SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000628
Component protein type	TssJ
T6SS ID (Type)	T6SS00072 (Type i3)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome II
Sequence	Protein sequence (181 a.a.); Nucleotide sequence (543 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPS_RS19115
Coordinate (Strand)	120611..121153 (+)
NCBI ID	WP_004551171.1
RefSeq	NC_006351
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.3e-34	44..169

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-180	MAFPFFFRREAARRTAVKRVVAAMVCTSLAACASLPARKETADLIVKIKVSEGANPDEHQRPAPVMVRLYELKSAGAFENADFFTLQSDSRKVLGDDAIATDEFVMRPGDTRDIHRRADSAGTSVGVLVGYRELGKSVWRAVHKLRPAPEDAWYRAFTPRTKIKLNVDVGQQTVTITELE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.8309	pos: 31-32. LAA-CA.	MAFPFFFRREAARRTAVKRVVAAMVCTSLAA

Protein sequence: 181 a.a.

						>T6CP000628 NC_006351:120611-121153 [Burkholderia pseudomallei K96243] [TssJ]
MAFPFFFRREAARRTAVKRVVAAMVCTSLAACASLPARKETADLIVKIKVSEGANPDEHQRPAPVMVRLYELKSAGAFEN
ADFFTLQSDSRKVLGDDAIATDEFVMRPGDTRDIHRRADSAGTSVGVLVGYRELGKSVWRAVHKLRPAPEDAWYRAFTPR
TKIKLNVDVGQQTVTITELE*					

Nucleotide sequence: 543 bp

						>T6CP000628 NC_006351:120611-121153 [Burkholderia pseudomallei K96243] [TssJ]
ATGGCTTTCCCATTTTTCTTTCGCCGCGAAGCGGCCAGGCGAACCGCCGTCAAGCGCGTTGTGGCGGCAATGGTATGCAC
ATCGCTCGCGGCTTGCGCAAGCTTGCCGGCACGCAAGGAAACAGCCGATCTGATCGTGAAGATCAAGGTGAGCGAAGGCG
CCAATCCGGATGAGCATCAGCGCCCGGCGCCCGTGATGGTGCGGCTGTACGAGCTGAAATCGGCCGGCGCGTTCGAGAAC
GCCGATTTTTTCACGCTACAGAGCGACAGCCGCAAGGTGTTGGGTGACGACGCGATCGCGACGGATGAATTCGTGATGCG
CCCGGGCGACACCCGGGATATTCATCGGCGTGCCGATTCCGCGGGGACGTCGGTCGGCGTGCTGGTCGGTTATCGAGAGC
TCGGCAAATCGGTGTGGCGGGCGGTCCATAAGCTGCGGCCGGCTCCGGAGGATGCATGGTACCGGGCATTCACGCCAAGG
ACGAAAATCAAGTTGAACGTCGATGTCGGGCAGCAGACGGTGACGATTACAGAATTGGAATGA					