SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000610
Component protein type	TssJ
T6SS ID (Type)	T6SS00071 (Type i3)
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II
Sequence	Protein sequence (168 a.a.); Nucleotide sequence (504 bp)
Reference	[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BMA_RS25085
Coordinate (Strand)	2096218..2096721 (+)
NCBI ID	WP_004187276.1
RefSeq	NC_006349
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	1.7e-38	31..156

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-167	MHPTTPVAAVALALACAACASGAAKQKEPLRLELAVRAAPTVNPDDRSRPAPIVVSVYELKTDGAFNAADFFTLQGPDKTVLADDLLARERFQLRPGERRVIRRDADPAAGTLGVVAAYRDLPNSVWRAAYPLPPARDAAWYRFSSPKLKLTIELDTHAIRITEPGK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9386	pos: 18-19. CAA-CA.	MHPTTPVAAVALALACAA

Protein sequence: 168 a.a.

						>T6CP000610 NC_006349:2096218-2096721 [Burkholderia mallei ATCC 23344] [TssJ]
MHPTTPVAAVALALACAACASGAAKQKEPLRLELAVRAAPTVNPDDRSRPAPIVVSVYELKTDGAFNAADFFTLQGPDKT
VLADDLLARERFQLRPGERRVIRRDADPAAGTLGVVAAYRDLPNSVWRAAYPLPPARDAAWYRFSSPKLKLTIELDTHAI
RITEPGK*					

Nucleotide sequence: 504 bp

						>T6CP000610 NC_006349:2096218-2096721 [Burkholderia mallei ATCC 23344] [TssJ]
ATGCATCCGACCACCCCCGTTGCCGCCGTCGCGCTCGCGCTCGCGTGCGCCGCGTGCGCGAGCGGCGCGGCCAAGCAGAA
GGAGCCGCTGCGGCTCGAGCTCGCCGTGCGCGCCGCGCCCACGGTCAATCCGGACGATCGGAGCCGGCCGGCTCCGATCG
TCGTGAGCGTCTACGAGCTGAAGACCGACGGCGCATTCAACGCCGCCGATTTCTTCACGCTGCAGGGCCCGGACAAGACC
GTGCTCGCCGACGATCTGCTCGCGCGCGAGCGTTTTCAACTGCGCCCCGGCGAGCGCCGCGTGATCCGGCGCGACGCCGA
TCCGGCGGCGGGCACGCTCGGCGTGGTCGCCGCGTACCGCGATCTGCCGAACTCGGTGTGGCGCGCGGCATATCCGCTGC
CGCCCGCGCGGGACGCCGCATGGTATCGCTTCTCGTCGCCGAAGCTGAAGCTGACCATCGAGCTCGACACGCACGCGATC
AGGATCACCGAACCCGGAAAATGA					