SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000649
Component protein type	TssA
T6SS ID (Type)	T6SS00073 (Type i3)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome II
Sequence	Protein sequence (360 a.a.); Nucleotide sequence (1080 bp)
Reference	[1] Burtnick MN, Brett PJ, Harding SV, et al. The cluster 1 type VI secretion system is a major virulence determinant in Burkholderia pseudomallei. Infect Immun. 2011 Apr;79(4):1512-25. doi: 10.1128/IAI.01218-10. Epub 2011 Feb 7. PMID: 21300775

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPS_RS19560
Coordinate (Strand)	240376..241455 (+)
NCBI ID	WP_172966718.1
RefSeq	NC_006351
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	7.1e-32	1..126

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-359	MAPVDAAAPCGADLEYDPEFVVLAAKVAPRAEAQYGDFVGSPEPVNWSDVERDCRRLMMRSKDMRLAVLFARSRTRLAGATGLAEGIGLLAAWLAAFPDAIHPQADVDADRDAALEIRRNALQALTDADGLLADVREIALTRSSATRLQVRDVERAFAQPRPGDALAPESVVRQLDDLHAQQPETLAGFGDALAGLAAIDAWSGEHLGDYAPDLSALDALLRRIAGANARGDRAEAEPIAPAEADAPPASEAAAAHAHAHAHAHAHAHAHAHASPPRRREPAAQALAAAIAGEPAAQPVDRYAARELIRQARQWFEQHEPSSPIPILLRRAEHFVGKRYADVVQAIPAELLALWSADET

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9622	-	-

Protein sequence: 360 a.a.

						>T6CP000649 NC_006351:240376-241455 [Burkholderia pseudomallei K96243] [TssA]
MAPVDAAAPCGADLEYDPEFVVLAAKVAPRAEAQYGDFVGSPEPVNWSDVERDCRRLMMRSKDMRLAVLFARSRTRLAGA
TGLAEGIGLLAAWLAAFPDAIHPQADVDADRDAALEIRRNALQALTDADGLLADVREIALTRSSATRLQVRDVERAFAQP
RPGDALAPESVVRQLDDLHAQQPETLAGFGDALAGLAAIDAWSGEHLGDYAPDLSALDALLRRIAGANARGDRAEAEPIA
PAEADAPPASEAAAAHAHAHAHAHAHAHAHAHASPPRRREPAAQALAAAIAGEPAAQPVDRYAARELIRQARQWFEQHEP
SSPIPILLRRAEHFVGKRYADVVQAIPAELLALWSADET*					

Nucleotide sequence: 1080 bp

						>T6CP000649 NC_006351:240376-241455 [Burkholderia pseudomallei K96243] [TssA]
ATGGCGCCCGTCGATGCGGCCGCGCCGTGCGGAGCGGACCTTGAATACGATCCCGAGTTCGTCGTGCTCGCGGCGAAGGT
CGCGCCGCGCGCCGAAGCGCAGTACGGGGACTTCGTCGGCTCGCCGGAGCCGGTGAACTGGAGCGACGTCGAGCGCGACT
GCCGACGGCTGATGATGCGCAGCAAGGACATGCGCCTCGCGGTGCTGTTCGCGCGCAGCCGCACGCGGCTTGCCGGCGCG
ACCGGGCTCGCGGAAGGCATTGGCTTGCTGGCCGCGTGGCTCGCCGCGTTTCCCGATGCGATTCATCCGCAAGCCGACGT
CGACGCGGACCGCGACGCGGCGCTCGAGATCCGGCGCAACGCGCTGCAGGCGCTGACCGATGCCGACGGGCTGCTGGCGG
ACGTGCGCGAGATCGCGCTGACCCGCTCGAGCGCGACGCGCCTGCAGGTGCGCGACGTGGAACGCGCGTTCGCGCAACCG
CGGCCCGGCGATGCGCTCGCGCCGGAATCGGTCGTGCGCCAGCTCGACGATTTGCACGCGCAGCAGCCGGAGACGCTCGC
GGGCTTCGGCGATGCGCTCGCGGGGCTGGCCGCGATCGACGCCTGGAGCGGCGAGCATCTCGGCGATTATGCGCCGGACC
TGTCGGCGCTCGATGCGCTGCTGCGCCGGATCGCGGGCGCGAACGCGCGCGGCGATCGCGCGGAGGCCGAGCCGATCGCG
CCGGCCGAAGCCGATGCGCCGCCAGCGAGCGAGGCGGCGGCCGCGCACGCGCACGCGCACGCGCACGCACACGCACACGC
ACACGCGCACGCACACGCATCGCCGCCGCGGCGGCGCGAGCCCGCGGCGCAGGCACTCGCGGCTGCCATTGCGGGCGAAC
CGGCCGCGCAGCCGGTGGATCGTTACGCGGCGCGCGAGCTGATCCGGCAGGCGCGCCAGTGGTTCGAGCAGCACGAGCCG
AGCAGCCCGATTCCGATTCTGCTCAGGCGCGCGGAGCATTTCGTCGGCAAGCGCTATGCGGATGTGGTGCAGGCGATTCC
CGCCGAGCTGCTCGCGCTGTGGAGCGCGGACGAAACCTGA					