SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000464
Component protein type	TssF
T6SS ID (Type)	T6SS00053 (Type Unknown)
Strain	Photorhabdus luminescens subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (589 a.a.); Nucleotide sequence (1767 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS20780
Coordinate (Strand)	4915126..4916892 (-)
NCBI ID	WP_011148306.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.5e-191	1..585

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-588	MDDLILRYYEAEMQYLREAGKEFAQAHPDRAAMLNLSDDRDLDPYVERLLEGFAFLIGRMRQKLDDDLPELTEGLVSLLWPHYLRTIPSLSIVELAPDYQTLPKSEVIAKGFEVISQPVGPSQTRCYYRTTRDIQITPLHITSATLQNEADGRSVIRLRFDCGQLTEWDKVDLARLPLYLKAGRPVSSALHLALTRQTQQIYARLSSQEERSRLKNGCFSPMGFDNDDVLWPKQESAFSGYQLLLEYFTFREKFMFVALNGLDSLEIPTGTPWFELDIVLSELWPYELKFSAENIRLHCVPVINLFRVEADPMHISRLQNEYLLRPMRIQDGHTEIYSVDEVSSSRRGDAQRFVPFSSFRHRGGIMRSSAPERYFHTRVCRGPSGLHDTWLILGGESFETDPELDDETLSLRITGTNGQLPRQALRNTQLEQTLHSAAGTLRVSNLCSPTLPCYPPANDRFHWRILSHLGSNFLSMMDNPEVLRGTLGLYNWTNDEMNCRCLDAIVGVKHALIERFEKGFLLRGVDIEITLNSNGFAGEGDIFLFGELLNRFFSLYTDIHLFNQLTLILQPSGRCLRWQENHNQRIPG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9975	-	-

Protein sequence: 589 a.a.

						>T6CP000464 NC_005126:c4916892-4915126 [Photorhabdus laumondii subsp. laumondii TTO1] [TssF]
MDDLILRYYEAEMQYLREAGKEFAQAHPDRAAMLNLSDDRDLDPYVERLLEGFAFLIGRMRQKLDDDLPELTEGLVSLLW
PHYLRTIPSLSIVELAPDYQTLPKSEVIAKGFEVISQPVGPSQTRCYYRTTRDIQITPLHITSATLQNEADGRSVIRLRF
DCGQLTEWDKVDLARLPLYLKAGRPVSSALHLALTRQTQQIYARLSSQEERSRLKNGCFSPMGFDNDDVLWPKQESAFSG
YQLLLEYFTFREKFMFVALNGLDSLEIPTGTPWFELDIVLSELWPYELKFSAENIRLHCVPVINLFRVEADPMHISRLQN
EYLLRPMRIQDGHTEIYSVDEVSSSRRGDAQRFVPFSSFRHRGGIMRSSAPERYFHTRVCRGPSGLHDTWLILGGESFET
DPELDDETLSLRITGTNGQLPRQALRNTQLEQTLHSAAGTLRVSNLCSPTLPCYPPANDRFHWRILSHLGSNFLSMMDNP
EVLRGTLGLYNWTNDEMNCRCLDAIVGVKHALIERFEKGFLLRGVDIEITLNSNGFAGEGDIFLFGELLNRFFSLYTDIH
LFNQLTLILQPSGRCLRWQENHNQRIPG*					

Nucleotide sequence: 1767 bp

						>T6CP000464 NC_005126:c4916892-4915126 [Photorhabdus laumondii subsp. laumondii TTO1] [TssF]
ATGGATGATTTAATTCTCCGCTATTACGAAGCGGAAATGCAATATCTGCGTGAAGCTGGCAAAGAGTTTGCGCAGGCGCA
CCCGGACCGGGCAGCTATGCTGAATCTGAGTGACGATCGGGATCTTGATCCTTATGTGGAGCGATTGCTTGAAGGCTTCG
CTTTCCTGATAGGGCGGATGCGTCAGAAACTTGATGATGATTTGCCGGAACTGACGGAAGGGTTGGTGAGTTTGCTGTGG
CCGCATTATCTACGCACTATTCCTTCACTCTCCATTGTTGAATTGGCACCTGATTATCAGACCTTGCCAAAAAGTGAAGT
GATCGCCAAAGGGTTTGAAGTGATATCTCAGCCCGTAGGACCAAGCCAGACCCGCTGCTATTACCGGACTACCCGTGATA
TACAGATTACACCGCTGCATATCACCTCTGCGACGTTGCAAAATGAAGCCGACGGCCGTTCAGTGATCCGTTTGCGTTTT
GACTGCGGTCAGTTGACCGAGTGGGATAAAGTGGATTTGGCACGTCTGCCTCTGTATCTCAAAGCGGGCCGTCCGGTGAG
TTCTGCCTTACATCTGGCGTTGACCCGACAGACTCAACAGATTTACGCGCGCTTATCCAGTCAGGAAGAGCGCTCTCGCC
TTAAGAATGGCTGTTTTTCCCCGATGGGGTTCGACAATGATGATGTTCTGTGGCCGAAACAGGAGAGCGCGTTCAGTGGT
TATCAGTTGTTACTGGAATATTTTACTTTCCGTGAAAAATTTATGTTTGTGGCGCTCAATGGCTTGGATTCGTTAGAGAT
TCCTACCGGTACGCCTTGGTTTGAGTTGGATATCGTACTCAGTGAATTGTGGCCCTATGAATTGAAGTTTTCAGCGGAAA
ATATCCGATTGCATTGCGTGCCGGTAATCAATTTATTCCGTGTTGAAGCTGATCCGATGCACATTTCGCGGCTGCAAAAT
GAGTATCTACTGCGCCCGATGCGCATTCAGGATGGTCATACCGAAATCTATTCGGTGGATGAAGTCTCCTCTTCCCGCCG
AGGTGATGCTCAGCGTTTTGTACCGTTTAGCAGCTTTCGTCATCGGGGCGGCATCATGCGTAGTTCCGCACCGGAGCGTT
ATTTTCATACGCGCGTCTGTCGAGGTCCTTCGGGTTTGCACGATACCTGGCTGATCCTCGGTGGAGAATCGTTTGAAACT
GACCCAGAGCTTGATGATGAAACCCTTTCACTGCGTATCACCGGAACCAACGGCCAGTTACCTCGTCAGGCATTACGTAA
TACCCAACTTGAACAGACACTACACTCCGCCGCGGGCACGCTGCGCGTAAGCAATCTCTGTTCGCCGACGTTGCCCTGTT
ATCCCCCAGCGAACGACCGTTTCCACTGGCGGATATTAAGCCATCTCGGTTCTAATTTTCTTAGCATGATGGATAACCCG
GAGGTGCTGCGCGGCACGTTAGGGCTTTATAACTGGACAAATGATGAGATGAACTGCCGCTGTCTGGATGCCATTGTTGG
GGTGAAACACGCTTTGATCGAACGTTTTGAAAAGGGCTTCCTACTCCGGGGCGTTGATATTGAGATTACGCTGAACAGCA
ATGGCTTTGCCGGCGAAGGCGATATTTTCCTGTTCGGTGAGCTGCTTAACCGCTTCTTCTCGCTGTATACCGATATTCAT
CTGTTTAACCAATTGACACTGATCCTTCAACCTTCTGGGAGATGTTTGCGATGGCAAGAAAATCACAATCAGCGTATTCC
CGGTTAA					