDDASQLAAAMMEAQSPLANLLRFAARETTLT
GASDEGNIDSWIDRQKYRFEKGRRQVVGELSGQHYRTVLLPEHVVEEHFQAIRQLAAQLNRNNTIANNPLSRLFEPLYRQ
LGLVNGALQAGQVLPAQYDAFSRLKETAARQPEPVRGIMLDLVSSGSTMTTRESGALLNRGAAGATKMVCDQGFTGRYPM
RRGAQADAGVEDFERLFSAQGLMATYFRDHLAAYVDTSAKPWQALRSNGGPNGMVSQSVLNSYETAERIRGAMLDDSGHL
RVSTVLRFIDMDSQLSEAQLSVAGQTVRFAHGVTSPHRVDWTNQNTQLAIKLQLKSVDGRMTTLQFDGPWALFRFFDAGQ
AVGGTGTADRRERLYQTSLGTVRIEWQALTLPSPIWSGILQSFRCPS*

  Nucleotide sequence: 3504 bp    

>T6CP000663 NC_006351:724933-728436 [Burkholderia pseudomallei K96243] [TssM]
ATGATCCGCACGAGCTTGAGAGTATTCGCGGCGATTCTGATCGCGATCCTGATCTGGTGGGTGGGACCGCTGTTCGCGTT
CGGCATCTATCACCCGCTCGGCCCGGTGTGGGTGCGCGAGATCCTCGTCGCACTGGTGCTGATCTGGGGCTTCTGGCCGA
CGCTCGCGCGGCTCTGGGCGCGGCTCGCGATGAGCCCGCGGCAGGTGAAGGTCGCGCCGAAGGCCAAGCAGCTCGATTTC
GTCGACAAGCATCTGCGCACCCTCGACCAGCAGTTGAAGGAGCGCTGGCGCAAGGAGCCGCGCGGCCGCTGGAAGCGCTG
GGTCGGCGCGCTCACGCGCGAGCATCGCACGATGCTGCCGTGGTATCTGGTGCTCGGCTCCGAGGGTAGCGGCAAGTCGA
GCCTCGTCGCGAAGGCGGTGAGCGTGTCCGGCTCGCTGCAGGACCGGGTGCTCGGCTCGGACGCGACCTACGGGCGCGGC
GACGACTTGAACTTCCGCATCACGCGCGAGGCGGTGTGGTTCGACGTCGGCGGCCGCTGGAGCCTGCGCGCGGGCGCCGA
CGAGGCCGAGTTCGACGCGTGGCGCAAGCTGCTGCGCGGGATGAGGCGGCTGCGCAGGGGCGCGCCGATCAGCGGCGTCG
TGCTGTGCGTCGACGGCCTCGAGATGATCGATGCGCCGCTCGACGCGCGCAAGCGGCTGGCCGATTCGGTGCGCGCGCGG
CTCGAGGAGATGCGCGAGGCGTTCGGCCAGCAGGTGCAGGTGTACGTTGCGCTGAACGGGCTCGACCGGCTCGACGGCGC
GGTCTCGACGCTGTCGCTGCTCGAGGCGTCGAAGTGGGTCAAGGGCGTGGGCTTCAGCCTGCCCGACGACGGCGCGCACG
CCGACGCCGCGCGCGCGGACGCGAGCTGGCAGCACGCGCTGCAAGGGCTGCAGCAGCGGGTCCAGCAGCAGGTGCTGTAT
TCGGCGCCGGCGGCGACCGAGGTGTCGATGAACCACGCGCAGTTGCGCTTCGTCGAGACGCTCAGCCGCCTGCAGAAGGG
GCTCGTCGCGTGGCTGCACGTCGCGCTCGCGCCGGGCGAGCCGCACACGGCCGCGCGGCTGCGCGGGGTGTGGCTGGGCT
CGATGGCCGAGCTCGCGGAGCCGCATCCGGCGGGCGCGGGCGGCGTCGGCTCGGCGGAGCTGCCCGTGCCGTCGCGCCCG
CTCAGCGAGCTGTGGACGCCGCTCATCCGGCAGGTGGCGCTCGAGCGCGACGCGGTGCGCCCGAGCGGGCCGAAGTCGTG
GCGCGGGCGCCTGGGCGAGGCGCTGCGCTGGGGCGCGGTGCCCCTCGTCGCGCTGAGCCTGCTGCTGTGGTTCGGCTGGG
GCTACGTCACCGAGCGCGACTATCTGGACGGCGTCTGGGCGCAGTTCACCGAGGCCAAGCGGCTCGCGCAGGCCGAGGCG
TCCTACGGCAACGACGGCGGCTCGACGCTCATCGAGATCGCGAACCAGATGCGCTATGCGCAACTGCAGGCCGAGGACGC
CGCGCAGGGCATGGCGACGCCGTACTTCGAGCACGGGCTCGTCGCGGAAACCGCGCGCGAGACCTACTACCGGCACTTGC
AGAAGATGCTGATGCCGGAGCTGTACAACGAGGTGCGGCGCACGCTCGTGTCGCAGGTCGACGGCAGCCCGGGCGACATC
TACCAGACGCTGAAGGTCTACCTGATGCTCTGCCGCCCCGCGCGGCGCTCGGCGGACGACGTCGTGCGCTGGCTCGACGG
CCGCTGGGACGCGCTCTCGGGCGGGCAGTACTCGGACGACGACCGCCGCTCGCTGCTCGCGCACGTGCGCACGCTGATGT
CGCTGAAGGAGGTGCCGGCCACGCCGGAGGACGCGAACCTCGTGCGCTCGGCGCGCGCGAAGGCCGCGCAGATCCCGCTC
GTCACGCGCGTGCTGCAGCACATCCACGCGCAGGGGCTGCCGCAGCAGGTCAACGACATTTCGCTGTCGCGCGCGGCGGG
CTTCGAGGCGTCGATGAGCCTGCGCATGCGCAGCAACGTGCCGTCGACCGACACCGCGGTGTCGGGCTGGTTCACGCGCG
CGGGCTACACGGACGTGTTCCTGCCGCGCCTGCAGAAGAGCGCGCGCGCGATGCTCGAGGAGGAGAGCTGGGTGCTGCGC
GACGAGACGCTGTCGGGCAACAGCTTCCAGATCGACGGACTCGTGCAGAAGCTCGCCGATTCGGCGCGCAACCAGTTCCT
GCAGGACTACATCTCCGCGTGGCAGAACTTCCTGAACGACGTGACGGTGCGCGGCGTGACGGGCCTGGACGATGCGTCGC
AGCTCGCCGCGGCGATGATGGAGGCGCAATCGCCGCTCGCGAACCTGCTGCGCTTCGCCGCGCGCGAGACGACGCTCACG
GGCGCGAGCGACGAAGGCAACATCGACAGCTGGATCGATCGCCAGAAGTACCGCTTCGAGAAGGGGCGGCGGCAGGTCGT
CGGCGAGCTGAGCGGGCAGCATTACCGCACGGTGCTGCTGCCGGAGCACGTGGTCGAGGAGCACTTCCAGGCGATCCGCC
AGCTCGCCGCGCAACTGAACCGCAACAACACGATCGCGAACAACCCGCTGTCGCGGCTGTTCGAGCCGCTGTACCGGCAG
CTCGGGCTCGTCAACGGCGCGCTGCAGGCGGGCCAGGTGCTGCCCGCGCAGTACGACGCGTTCTCACGGCTGAAGGAGAC
GGCCGCGCGCCAGCCGGAGCCGGTGCGCGGGATCATGCTCGACCTCGTGAGCAGCGGCAGCACGATGACGACGCGCGAAT
CGGGCGCGCTGCTCAACCGGGGCGCGGCGGGCGCGACGAAGATGGTCTGCGATCAGGGCTTCACGGGCCGCTATCCGATG
CGCCGCGGCGCGCAGGCGGATGCGGGCGTGGAGGACTTCGAGCGGCTGTTCAGCGCGCAGGGGCTGATGGCGACGTACTT
CCGCGATCACCTCGCCGCGTATGTGGACACATCGGCCAAGCCGTGGCAGGCGCTGCGCTCGAACGGCGGGCCGAACGGGA
TGGTGAGCCAGTCGGTGCTGAACTCGTACGAGACGGCCGAGCGTATCCGCGGCGCGATGCTCGACGATTCGGGGCACCTG
CGCGTGTCGACCGTGCTGCGTTTCATCGACATGGATTCGCAGTTGTCCGAAGCGCAGCTGAGCGTGGCTGGGCAGACGGT
GCGCTTCGCGCACGGCGTGACCTCGCCGCACCGGGTGGACTGGACCAACCAGAACACGCAGCTCGCGATCAAGCTGCAGC
TCAAATCGGTGGACGGCCGGATGACGACGCTGCAGTTCGACGGGCCGTGGGCGCTGTTCCGCTTCTTCGACGCGGGGCAG
GCGGTGGGCGGCACGGGCACCGCGGACCGGCGCGAGCGGCTGTATCAGACGAGCCTCGGCACGGTGCGCATCGAATGGCA
GGCGCTCACGCTGCCGTCGCCGATATGGTCGGGCATCCTGCAGTCGTTCAGGTGTCCGTCGTGA