SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000460
Component protein type	TssB
T6SS ID (Type)	T6SS00052 (Type i2)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (166 a.a.); Nucleotide sequence (498 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Bernard CS, Brunet YR, Gueguen E, et al. Nooks and crannies in type VI secretion regulation. J Bacteriol. 2010 Aug;192(15):3850-60. doi: 10.1128/JB.00370-10. Epub 2010 May 28. PMID: 20511495

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS16250
Coordinate (Strand)	3863474..3863971 (-)
NCBI ID	WP_011147462.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	3.6e-54	7..157

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-165	MTHSFQNEIPKSRINIKLDLHTGGAQKKVELPLKLLVMGDYSNGQEHLPLGERKKVAINKNNFDSVLAEFSPAINLTVKNTLAGDGSEENIALIFRQIKDFEPEQIARQIPQLRALLSMRHLLRDLRSNLLDNTTFRRELEHILQDQALSDELRAELAALAPKTI

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9956	-	-

Protein sequence: 166 a.a.

						>T6CP000460 NC_005126:c3863971-3863474 [Photorhabdus laumondii subsp. laumondii TTO1] [TssB]
MTHSFQNEIPKSRINIKLDLHTGGAQKKVELPLKLLVMGDYSNGQEHLPLGERKKVAINKNNFDSVLAEFSPAINLTVKN
TLAGDGSEENIALIFRQIKDFEPEQIARQIPQLRALLSMRHLLRDLRSNLLDNTTFRRELEHILQDQALSDELRAELAAL
APKTI*					

Nucleotide sequence: 498 bp

						>T6CP000460 NC_005126:c3863971-3863474 [Photorhabdus laumondii subsp. laumondii TTO1] [TssB]
ATGACCCATAGTTTTCAAAACGAGATCCCTAAATCGCGTATTAATATCAAGTTAGATTTACATACAGGAGGTGCGCAGAA
AAAAGTTGAGCTGCCGCTGAAATTATTGGTCATGGGGGATTATAGCAACGGGCAGGAACATCTCCCTCTTGGGGAAAGAA
AAAAAGTGGCTATCAATAAAAACAATTTTGATAGCGTACTGGCTGAATTCAGTCCCGCGATTAACTTGACGGTCAAAAAT
ACCTTGGCCGGAGATGGCAGCGAGGAGAATATCGCCCTTATTTTTCGGCAAATAAAAGACTTCGAGCCTGAACAAATTGC
TCGTCAAATCCCGCAACTGCGGGCCCTGCTTTCTATGCGCCATCTATTGCGCGATCTGAGATCTAACCTGCTCGATAACA
CCACCTTTCGCCGGGAACTGGAACATATCCTCCAGGATCAAGCGTTAAGCGATGAGTTACGGGCTGAACTGGCCGCACTG
GCACCAAAAACGATTTGA					