Detailed information of component protein


Summary

Component ID T6CP000529
Component protein type TssK
T6SS ID (Type) T6SS00061 experimental (Type i4b)
Strain Acinetobacter sp. ADP1
Replicon chromosome
Sequence Protein sequence (456 a.a.); Nucleotide sequence (1368 bp)
Reference
[1] Basler M, Ho BT, Mekalanos JJ. Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions. Cell. 2013 Feb 14;152(4):884-94. doi: 10.1016/j.cell.2013.01.042. PMID: 23415234
[2] Weber BS, Miyata ST, Iwashkiw JA, et al. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One. 2013;8(1):e55142. doi: 10.1371/journal.pone.0055142. Epub 2013 Jan 24. PMID: 23365692
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Shneider MM, Buth SA, Ho BT, et al. PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 2013 Aug 15;500(7462):350-353. doi: 10.1038/nature12453. Epub 2013 Aug 7. PMID: 23925114
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACIAD_RS12260
Coordinate (Strand) 2653945..2655312 (+)
NCBI ID WP_004929024.1
RefSeq NC_005966
Uniprot ID Q6F916_ACIAD
KEGG ID aci:ACIAD2696
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 5.6e-129 19..452
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-455 MFKAEKILWGEGLFLRPQHFQIQDSYHEQRLNQTIRATIPFAYGINQLRFDELQLGNHVLALESIEMIWQDGEIYQAPSTDLLPEPLLLDDLNFKGEMLIYLTLPLLQPNKTNILHDGDDHRSGRYHSHLTETHDLYTNAQAADITLLRRRAMFRLLAPLSEPQQEFDGLLYLPLGRIKRQSSGSFELDQKFIPPILHIEASDTLTSNLKRTLNVIRAKIKTIQTNNRENEQKLIEFRSGDIVSFWLVNALNTAHATLNHLLQHPQIHPERLFFELLRLTGSLLTFSTAYEVDHLPQYRHHQLQDSFQQLDVILRDLLDTIISSRYISIALKEIRPSYWIGSLESDKITRDSRLYIAVSSSMMQTHELVQIVPLRFKVGSTVDVEQRVVAALPAIPLHHLMQVPTAIPVRSGVSYFEIEPHHELYQRMLESESICIYIPSGFQDISIELIAVMNA


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9962 - -


  Protein sequence: 456 a.a.    

>T6CP000529 NC_005966:2653945-2655312 [Acinetobacter baylyi ADP1] [TssK]
MFKAEKILWGEGLFLRPQHFQIQDSYHEQRLNQTIRATIPFAYGINQLRFDELQLGNHVLALESIEMIWQDGEIYQAPST
DLLPEPLLLDDLNFKGEMLIYLTLPLLQPNKTNILHDGDDHRSGRYHSHLTETHDLYTNAQAADITLLRRRAMFRLLAPL
SEPQQEFDGLLYLPLGRIKRQSSGSFELDQKFIPPILHIEASDTLTSNLKRTLNVIRAKIKTIQTNNRENEQKLIEFRSG
DIVSFWLVNALNTAHATLNHLLQHPQIHPERLFFELLRLTGSLLTFSTAYEVDHLPQYRHHQLQDSFQQLDVILRDLLDT
IISSRYISIALKEIRPSYWIGSLESDKITRDSRLYIAVSSSMMQTHELVQIVPLRFKVGSTVDVEQRVVAALPAIPLHHL
MQVPTAIPVRSGVSYFEIEPHHELYQRMLESESICIYIPSGFQDISIELIAVMNA*

  Nucleotide sequence: 1368 bp    

>T6CP000529 NC_005966:2653945-2655312 [Acinetobacter baylyi ADP1] [TssK]
ATGTTTAAAGCAGAAAAAATCCTATGGGGCGAAGGCTTATTTTTACGTCCGCAACATTTCCAGATTCAGGATAGTTACCA
TGAGCAACGTTTAAACCAGACCATTCGTGCGACCATTCCGTTCGCCTATGGGATTAATCAATTGCGTTTCGACGAGCTGC
AACTGGGCAATCATGTACTGGCATTAGAAAGCATCGAAATGATCTGGCAAGACGGCGAAATTTACCAAGCCCCAAGCACC
GACTTATTACCAGAACCTTTGCTACTCGATGACCTCAATTTTAAGGGAGAGATGTTGATCTATCTCACCCTGCCGTTATT
ACAACCCAATAAGACAAATATCTTGCATGATGGTGATGATCATCGATCTGGCCGTTATCACTCACATCTAACCGAAACAC
ACGATTTGTATACCAATGCTCAGGCTGCAGACATTACGCTGTTACGGCGTCGTGCAATGTTTAGATTGCTTGCACCTCTG
TCCGAGCCACAACAAGAATTTGATGGTTTGTTATATTTACCTTTAGGTCGGATCAAGCGCCAAAGTTCTGGTTCATTTGA
GCTGGATCAAAAATTTATTCCACCAATTTTACATATTGAAGCATCAGATACACTGACCTCAAATCTTAAACGGACTCTGA
ATGTCATTCGAGCAAAAATTAAGACCATTCAAACCAATAATCGAGAAAACGAACAGAAACTGATTGAATTCCGTTCTGGT
GATATCGTTTCTTTTTGGCTGGTCAATGCTTTAAACACAGCTCACGCCACACTCAACCATCTGCTTCAACACCCGCAAAT
ACATCCCGAACGACTTTTCTTTGAATTACTCCGTTTGACAGGTTCTTTGCTAACATTTTCTACAGCATATGAAGTAGATC
ATCTTCCTCAATACCGACATCACCAACTACAAGATAGTTTTCAGCAACTTGATGTGATTTTGCGTGATCTGTTAGACACC
ATTATTTCTAGTCGCTATATCAGTATTGCGCTTAAAGAGATTCGTCCATCTTATTGGATCGGAAGCTTGGAATCAGACAA
AATTACGCGAGATAGCAGACTTTATATTGCGGTTTCAAGTAGCATGATGCAGACCCACGAACTGGTTCAAATTGTTCCTT
TACGTTTTAAAGTGGGGAGTACGGTGGATGTTGAACAACGTGTTGTCGCAGCTTTACCTGCAATTCCATTACACCACCTG
ATGCAGGTACCTACCGCGATTCCTGTACGCTCAGGTGTGAGTTATTTTGAAATTGAACCCCATCATGAACTTTATCAACG
TATGCTAGAGTCAGAATCGATATGTATTTATATCCCTTCAGGTTTCCAAGACATTAGCATTGAATTAATTGCTGTAATGA
ATGCCTAA