Detailed information of component protein


Summary

Component ID T6CP000520
Component protein type TssM
T6SS ID (Type) T6SS00061 experimental (Type i4b)
Strain Acinetobacter sp. ADP1
Replicon chromosome
Sequence Protein sequence (1274 a.a.); Nucleotide sequence (3822 bp)
Reference
[1] Basler M, Ho BT, Mekalanos JJ. Tit-for-tat: type VI secretion system counterattack during bacterial cell-cell interactions. Cell. 2013 Feb 14;152(4):884-94. doi: 10.1016/j.cell.2013.01.042. PMID: 23415234
[2] Weber BS, Miyata ST, Iwashkiw JA, et al. Genomic and functional analysis of the type VI secretion system in Acinetobacter. PLoS One. 2013;8(1):e55142. doi: 10.1371/journal.pone.0055142. Epub 2013 Jan 24. PMID: 23365692
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Shneider MM, Buth SA, Ho BT, et al. PAAR-repeat proteins sharpen and diversify the type VI secretion system spike. Nature. 2013 Aug 15;500(7462):350-353. doi: 10.1038/nature12453. Epub 2013 Aug 7. PMID: 23925114
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) ACIAD_RS12205
Coordinate (Strand) 2637964..2641785 (-)
NCBI ID WP_004928993.1
RefSeq NC_005966
Uniprot ID Q6F927_ACIAD
KEGG ID aci:ACIAD2684
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.8e-83 497..838
IcmF-related_N PF14331.8 1.5e-81 188..448
IcmF_C PF06744.14 2e-18 1108..1199
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-4 MYTI
Transmembrane helix 5-27 LGYLWQYITNPKAIIALSLLVAL
Outside 28-36 ISAYSSIPR
Transmembrane helix 37-59 HIFWILVALYLLGILAYGIYWLI
Inside 60-444 QRRRHAQQGEQLAQAIEKDIEVESGLHKNKEELSLIQQQMKESVQLIRKSRLGDKKGNAALYELPWYMVIGNPAAGKSSAIYNSGLKFPFEESHQKMVSAGLSGTRNCDWFFSTDGVLLDTAGRYSVYSEDHSEWLGFLNLLKKNRPKAPVNGLIVIVSIAELISQSPESSLKLAKNLRARIQDLTEKLEVIVPVYLVFSKMDLIAGFTEFFECYEAQEYNQAWGATLPYEQNSSQNAVDLFEQHYTILYEGLKGVSTTHLSRRHAQNISPSVMTFPLEFKTLKPALRSFVSTLFEDNPYQFQPVFRGFYFTSALQEGIVESPMTEQIAQEFHLHPIENSDQQNQHRAHGQNHGYFLKGLFSDVILKDKELVKQHINPAKKRKRH
Transmembrane helix 445-464 IAFIGSLVGVSIILGVWVWS
Outside 465-1273 YRNNQQLIQDVQADLNKVVQLEKVSGQQLSTQLDALLILQERLQQLDEFDEHRPLKFSFGLYQGNHLREQLKTEYLKGIRQIVLTPTQQNIAQYLQRVKGNEETLKANHSNVEIKQVAKTQQYLEPSDSNPQDAYNALKAYLMMSNPQYRESSHLSDQVTRFWRSWLDANKGQMPRGEMIQKAEQILSYAMTLSSDTQFPVLQADSQLVDQTRQVLLSVIRGMPARDRVYNEIKMRAAVRYPAVTVTQIVGESSKGIILGSYALPGMFTHKAWTDYVDQAIEDAANKPTDSKDWVLNSRQSDDLTFSGSPDQIRKQLTALYKQEYIEEWRKFLNGIHYAKAAQFAQQVKNIDALGEPQNSPIRTLLDRVAIETNWDNPVVQAELAAPQKGFVAWFKRKVLNRDDQKLKQQAMAKAQGQISQQYQMFYQLVRKRDDQQNKSLLDEYMESLAQVRSKFNELKNAGEIGPSAMALVKQTINDQNSVFNTTQKIIDEKMSVGVGELDQQLLQRFLGAPLTQSFDSLIIPAQDEMNKLWVMQAYQPFTTNLSQKYPFNSSASLQATSAEIGQIFGENGSIARFVKENLDPLVIRRGYTLTSKTWKDLGISLNPQFVMNFQRYVAPTNGMATGELNQAPAAAPAANQSNFQFYPLQNPQLLSYSIDIDGQRMTYENGIQQWVNFVWPNQGAIPGARITAVDLQGKTHTIFDEPGEYGINRLIDSAQRKEQNGSFEMIWKSKADPNLFVKVNFRLISGNHSGNIGSSRGYAGLQLVDKVTTTKAVRVVAAQPAPASSVGQAQNQPAVTTPPPVGER


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9898 - -


  Protein sequence: 1274 a.a.    

>T6CP000520 NC_005966:c2641785-2637964 [Acinetobacter baylyi ADP1] [TssM]
MYTILGYLWQYITNPKAIIALSLLVALISAYSSIPRHIFWILVALYLLGILAYGIYWLIQRRRHAQQGEQLAQAIEKDIE
VESGLHKNKEELSLIQQQMKESVQLIRKSRLGDKKGNAALYELPWYMVIGNPAAGKSSAIYNSGLKFPFEESHQKMVSAG
LSGTRNCDWFFSTDGVLLDTAGRYSVYSEDHSEWLGFLNLLKKNRPKAPVNGLIVIVSIAELISQSPESSLKLAKNLRAR
IQDLTEKLEVIVPVYLVFSKMDLIAGFTEFFECYEAQEYNQAWGATLPYEQNSSQNAVDLFEQHYTILYEGLKGVSTTHL
SRRHAQNISPSVMTFPLEFKTLKPALRSFVSTLFEDNPYQFQPVFRGFYFTSALQEGIVESPMTEQIAQEFHLHPIENSD
QQNQHRAHGQNHGYFLKGLFSDVILKDKELVKQHINPAKKRKRHIAFIGSLVGVSIILGVWVWSYRNNQQLIQDVQADLN
KVVQLEKVSGQQLSTQLDALLILQERLQQLDEFDEHRPLKFSFGLYQGNHLREQLKTEYLKGIRQIVLTPTQQNIAQYLQ
RVKGNEETLKANHSNVEIKQVAKTQQYLEPSDSNPQDAYNALKAYLMMSNPQYRESSHLSDQVTRFWRSWLDANKGQMPR
GEMIQKAEQILSYAMTLSSDTQFPVLQADSQLVDQTRQVLLSVIRGMPARDRVYNEIKMRAAVRYPAVTVTQIVGESSKG
IILGSYALPGMFTHKAWTDYVDQAIEDAANKPTDSKDWVLNSRQSDDLTFSGSPDQIRKQLTALYKQEYIEEWRKFLNGI
HYAKAAQFAQQVKNIDALGEPQNSPIRTLLDRVAIETNWDNPVVQAELAAPQKGFVAWFKRKVLNRDDQKLKQQAMAKAQ
GQISQQYQMFYQLVRKRDDQQNKSLLDEYMESLAQVRSKFNELKNAGEIGPSAMALVKQTINDQNSVFNTTQKIIDEKMS
VGVGELDQQLLQRFLGAPLTQSFDSLIIPAQDEMNKLWVMQAYQPFTTNLSQKYPFNSSASLQATSAEIGQIFGENGSIA
RFVKENLDPLVIRRGYTLTSKTWKDLGISLNPQFVMNFQRYVAPTNGMATGELNQAPAAAPAANQSNFQFYPLQNPQLLS
YSIDIDGQRMTYENGIQQWVNFVWPNQGAIPGARITAVDLQGKTHTIFDEPGEYGINRLIDSAQRKEQNGSFEMIWKSKA
DPNLFVKVNFRLISGNHSGNIGSSRGYAGLQLVDKVTTTKAVRVVAAQPAPASSVGQAQNQPAVTTPPPVGER*

  Nucleotide sequence: 3822 bp    

>T6CP000520 NC_005966:c2641785-2637964 [Acinetobacter baylyi ADP1] [TssM]
ATGTATACAATTTTAGGCTACTTGTGGCAGTACATCACCAATCCTAAAGCAATCATTGCATTGTCGTTGCTGGTTGCGCT
GATTTCAGCATACTCGTCTATTCCTCGACATATTTTCTGGATATTGGTGGCATTATATCTGTTGGGGATTTTAGCCTATG
GTATTTATTGGCTCATTCAGCGACGTCGTCATGCACAGCAAGGTGAGCAACTTGCACAAGCCATAGAAAAAGATATTGAA
GTAGAAAGTGGACTTCATAAAAATAAAGAAGAGCTCAGTTTAATCCAGCAGCAAATGAAAGAATCTGTGCAGCTCATCCG
TAAATCTCGTCTTGGAGATAAAAAGGGCAATGCTGCACTTTACGAATTGCCTTGGTATATGGTGATTGGAAATCCAGCGG
CAGGAAAAAGCTCTGCAATCTACAATTCGGGCTTAAAGTTTCCATTTGAAGAAAGTCATCAAAAAATGGTTTCTGCTGGG
TTAAGTGGTACGCGTAACTGTGACTGGTTTTTTTCAACAGATGGTGTATTGCTGGATACCGCAGGTCGATATTCTGTTTA
CTCAGAAGATCATTCTGAATGGCTCGGATTCTTGAACTTACTCAAAAAGAATCGACCAAAAGCACCTGTGAATGGTTTGA
TTGTTATTGTGAGCATCGCGGAATTGATTAGTCAAAGTCCAGAAAGCTCCTTAAAACTTGCCAAAAATCTGCGCGCCCGT
ATTCAGGATCTCACTGAAAAACTGGAAGTGATTGTTCCTGTGTATTTGGTATTCTCCAAAATGGACTTGATTGCAGGCTT
TACCGAGTTTTTCGAATGCTACGAAGCACAAGAATACAATCAGGCATGGGGAGCCACCTTGCCTTATGAGCAAAATTCCT
CGCAAAATGCCGTCGATTTATTTGAGCAACATTACACGATTTTGTATGAAGGGCTTAAAGGGGTTAGTACAACGCATTTG
AGTCGTCGCCATGCTCAAAATATTTCTCCAAGTGTCATGACCTTTCCGCTAGAGTTTAAAACGCTTAAACCAGCATTAAG
AAGTTTTGTTTCAACATTATTTGAAGATAATCCTTATCAGTTCCAGCCTGTATTTAGAGGATTTTATTTCACGAGTGCAT
TACAAGAAGGGATTGTTGAAAGTCCAATGACTGAGCAGATTGCTCAGGAGTTTCATCTACATCCGATTGAAAATTCTGAT
CAGCAAAACCAGCATAGGGCACACGGACAAAATCATGGCTATTTTCTCAAAGGACTGTTTTCTGATGTCATCTTAAAAGA
TAAGGAGTTGGTGAAACAACATATCAATCCTGCCAAAAAACGCAAGCGTCATATTGCCTTTATTGGATCGTTGGTAGGGG
TATCCATCATTTTAGGTGTTTGGGTTTGGTCGTATCGTAATAACCAACAACTGATTCAAGATGTTCAAGCCGATTTGAAT
AAAGTGGTACAACTGGAAAAAGTTTCTGGTCAACAATTATCAACACAGCTTGATGCCTTGCTTATTTTACAAGAGCGCTT
ACAACAGTTAGATGAATTTGATGAACATCGACCACTTAAATTCAGCTTTGGTCTTTATCAGGGCAATCACTTACGTGAAC
AACTCAAGACTGAATATCTTAAGGGTATCCGTCAAATTGTGTTAACACCAACCCAGCAAAATATTGCCCAATACTTGCAA
CGGGTAAAAGGAAATGAAGAAACACTCAAGGCCAATCATTCTAATGTTGAAATTAAGCAAGTTGCCAAGACCCAGCAATA
TCTAGAGCCATCTGATAGTAATCCTCAGGACGCTTATAATGCCCTGAAGGCTTATTTGATGATGAGCAATCCGCAATATC
GTGAATCGAGTCATTTAAGCGATCAGGTGACACGCTTCTGGCGTTCATGGCTGGATGCCAATAAAGGACAAATGCCACGT
GGTGAAATGATTCAAAAAGCAGAGCAGATTTTATCTTATGCGATGACTTTATCATCTGATACCCAATTTCCTGTTTTGCA
GGCAGATAGCCAACTGGTAGATCAAACCCGACAAGTTTTATTGTCGGTGATTCGTGGCATGCCAGCACGAGACCGTGTTT
ATAATGAAATTAAAATGCGTGCTGCGGTCAGATATCCAGCCGTAACGGTCACCCAAATTGTTGGAGAGTCGAGTAAAGGA
ATCATTCTGGGAAGTTATGCTTTACCAGGCATGTTCACGCACAAGGCATGGACAGATTATGTCGATCAGGCTATTGAAGA
TGCAGCAAATAAACCAACCGATAGCAAGGATTGGGTACTCAATAGCCGTCAGTCAGATGATTTAACCTTTTCTGGTAGTC
CAGATCAGATTCGCAAACAATTAACAGCACTCTATAAACAGGAATATATTGAAGAATGGCGCAAGTTCTTAAATGGTATT
CATTATGCCAAAGCTGCTCAGTTTGCCCAGCAGGTTAAAAATATTGATGCATTGGGAGAGCCTCAAAACTCACCAATTCG
AACCTTGCTTGATCGTGTCGCAATAGAAACCAACTGGGACAACCCTGTTGTTCAAGCAGAACTTGCAGCACCTCAAAAAG
GTTTTGTCGCTTGGTTTAAGCGTAAAGTTCTCAATCGGGATGATCAGAAGCTCAAGCAACAAGCGATGGCCAAAGCTCAG
GGACAAATTTCGCAACAGTATCAAATGTTTTATCAGTTGGTACGCAAGCGTGATGACCAGCAAAACAAGTCCTTGCTGGA
TGAATATATGGAAAGTCTGGCACAAGTACGAAGTAAATTTAATGAATTGAAAAATGCAGGTGAAATTGGTCCGAGCGCAA
TGGCACTGGTGAAACAGACTATTAATGATCAGAATTCGGTGTTTAATACAACACAGAAAATTATTGATGAAAAAATGTCT
GTGGGAGTAGGTGAGTTGGATCAGCAATTATTGCAGCGTTTTCTTGGCGCTCCGCTGACTCAGTCATTTGATAGCCTTAT
CATTCCTGCTCAAGATGAAATGAATAAGCTGTGGGTGATGCAGGCTTATCAACCATTTACGACCAATTTAAGCCAAAAGT
ATCCATTTAATTCTTCTGCGAGTTTGCAAGCGACCAGCGCCGAAATTGGACAAATTTTTGGTGAGAATGGCAGTATTGCC
CGATTTGTAAAAGAAAATCTTGATCCTTTGGTGATTCGTCGTGGTTATACCTTAACCTCTAAAACATGGAAAGATCTGGG
AATTAGTTTAAATCCACAATTTGTAATGAACTTTCAGCGTTATGTCGCACCAACCAATGGTATGGCAACGGGAGAGTTAA
ATCAGGCACCAGCAGCTGCACCTGCGGCCAATCAATCCAATTTCCAGTTTTATCCATTACAAAATCCGCAGTTACTTTCA
TATTCCATCGATATTGATGGACAGCGTATGACCTATGAAAACGGCATTCAGCAATGGGTAAATTTTGTCTGGCCAAATCA
GGGTGCAATTCCTGGTGCACGAATCACGGCAGTAGATTTACAAGGCAAAACACATACGATATTTGATGAGCCAGGTGAAT
ACGGAATTAACCGTTTGATTGATAGTGCTCAACGTAAAGAACAAAATGGCAGCTTTGAGATGATCTGGAAAAGCAAGGCG
GATCCGAATCTATTTGTCAAAGTCAATTTCCGTCTGATTAGTGGTAACCATTCAGGCAATATTGGATCAAGTCGTGGTTA
TGCAGGTTTACAACTGGTGGATAAGGTCACTACCACTAAAGCCGTACGTGTGGTTGCTGCACAACCAGCACCAGCATCAT
CTGTGGGTCAGGCGCAGAATCAGCCAGCAGTGACAACACCGCCACCTGTGGGAGAGCGCTAA