Detailed information of component protein


Summary

Component ID T6CP000525
Component protein type TssC
T6SS ID (Type) T6SS00061 experimental (Type i4b)
Strain Acinetobacter sp. ADP1
Replicon chromosome
Sequence Protein sequence (494 a.a.); Nucleotide sequence (1482 bp)
Reference
[1] Basler M, Ho BT, Mekalanos JJ. Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions. Cell. 2013 Feb 14;152(4):884-94. doi: 10.1016/j.cell.2013.01.042. PMID: 23415234
[2] Weber BS, Miyata ST, Iwashkiw JA, et al. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One. 2013;8(1):e55142. doi: 10.1371/journal.pone.0055142. Epub 2013 Jan 24. PMID: 23365692
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Shneider MM, Buth SA, Ho BT, et al. PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 2013 Aug 15;500(7462):350-353. doi: 10.1038/nature12453. Epub 2013 Aug 7. PMID: 23925114
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACIAD_RS12235
Coordinate (Strand) 2647094..2648575 (-)
NCBI ID WP_004929011.1
RefSeq NC_005966
Uniprot ID UPI0002D03137
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
VipB PF05943.14 2.5e-138 66..368
VipB_2 PF18945.2 6e-45 378..488
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-493 MNNPQLANNTALEPENTTLLDSIVEQSRIARNDEEHVRAKNLIGELAKEVMAGTITVSENMTLSIDKRIAEIDALISAQLSQIMHHEKFQKIESTWRGLYYFCQETPSNPLIKIRMLNTTKKELVKDFQGATDFDQSTLFKKIYEEEYGSFGGAPYSALIGDFEFDRTPSDMYLLEQISHVAAAAHAPFISAADPSMFGLESYTDIDRPRDVSKIFETAEYVQWRSFRDSEDARYVALTMPRVLGRLPYHPKEGTATEGFNFIEDVTGQDHNEYLWMNAAYAFGTRLTNAFDLHGWCAAIRGVEGGGLVEGLPVHTFKTHDGEVAFKCPTEIAITDRREKELSDLGFIPLVHCKNTDYAAFFGAQSTQKPKKYDSDTANANSALSSQIQYIMAVSRIAHYLKAMMRDKVGSFASAGNVEAFLNEWLSQYVLLDDGASQEAKAQYPLREASVKVVEDPAQPGHYKSVVFLRPHFQLDELSVSLRLVTELPQSSN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9910 - -


  Protein sequence: 494 a.a.    

>T6CP000525 NC_005966:c2648575-2647094 [Acinetobacter baylyi ADP1] [TssC]
MNNPQLANNTALEPENTTLLDSIVEQSRIARNDEEHVRAKNLIGELAKEVMAGTITVSENMTLSIDKRIAEIDALISAQL
SQIMHHEKFQKIESTWRGLYYFCQETPSNPLIKIRMLNTTKKELVKDFQGATDFDQSTLFKKIYEEEYGSFGGAPYSALI
GDFEFDRTPSDMYLLEQISHVAAAAHAPFISAADPSMFGLESYTDIDRPRDVSKIFETAEYVQWRSFRDSEDARYVALTM
PRVLGRLPYHPKEGTATEGFNFIEDVTGQDHNEYLWMNAAYAFGTRLTNAFDLHGWCAAIRGVEGGGLVEGLPVHTFKTH
DGEVAFKCPTEIAITDRREKELSDLGFIPLVHCKNTDYAAFFGAQSTQKPKKYDSDTANANSALSSQIQYIMAVSRIAHY
LKAMMRDKVGSFASAGNVEAFLNEWLSQYVLLDDGASQEAKAQYPLREASVKVVEDPAQPGHYKSVVFLRPHFQLDELSV
SLRLVTELPQSSN*

  Nucleotide sequence: 1482 bp    

>T6CP000525 NC_005966:c2648575-2647094 [Acinetobacter baylyi ADP1] [TssC]
ATGAATAATCCTCAACTCGCAAATAATACTGCACTTGAACCTGAAAATACGACGTTGCTTGATTCGATTGTTGAGCAGAG
TCGAATTGCCCGAAATGATGAAGAGCATGTTCGGGCTAAGAACCTGATCGGCGAGTTGGCAAAAGAGGTGATGGCTGGAA
CAATTACTGTTTCGGAAAATATGACTTTGTCGATTGATAAGCGTATTGCTGAAATTGATGCTCTTATCTCAGCACAGTTA
AGTCAGATCATGCACCATGAAAAGTTTCAGAAAATTGAATCGACATGGCGCGGCTTATATTATTTTTGTCAGGAAACACC
TTCAAATCCGCTGATTAAAATTCGGATGCTGAACACCACCAAGAAAGAACTGGTCAAAGATTTTCAGGGTGCAACAGATT
TCGATCAGAGCACTTTGTTTAAAAAAATTTATGAAGAAGAATATGGTTCCTTTGGTGGAGCCCCTTATTCTGCACTGATT
GGTGATTTTGAATTTGATCGTACCCCATCAGATATGTATTTGCTTGAGCAAATCTCACATGTTGCTGCTGCTGCTCATGC
GCCGTTTATTTCGGCGGCAGATCCAAGCATGTTTGGTCTGGAAAGTTATACCGATATCGACCGTCCGCGTGACGTCTCCA
AAATTTTTGAAACAGCCGAATATGTGCAGTGGCGATCTTTCCGTGACAGTGAAGATGCACGTTATGTCGCATTGACCATG
CCGCGCGTGCTGGGTCGTTTGCCATATCATCCTAAAGAAGGAACCGCAACAGAAGGTTTCAATTTTATAGAGGATGTGAC
TGGTCAGGATCACAATGAATATCTATGGATGAATGCTGCTTATGCATTTGGTACACGTTTAACCAATGCCTTTGATTTGC
ATGGATGGTGTGCTGCGATTCGCGGTGTCGAAGGTGGTGGTTTAGTTGAAGGCTTACCTGTACATACCTTTAAGACTCAC
GATGGTGAGGTGGCGTTCAAATGTCCAACAGAAATTGCAATTACTGACCGACGTGAAAAAGAACTGAGTGATTTGGGTTT
CATTCCACTCGTTCATTGTAAAAATACCGATTATGCCGCTTTCTTCGGTGCGCAATCGACACAGAAGCCAAAAAAATATG
ACAGTGATACTGCCAATGCCAATTCGGCATTGTCTAGCCAGATTCAATATATTATGGCGGTATCCAGAATTGCACATTAT
TTAAAAGCAATGATGCGCGATAAAGTCGGAAGTTTCGCTTCAGCAGGGAATGTCGAAGCATTCTTGAATGAATGGTTGTC
TCAATATGTATTGCTTGATGATGGTGCTTCACAGGAAGCCAAAGCCCAGTATCCACTTCGTGAGGCATCCGTAAAGGTTG
TAGAAGACCCAGCACAGCCTGGTCACTACAAATCTGTGGTCTTCTTAAGACCGCATTTCCAGCTGGATGAGTTGTCAGTT
TCTTTAAGACTGGTGACGGAACTTCCGCAGTCCTCTAACTAG