Detailed information of component protein


Summary

Component ID T6CP000522
Component protein type TssF
T6SS ID (Type) T6SS00061 experimental (Type i4b)
Strain Acinetobacter sp. ADP1
Replicon chromosome
Sequence Protein sequence (603 a.a.); Nucleotide sequence (1809 bp)
Reference
[1] Basler M, Ho BT, Mekalanos JJ. Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions. Cell. 2013 Feb 14;152(4):884-94. doi: 10.1016/j.cell.2013.01.042. PMID: 23415234
[2] Weber BS, Miyata ST, Iwashkiw JA, et al. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One. 2013;8(1):e55142. doi: 10.1371/journal.pone.0055142. Epub 2013 Jan 24. PMID: 23365692
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Shneider MM, Buth SA, Ho BT, et al. PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 2013 Aug 15;500(7462):350-353. doi: 10.1038/nature12453. Epub 2013 Aug 7. PMID: 23925114
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACIAD_RS12220
Coordinate (Strand) 2644182..2645990 (-)
NCBI ID WP_004929001.1
RefSeq NC_005966
Uniprot ID Q6F924_ACIAD
KEGG ID aci:ACIAD2687
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_TssF PF05947.14 5.4e-153 3..598
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-602 VIEELLPYYEKQLQEFSQQSREFALKYPKIAQRLALNQEQIDDPHIERLIQAFSLIAARIDKKIADSYDFFTRSLFEVMFPQYLRHFPACSVVSFEDLQKLKQLTEPHIIPKQTKLKSRSFKGVQCEFNTAYEVRLLPISLVKLEFKTLQHTHAHLTQNASLNFHFSLLNPLQSWLYQEKLPIYLDAISNFPLQLLDQIFKKETRFSLRVGHQSFDINNPFEMMGFSEAEALLPLDQHTHHAYRLLMEYFCFPEKFNYLNLNLSQLKAFITTQTEFELLIHLKLNLNDPASVRNYSELDVENFKLFTTPVINLFEKQAEPQKINHQQLEYPLITDAHYPDAYQVYSIIDMQMIREKTHQEQAIVPVLPFFAMSHYHDHQVKFFYALHQQKLTKHHVETRFSIVSKQLEPYNTTSDFVSTQLLCSNRDLPYEVLGQTHTSLNLNDSNLARRAVVLKRPTLPYQFEQHKNEQWRIISHLSLNTLALMKGESLSHLKELLALYNLPRSKENHLLIDAIKQLNFSLTHKLVEAKPFPMFVRGVKAEISIDTSVFRGHSVYIFSQLVSHILNLKVQMNSFVDVVIRDSVRQQELYQCVQNVGGKTLL


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9965 - -


  Protein sequence: 603 a.a.    

>T6CP000522 NC_005966:c2645990-2644182 [Acinetobacter baylyi ADP1] [TssF]
VIEELLPYYEKQLQEFSQQSREFALKYPKIAQRLALNQEQIDDPHIERLIQAFSLIAARIDKKIADSYDFFTRSLFEVMF
PQYLRHFPACSVVSFEDLQKLKQLTEPHIIPKQTKLKSRSFKGVQCEFNTAYEVRLLPISLVKLEFKTLQHTHAHLTQNA
SLNFHFSLLNPLQSWLYQEKLPIYLDAISNFPLQLLDQIFKKETRFSLRVGHQSFDINNPFEMMGFSEAEALLPLDQHTH
HAYRLLMEYFCFPEKFNYLNLNLSQLKAFITTQTEFELLIHLKLNLNDPASVRNYSELDVENFKLFTTPVINLFEKQAEP
QKINHQQLEYPLITDAHYPDAYQVYSIIDMQMIREKTHQEQAIVPVLPFFAMSHYHDHQVKFFYALHQQKLTKHHVETRF
SIVSKQLEPYNTTSDFVSTQLLCSNRDLPYEVLGQTHTSLNLNDSNLARRAVVLKRPTLPYQFEQHKNEQWRIISHLSLN
TLALMKGESLSHLKELLALYNLPRSKENHLLIDAIKQLNFSLTHKLVEAKPFPMFVRGVKAEISIDTSVFRGHSVYIFSQ
LVSHILNLKVQMNSFVDVVIRDSVRQQELYQCVQNVGGKTLL*

  Nucleotide sequence: 1809 bp    

>T6CP000522 NC_005966:c2645990-2644182 [Acinetobacter baylyi ADP1] [TssF]
GTGATCGAAGAACTTTTGCCTTATTATGAAAAACAGCTTCAAGAGTTTAGTCAGCAATCACGTGAATTTGCGCTGAAATA
TCCTAAAATTGCACAGCGTTTGGCTTTAAATCAGGAACAAATTGACGATCCACATATTGAGCGTCTAATTCAGGCCTTTT
CATTAATTGCAGCCCGAATCGATAAAAAAATTGCTGATAGTTATGATTTTTTTACACGTTCTCTATTTGAGGTGATGTTT
CCACAGTATTTAAGACATTTTCCTGCGTGTAGTGTGGTCAGTTTTGAAGATTTGCAAAAATTAAAACAATTGACAGAGCC
ACATATTATTCCAAAGCAAACGAAACTTAAATCAAGAAGCTTTAAAGGCGTTCAATGTGAGTTTAATACGGCTTATGAGG
TTCGATTATTGCCGATTAGTCTGGTCAAACTTGAATTTAAGACCTTACAACATACGCATGCACATCTCACACAAAATGCG
AGCCTGAATTTTCATTTTAGCTTACTCAATCCTTTACAATCTTGGTTATATCAAGAAAAATTACCTATCTATTTAGATGC
GATCTCTAATTTTCCTTTACAACTTCTTGATCAAATTTTTAAAAAAGAAACTCGTTTTTCTCTGCGTGTAGGGCATCAAT
CTTTTGATATTAATAATCCATTTGAAATGATGGGTTTTTCAGAAGCTGAGGCATTACTCCCACTTGACCAGCATACCCAT
CATGCTTATCGCTTATTGATGGAGTATTTTTGTTTCCCTGAAAAATTCAATTATTTGAATTTGAATTTATCACAGCTTAA
GGCATTCATCACTACCCAAACTGAGTTTGAGCTTTTAATTCATCTAAAACTTAATCTGAATGATCCTGCAAGTGTGCGTA
ATTATTCAGAACTTGATGTCGAAAATTTTAAGCTTTTTACTACACCAGTGATTAATTTATTTGAAAAGCAGGCCGAACCG
CAAAAAATCAATCATCAACAGCTTGAATATCCGTTAATCACAGATGCACATTATCCTGATGCTTATCAGGTGTATTCCAT
CATTGATATGCAAATGATTCGTGAAAAAACACATCAAGAGCAAGCGATTGTTCCTGTTTTACCCTTTTTTGCAATGAGTC
ACTATCATGATCATCAGGTTAAGTTTTTCTATGCATTACATCAGCAGAAGCTGACTAAACATCATGTAGAAACTCGTTTT
TCCATTGTATCTAAACAGCTTGAACCCTATAACACCACTTCTGATTTTGTAAGTACTCAATTACTTTGTTCTAATCGGGA
TTTACCGTATGAAGTGCTTGGGCAGACTCACACTTCGCTCAATTTAAATGACAGTAATCTTGCGCGCCGCGCTGTAGTGT
TAAAGAGACCAACACTGCCTTATCAGTTTGAGCAACATAAAAATGAGCAGTGGAGAATTATCTCCCATCTTTCATTAAAT
ACTTTGGCACTGATGAAAGGGGAAAGTTTGTCTCATTTAAAAGAGCTGTTGGCTTTATATAATTTGCCACGTTCTAAAGA
GAATCATTTATTGATTGATGCGATCAAACAATTGAATTTTAGTCTGACGCACAAGCTAGTTGAAGCCAAACCGTTTCCAA
TGTTTGTTCGTGGGGTCAAGGCCGAGATAAGTATCGATACATCAGTCTTTAGAGGTCATAGTGTTTATATTTTTAGTCAA
TTAGTGAGTCACATTTTAAATCTTAAAGTTCAAATGAACAGTTTTGTAGATGTGGTGATTCGGGATAGTGTTCGTCAACA
GGAGTTATACCAATGCGTACAGAACGTTGGTGGCAAGACACTTCTGTAA