SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000625
Component protein type	TssB
T6SS ID (Type)	T6SS00072 (Type i3)
Strain	Burkholderia pseudomallei K96243
Replicon	chromosome II
Sequence	Protein sequence (180 a.a.); Nucleotide sequence (540 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BPS_RS19100
Coordinate (Strand)	117729..118268 (+)
NCBI ID	WP_004523700.1
RefSeq	NC_006351
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	2.1e-58	12..162

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-179	MAISNSSQKFIARNRAPRVQIEYDVEIYGSEKKVELPFVMGVLADLSGKPLEPLPAVGDRKFFSIDIDNFDERMKAMKPRVAFSVPNTLSGDGQLMVDITFESMDDFSPAAIAKKVDALSQLLDARTQLANLQTYMDGKSGAENLVSKVLKDPALLSALAKAPKPVAEQAENRESKEKH

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9956	-	-

Protein sequence: 180 a.a.

						>T6CP000625 NC_006351:117729-118268 [Burkholderia pseudomallei K96243] [TssB]
MAISNSSQKFIARNRAPRVQIEYDVEIYGSEKKVELPFVMGVLADLSGKPLEPLPAVGDRKFFSIDIDNFDERMKAMKPR
VAFSVPNTLSGDGQLMVDITFESMDDFSPAAIAKKVDALSQLLDARTQLANLQTYMDGKSGAENLVSKVLKDPALLSALA
KAPKPVAEQAENRESKEKH*					

Nucleotide sequence: 540 bp

						>T6CP000625 NC_006351:117729-118268 [Burkholderia pseudomallei K96243] [TssB]
ATGGCTATATCCAATAGCTCACAGAAATTTATCGCGCGCAATCGTGCGCCTCGCGTTCAGATCGAATATGACGTCGAGAT
TTACGGCTCCGAAAAGAAGGTCGAGCTTCCGTTCGTGATGGGCGTGCTCGCGGACCTGTCGGGCAAGCCGCTCGAGCCTC
TGCCGGCGGTCGGCGACCGCAAGTTCTTCAGCATCGATATCGACAATTTCGACGAGCGCATGAAGGCGATGAAGCCGCGT
GTCGCGTTCTCGGTGCCGAATACTCTTTCCGGCGACGGTCAATTGATGGTCGACATCACGTTCGAAAGCATGGACGACTT
TTCTCCCGCGGCGATCGCGAAGAAGGTGGATGCGTTGTCGCAATTGCTGGACGCGCGCACGCAGCTTGCCAATCTGCAGA
CCTACATGGACGGAAAGTCGGGCGCGGAGAACCTCGTGAGCAAGGTGCTCAAGGACCCCGCGCTGCTGAGCGCGCTTGCG
AAGGCGCCGAAGCCCGTGGCGGAACAGGCGGAAAACAGGGAATCGAAGGAAAAGCACTGA					