Detailed information of component protein


Summary

Component ID T6CP000528
Component protein type TssA
T6SS ID (Type) T6SS00061 experimental (Type i4b)
Strain Acinetobacter sp. ADP1
Replicon chromosome
Sequence Protein sequence (368 a.a.); Nucleotide sequence (1104 bp)
Reference
[1] Basler M, Ho BT, Mekalanos JJ. Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions. Cell. 2013 Feb 14;152(4):884-94. doi: 10.1016/j.cell.2013.01.042. PMID: 23415234
[2] Weber BS, Miyata ST, Iwashkiw JA, et al. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One. 2013;8(1):e55142. doi: 10.1371/journal.pone.0055142. Epub 2013 Jan 24. PMID: 23365692
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Shneider MM, Buth SA, Ho BT, et al. PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 2013 Aug 15;500(7462):350-353. doi: 10.1038/nature12453. Epub 2013 Aug 7. PMID: 23925114
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACIAD_RS12255
Coordinate (Strand) 2652825..2653928 (+)
NCBI ID WP_004929021.1
RefSeq NC_005966
Uniprot ID Q6F917_ACIAD
KEGG ID aci:ACIAD2695
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
ImpA_N PF06812.14 2.2e-33 9..131
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-367 MSINISELLQPITEQSPCGEDYSFSNEFHLIKKARTQDDVLLDQGDWVTELKQADWETVTSTSSNLLKTKTKDIRLLTWLSEGWANLYGFEGIYQGIELSHRLLDQFWNDIHPVVEDDDLDQRIALLQGLINQLPLLIKKTPLINTSPYYHLLNYENFLHHQNIRRKQSDEYEQLDGPSEIELFEQALFNTSKSFQYQNYQMFKHVVSQWQQLKDVLDRLMGVDAPSFAAIDSSLQDIDVNLRKIYKAEAFDTNPVATSSATASSHASETLSTEPMPTSNVNVNQPSFQPQTQNHIQNREQAIRVLQDISDYFQANEPHSPVSYMLQKTIKWSQMPLHEWLAQVIKDEHPLQLVQEALGVQPKNEYE


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9919 - -


  Protein sequence: 368 a.a.    

>T6CP000528 NC_005966:2652825-2653928 [Acinetobacter baylyi ADP1] [TssA]
MSINISELLQPITEQSPCGEDYSFSNEFHLIKKARTQDDVLLDQGDWVTELKQADWETVTSTSSNLLKTKTKDIRLLTWL
SEGWANLYGFEGIYQGIELSHRLLDQFWNDIHPVVEDDDLDQRIALLQGLINQLPLLIKKTPLINTSPYYHLLNYENFLH
HQNIRRKQSDEYEQLDGPSEIELFEQALFNTSKSFQYQNYQMFKHVVSQWQQLKDVLDRLMGVDAPSFAAIDSSLQDIDV
NLRKIYKAEAFDTNPVATSSATASSHASETLSTEPMPTSNVNVNQPSFQPQTQNHIQNREQAIRVLQDISDYFQANEPHS
PVSYMLQKTIKWSQMPLHEWLAQVIKDEHPLQLVQEALGVQPKNEYE*

  Nucleotide sequence: 1104 bp    

>T6CP000528 NC_005966:2652825-2653928 [Acinetobacter baylyi ADP1] [TssA]
ATGAGTATAAACATTTCAGAGTTGTTACAGCCAATTACTGAACAATCTCCATGTGGAGAGGATTACTCATTTTCCAATGA
ATTTCATCTGATCAAAAAAGCCAGAACACAAGATGATGTCTTACTTGATCAGGGCGATTGGGTCACTGAATTGAAACAGG
CTGATTGGGAAACAGTGACCAGTACCTCATCAAACCTATTAAAAACAAAAACCAAGGATATTCGCTTACTTACTTGGCTG
AGTGAAGGATGGGCTAATCTATATGGATTTGAAGGTATTTATCAGGGCATTGAATTAAGTCATCGTTTACTGGATCAATT
CTGGAATGACATCCATCCTGTGGTAGAAGATGATGATCTGGATCAAAGAATCGCATTGTTACAGGGCTTAATTAATCAAC
TGCCCTTACTCATCAAGAAAACACCTTTAATTAATACGTCGCCTTATTATCATTTGCTCAATTACGAAAATTTTTTACAT
CATCAGAATATTCGACGTAAGCAAAGCGACGAGTATGAACAGTTAGATGGTCCCTCTGAAATCGAACTATTTGAACAAGC
TTTATTTAATACATCAAAAAGTTTTCAATATCAGAATTATCAAATGTTTAAGCATGTTGTATCTCAATGGCAACAGCTTA
AGGATGTACTGGATCGCCTGATGGGAGTGGATGCTCCAAGTTTCGCAGCAATTGATTCCAGCCTGCAAGATATTGATGTC
AATCTCCGTAAAATTTATAAAGCCGAAGCCTTCGATACAAATCCAGTAGCAACTTCATCGGCTACAGCTTCTAGCCATGC
TTCTGAAACATTGAGTACCGAGCCTATGCCTACCTCAAATGTCAATGTAAACCAGCCGAGCTTTCAACCGCAAACCCAAA
ATCACATTCAAAATCGTGAACAAGCGATTCGGGTATTGCAAGATATTTCTGACTACTTTCAGGCAAATGAGCCTCATAGT
CCCGTCAGTTATATGCTGCAAAAAACGATTAAATGGAGCCAGATGCCATTACATGAATGGTTAGCACAAGTCATTAAAGA
TGAGCATCCGTTACAGTTGGTACAAGAAGCTCTTGGTGTACAACCTAAAAATGAATATGAATAA