SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000657
Component protein type	TssG
T6SS ID (Type)	T6SS00074 (Type i3)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome II
Sequence	Protein sequence (330 a.a.); Nucleotide sequence (990 bp)
Reference	[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPS_RS21520
Coordinate (Strand)	708199..709188 (+)
NCBI ID	WP_004190851.1
RefSeq	NC_006351
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	7.5e-56	14..311

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-329	MTPLERVSRALTPRRTFFELMRRVEALQRRHDGRSARKRRMPKWLRIEQPAQMHFASTEVERVQVTLARFVEDDDHPQVTVAQRHFGLFAPYGPLPLHVTEHAMQEKRFERNAAFERFVNVACGDLAWLHYSAWSSMHPVLGYERARNPFVERVTALADACRAQQDDGEPYGRHALACRRAFPGIYCAPRRSLADLQRLLRAYFGVALQVVPRHGRWVPVPAAASHARRLGGWRLGARIWDVQHSIEIVVGPIEADEFYRWQRRAAAVMALSAVVTDFVDGRIYPVIKVQVWTRPELAGRVGCMRVGVDAWSRPNRALRTLTVFESFRD

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9584	-	-

Protein sequence: 330 a.a.

						>T6CP000657 NC_006351:708199-709188 [Burkholderia pseudomallei K96243] [TssG]
MTPLERVSRALTPRRTFFELMRRVEALQRRHDGRSARKRRMPKWLRIEQPAQMHFASTEVERVQVTLARFVEDDDHPQVT
VAQRHFGLFAPYGPLPLHVTEHAMQEKRFERNAAFERFVNVACGDLAWLHYSAWSSMHPVLGYERARNPFVERVTALADA
CRAQQDDGEPYGRHALACRRAFPGIYCAPRRSLADLQRLLRAYFGVALQVVPRHGRWVPVPAAASHARRLGGWRLGARIW
DVQHSIEIVVGPIEADEFYRWQRRAAAVMALSAVVTDFVDGRIYPVIKVQVWTRPELAGRVGCMRVGVDAWSRPNRALRT
LTVFESFRD*					

Nucleotide sequence: 990 bp

						>T6CP000657 NC_006351:708199-709188 [Burkholderia pseudomallei K96243] [TssG]
ATGACGCCGCTCGAGCGCGTGAGCCGGGCGCTGACGCCGCGCCGCACGTTCTTCGAGCTGATGCGGCGCGTCGAGGCGCT
GCAGCGCAGGCACGACGGGCGTTCGGCGCGCAAGCGCCGGATGCCGAAGTGGCTGCGCATCGAGCAGCCGGCGCAGATGC
ACTTCGCGAGCACGGAAGTCGAGCGCGTGCAGGTGACGCTCGCGCGCTTCGTCGAGGACGACGATCACCCGCAAGTCACG
GTGGCGCAGCGCCATTTCGGGCTGTTCGCGCCGTACGGGCCGCTGCCGCTGCACGTGACCGAGCACGCGATGCAGGAGAA
GCGCTTCGAGCGCAACGCCGCGTTCGAGCGCTTCGTAAACGTCGCGTGCGGCGATCTCGCGTGGCTGCATTACTCGGCGT
GGTCGTCGATGCATCCGGTGCTCGGCTACGAGCGCGCGCGCAATCCGTTCGTCGAGCGCGTGACGGCGCTCGCCGATGCG
TGCCGCGCGCAGCAGGACGACGGCGAGCCGTACGGGCGGCACGCGCTCGCATGCCGGCGCGCGTTTCCGGGCATCTACTG
CGCGCCGCGTCGCTCGCTGGCCGATCTGCAGCGGCTGCTGCGCGCGTACTTCGGCGTGGCGCTGCAGGTCGTGCCGCGCC
ACGGGCGCTGGGTGCCGGTGCCGGCCGCGGCGAGCCATGCGCGGCGGCTCGGCGGCTGGCGGCTGGGTGCGCGGATCTGG
GACGTGCAGCACTCGATCGAGATCGTGGTGGGGCCGATCGAGGCGGACGAGTTCTACCGGTGGCAACGCCGCGCGGCCGC
CGTGATGGCGCTGTCGGCGGTCGTGACGGATTTCGTCGACGGACGGATTTACCCCGTCATCAAGGTTCAGGTGTGGACGC
GCCCTGAACTCGCAGGAAGGGTTGGGTGCATGCGTGTCGGGGTCGACGCGTGGTCACGGCCGAATCGGGCGCTGCGCACC
TTGACTGTCTTTGAATCTTTTCGGGACTAG					