SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000604
Component protein type	TssA
T6SS ID (Type)	T6SS00071 (Type i3)
Strain	Burkholderia mallei ATCC 23344
Replicon	chromosome II
Sequence	Protein sequence (368 a.a.); Nucleotide sequence (1104 bp)
Reference	[1] Schell MA, Ulrich RL, Ribot WJ, et al. Type VI secretion is a major virulence determinant in Burkholderia mallei. Mol Microbiol. 2007 Jun;64(6):1466-85. PMID: 17555434

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	BMA_RS25035
Coordinate (Strand)	2083549..2084652 (-)
NCBI ID	WP_004196241.1
RefSeq	NC_006349
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	7.5e-32	23..148

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-367	MTPSRKPAGRAAAARTPKPDGWMAPVDAAAPCGADLEYDPEFVVLAAKVAPRAEAQYGDFVGSPEPVNWSDVERDCRRLMMRSKDMRLAVLFARSRTRLAGATGLAEGIGLLAAWLAAFPDAIHPQADVDADRDAALEIRRNALQALTDADGLLADVREIALTRSSATRLQVRDVERAFAQPRPGDALAPESVVRQLDDLHAQQPETLAGFGDALAGLAAIDAWSGEHLGDYAPDLSALDALLRRIAGANARGDRAEAEPIAPAEADAPPASEAAAAHAHASPPRRREPAAQALAAAIAGEPAAQPVDRYAARELIRQARQWFEQHEPSSPIPILLRRAEHFVGKRYADVVQAIPAELLALWSADET

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.8443	-	-

Protein sequence: 368 a.a.

						>T6CP000604 NC_006349:c2084652-2083549 [Burkholderia mallei ATCC 23344] [TssA]
MTPSRKPAGRAAAARTPKPDGWMAPVDAAAPCGADLEYDPEFVVLAAKVAPRAEAQYGDFVGSPEPVNWSDVERDCRRLM
MRSKDMRLAVLFARSRTRLAGATGLAEGIGLLAAWLAAFPDAIHPQADVDADRDAALEIRRNALQALTDADGLLADVREI
ALTRSSATRLQVRDVERAFAQPRPGDALAPESVVRQLDDLHAQQPETLAGFGDALAGLAAIDAWSGEHLGDYAPDLSALD
ALLRRIAGANARGDRAEAEPIAPAEADAPPASEAAAAHAHASPPRRREPAAQALAAAIAGEPAAQPVDRYAARELIRQAR
QWFEQHEPSSPIPILLRRAEHFVGKRYADVVQAIPAELLALWSADET*					

Nucleotide sequence: 1104 bp

						>T6CP000604 NC_006349:c2084652-2083549 [Burkholderia mallei ATCC 23344] [TssA]
ATGACCCCCAGCAGAAAACCGGCCGGCAGGGCGGCTGCCGCGCGTACGCCGAAGCCCGACGGCTGGATGGCGCCCGTCGA
TGCGGCCGCGCCGTGCGGAGCGGACCTTGAATACGATCCCGAGTTCGTCGTGCTCGCGGCGAAGGTCGCGCCGCGCGCCG
AAGCGCAGTACGGGGACTTCGTCGGCTCGCCGGAGCCGGTGAACTGGAGCGACGTCGAGCGCGACTGCCGACGGCTGATG
ATGCGCAGCAAGGACATGCGCCTCGCGGTGCTGTTCGCGCGCAGCCGCACGCGGCTTGCCGGCGCGACCGGGCTCGCGGA
AGGCATTGGCTTGCTGGCCGCGTGGCTCGCCGCGTTTCCCGATGCGATTCATCCGCAAGCCGACGTCGACGCGGACCGCG
ACGCGGCGCTCGAGATCCGGCGCAACGCGCTGCAGGCGCTGACCGATGCCGACGGGCTGCTGGCGGACGTGCGCGAGATC
GCGCTGACCCGCTCGAGCGCGACGCGCCTGCAGGTGCGCGACGTGGAACGCGCGTTCGCGCAACCGCGGCCCGGCGATGC
GCTCGCGCCGGAATCGGTCGTGCGCCAGCTCGACGATTTGCACGCGCAGCAGCCGGAGACGCTCGCGGGCTTCGGCGATG
CGCTCGCGGGGCTGGCCGCGATCGACGCCTGGAGCGGCGAGCATCTCGGCGATTATGCGCCGGACCTGTCGGCGCTCGAT
GCGCTGCTTCGCCGGATCGCGGGCGCGAACGCGCGCGGCGATCGCGCGGAGGCCGAGCCGATCGCGCCGGCCGAAGCCGA
TGCGCCGCCGGCGAGCGAGGCGGCGGCCGCGCACGCGCACGCATCGCCGCCGCGGCGGCGCGAGCCCGCGGCGCAGGCAC
TCGCGGCTGCCATTGCGGGCGAACCGGCCGCGCAGCCGGTGGATCGTTACGCGGCGCGCGAGCTGATCCGGCAGGCGCGC
CAGTGGTTCGAGCAGCACGAGCCGAGCAGCCCGATTCCGATTCTGCTCAGGCGCGCGGAGCATTTCGTCGGCAAGCGCTA
TGCGGATGTGGTGCAGGCGATTCCCGCCGAGCTGCTCGCGCTGTGGAGCGCGGACGAAACCTGA					