SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000456
Component protein type	TssM
T6SS ID (Type)	T6SS00052 (Type i2)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (1124 a.a.); Nucleotide sequence (3372 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Bernard CS, Brunet YR, Gueguen E, et al. Nooks and crannies in type VI secretion regulation. J Bacteriol. 2010 Aug;192(15):3850-60. doi: 10.1128/JB.00370-10. Epub 2010 May 28. PMID: 20511495

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS16165
Coordinate (Strand)	3843641..3847012 (-)
NCBI ID	WP_011147451.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	9.1e-67	401..717
IcmF_C	PF06744.14	7.6e-21	977..1081

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-8	MKVNRHHQ
Transmembrane helix	9-31	YGYFGYIFITLSLVGIFLMLIYL
Outside	32-45	NKTLLEERGLLKSA
Transmembrane helix	46-68	LYAYATVFPLLLIGLFSNAVWWH
Inside	69-352	IRQNKAQKKLRPETERTADRSSQSALVLSDLGDRMRLRYGRFWWYKVRILLVVGEVEQVEAIAPGLTTTHWLEGYRTLLLWGGSLQAEPNTAQLMALRRLRRYRPLNAIVWALTENQSAQPVWMDKALRMLQKQAHQLRWQAPVYLWQVCHSTWSQEGRITQTVGCFFPERCTPDIVATQLQTLVDPLRERGMQQLLEKTAHDFLCRLSANLKQQGIAHWQQVLTPWLAEYGDVVSLRGLMFSLPLKAQTTRLPQIWLPDTAWQGVLEGSRCFHGRRVGMLHTQ
Transmembrane helix	353-375	TVCRGLIVLGLLWGAGMVLSFFT
Outside	376-1123	NRDQLAVVQAAVTDLKVQPENSDAQLMALKDLRNEVDRLQDRAEHGVPWYERFGLSQNQKLLTAVLPDYAQVNNRLIRDKAAAVLHAKLSALVNLPPGSPLRAKWAQAGHDQLKAYLMMAQPEKVDAVFLTRILDENEPERAGIAPAVWLDMAWDLWSFYAENLAAHPEWRITPDKALIKQVRQLLLLQLGKHNAEAALYQQMLQSVEKNYSDLTLRQMTGDTDAARLFTTSHVIPGMFTRQAWEGGIQKAIAEAVASRKEAIDWVLSDNRQAVSASVSPEELKARLTARYFTDFAGVWQDFLNSLRWNKTRNLSDVIDQLTLMADGRQSPLIALMNTLAYQGETGQQGPSLSDSLVKSAKTLLNKKTPRMINQQTAGLHGPMDETFGPLLALMGENQAEGRIKADSTLNLQTFLTRVTDVRLKLQQMNNSDDPPAMANALAKTVFEGKSTDLTDTQGYGRLIAASLGGQWRSFGQTLFVEPLAQAWQGVLKPATANLNSQWQTAIVDNWDRAFAGRYPFAGGDSEISLPMLGQFIRSDSGRIEQFLTRQLGGILHKAGNQWVVDEVNGQGVQINPDFLKAINQLSQLSDLLFADGSQGLRFELRARAVRDVVETDLTIDGQKLRYFNQMESWQSFRWPGETYKPGVMLTWTSVNAGARLFGDYQGNWGLIRWLAQAKAERLDESRYRLIFTAPDGLPLTWILRTELGEGPLALLKLRGFKLPKNIFVVTPGNHTTISAVNDDDLITE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9980	-	-

Protein sequence: 1124 a.a.

						>T6CP000456 NC_005126:c3847012-3843641 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
MKVNRHHQYGYFGYIFITLSLVGIFLMLIYLNKTLLEERGLLKSALYAYATVFPLLLIGLFSNAVWWHIRQNKAQKKLRP
ETERTADRSSQSALVLSDLGDRMRLRYGRFWWYKVRILLVVGEVEQVEAIAPGLTTTHWLEGYRTLLLWGGSLQAEPNTA
QLMALRRLRRYRPLNAIVWALTENQSAQPVWMDKALRMLQKQAHQLRWQAPVYLWQVCHSTWSQEGRITQTVGCFFPERC
TPDIVATQLQTLVDPLRERGMQQLLEKTAHDFLCRLSANLKQQGIAHWQQVLTPWLAEYGDVVSLRGLMFSLPLKAQTTR
LPQIWLPDTAWQGVLEGSRCFHGRRVGMLHTQTVCRGLIVLGLLWGAGMVLSFFTNRDQLAVVQAAVTDLKVQPENSDAQ
LMALKDLRNEVDRLQDRAEHGVPWYERFGLSQNQKLLTAVLPDYAQVNNRLIRDKAAAVLHAKLSALVNLPPGSPLRAKW
AQAGHDQLKAYLMMAQPEKVDAVFLTRILDENEPERAGIAPAVWLDMAWDLWSFYAENLAAHPEWRITPDKALIKQVRQL
LLLQLGKHNAEAALYQQMLQSVEKNYSDLTLRQMTGDTDAARLFTTSHVIPGMFTRQAWEGGIQKAIAEAVASRKEAIDW
VLSDNRQAVSASVSPEELKARLTARYFTDFAGVWQDFLNSLRWNKTRNLSDVIDQLTLMADGRQSPLIALMNTLAYQGET
GQQGPSLSDSLVKSAKTLLNKKTPRMINQQTAGLHGPMDETFGPLLALMGENQAEGRIKADSTLNLQTFLTRVTDVRLKL
QQMNNSDDPPAMANALAKTVFEGKSTDLTDTQGYGRLIAASLGGQWRSFGQTLFVEPLAQAWQGVLKPATANLNSQWQTA
IVDNWDRAFAGRYPFAGGDSEISLPMLGQFIRSDSGRIEQFLTRQLGGILHKAGNQWVVDEVNGQGVQINPDFLKAINQL
SQLSDLLFADGSQGLRFELRARAVRDVVETDLTIDGQKLRYFNQMESWQSFRWPGETYKPGVMLTWTSVNAGARLFGDYQ
GNWGLIRWLAQAKAERLDESRYRLIFTAPDGLPLTWILRTELGEGPLALLKLRGFKLPKNIFVVTPGNHTTISAVNDDDL
ITE*					

Nucleotide sequence: 3372 bp

						>T6CP000456 NC_005126:c3847012-3843641 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
ATGAAAGTTAATCGTCACCACCAATATGGCTATTTTGGATATATTTTTATTACCCTTAGCCTTGTCGGGATATTTCTCAT
GTTGATCTACTTGAATAAAACATTACTTGAGGAACGGGGGCTGCTTAAATCCGCATTGTATGCCTACGCCACAGTTTTTC
CGCTCTTATTAATAGGCTTATTTAGCAATGCTGTCTGGTGGCATATTCGGCAGAATAAAGCGCAGAAAAAACTCAGACCT
GAAACCGAGCGGACAGCGGATCGCTCCAGTCAGTCTGCTTTGGTACTGAGTGACTTGGGCGATCGCATGCGCCTCCGTTA
CGGGCGGTTCTGGTGGTATAAAGTCCGCATTTTACTGGTGGTGGGCGAAGTGGAGCAGGTGGAAGCCATCGCGCCGGGGC
TGACCACGACGCACTGGCTGGAGGGTTATCGCACGCTATTGCTCTGGGGCGGCAGTTTGCAAGCGGAACCGAATACCGCT
CAATTGATGGCCCTGCGTCGCTTGCGCCGTTACCGCCCGCTCAATGCCATTGTCTGGGCGCTGACCGAAAACCAAAGTGC
GCAGCCGGTATGGATGGATAAGGCCCTGAGGATGTTGCAAAAACAGGCCCACCAGTTGCGCTGGCAGGCTCCGGTCTACC
TGTGGCAGGTGTGTCACAGTACCTGGTCTCAGGAAGGTCGTATCACTCAGACCGTCGGCTGCTTTTTTCCTGAACGCTGT
ACCCCGGATATCGTGGCGACACAGTTACAAACGTTGGTTGACCCGTTGCGCGAGCGCGGGATGCAGCAACTGTTGGAGAA
GACGGCCCACGATTTTCTCTGTCGTCTGTCCGCGAACTTGAAACAACAGGGGATTGCGCACTGGCAACAGGTCCTGACGC
CGTGGTTAGCGGAGTATGGTGATGTGGTGTCGCTGCGGGGTTTGATGTTCAGTTTGCCGCTTAAAGCGCAGACGACTCGC
CTCCCGCAGATCTGGCTGCCCGACACGGCATGGCAGGGGGTGTTGGAGGGCAGTCGTTGTTTCCACGGTCGGCGGGTGGG
CATGCTCCACACACAGACCGTTTGTCGTGGCCTGATAGTGCTGGGCTTGTTATGGGGTGCCGGAATGGTGCTCTCCTTTT
TCACCAACCGTGACCAGCTAGCCGTGGTGCAGGCGGCGGTAACTGACCTGAAAGTCCAGCCGGAGAATTCTGATGCCCAG
TTGATGGCCTTGAAGGATCTGCGTAACGAGGTGGACCGGCTGCAAGACCGGGCTGAACACGGCGTGCCGTGGTATGAGCG
CTTTGGTTTGAGTCAGAATCAGAAACTGTTGACGGCGGTGCTGCCCGATTATGCGCAGGTTAATAACCGCCTGATACGGG
ACAAAGCCGCTGCGGTGTTGCATGCGAAACTGAGTGCGCTGGTGAATTTACCGCCGGGCAGCCCTTTGCGGGCTAAGTGG
GCGCAAGCCGGTCATGACCAGTTGAAAGCCTACCTGATGATGGCGCAGCCGGAAAAAGTGGATGCTGTGTTTTTAACCCG
GATACTGGATGAAAATGAACCCGAGCGGGCCGGTATTGCCCCCGCTGTCTGGCTGGATATGGCGTGGGATTTGTGGAGTT
TTTATGCGGAGAATCTGGCCGCGCATCCCGAATGGCGGATAACCCCGGATAAGGCGTTGATTAAACAGGTGCGTCAGTTG
TTGCTGCTCCAGCTAGGTAAGCACAATGCCGAAGCGGCGCTTTACCAGCAGATGCTGCAATCAGTGGAGAAAAATTATTC
GGACCTGACGTTAAGGCAGATGACCGGCGACACCGATGCGGCGCGGCTGTTTACCACCTCTCACGTGATACCGGGGATGT
TTACCCGTCAGGCGTGGGAAGGCGGGATACAAAAGGCGATAGCGGAGGCGGTGGCGTCACGTAAAGAGGCAATTGACTGG
GTGCTGAGTGATAACCGTCAGGCAGTCTCGGCTTCGGTATCGCCCGAAGAGCTGAAAGCCCGTTTGACCGCCCGTTACTT
TACCGATTTTGCCGGGGTGTGGCAGGATTTTCTCAACAGCCTGCGCTGGAACAAAACCCGTAACCTGTCGGATGTCATCG
ATCAGTTAACCCTGATGGCGGATGGACGGCAGTCTCCGCTGATTGCGTTGATGAACACACTGGCCTATCAGGGCGAGACC
GGGCAACAGGGGCCGAGTTTATCGGACTCGTTGGTGAAATCCGCTAAAACGTTATTGAATAAAAAAACGCCGCGAATGAT
TAATCAACAGACGGCTGGCTTGCACGGGCCGATGGATGAAACCTTTGGGCCGTTGCTGGCGCTGATGGGAGAAAATCAGG
CAGAAGGAAGAATCAAGGCGGACAGTACCCTGAATTTACAAACCTTCCTGACGCGGGTAACCGATGTGCGTCTGAAACTG
CAACAGATGAACAACAGCGACGACCCACCGGCAATGGCGAATGCGCTGGCTAAAACGGTATTTGAAGGGAAAAGCACTGA
TTTGACCGATACTCAGGGTTATGGGCGCTTGATAGCCGCCAGTCTGGGCGGCCAATGGCGGAGTTTTGGTCAGACGCTGT
TTGTCGAGCCATTAGCGCAGGCGTGGCAGGGAGTATTGAAACCGGCGACGGCGAATCTGAATAGCCAGTGGCAGACGGCG
ATAGTGGACAACTGGGATCGGGCATTTGCGGGCCGTTATCCGTTTGCCGGTGGCGACAGTGAAATTTCATTGCCGATGTT
GGGGCAATTTATCCGGTCAGATTCAGGGCGGATAGAGCAGTTTTTGACGCGTCAGTTAGGCGGGATACTGCATAAAGCGG
GTAACCAGTGGGTAGTGGATGAGGTGAATGGTCAGGGCGTACAGATTAACCCGGATTTTCTAAAGGCGATAAATCAGCTC
AGTCAGTTATCGGACCTGTTGTTTGCGGATGGCAGTCAGGGATTGCGGTTTGAACTGCGGGCCAGAGCGGTGCGGGATGT
GGTGGAAACTGACCTGACGATAGATGGGCAGAAACTGCGTTATTTTAACCAGATGGAAAGTTGGCAGAGCTTTCGCTGGC
CGGGAGAGACCTATAAACCGGGTGTGATGCTGACGTGGACCAGTGTCAATGCCGGGGCGAGATTGTTTGGTGATTATCAG
GGGAACTGGGGGCTGATCCGCTGGCTGGCACAGGCGAAAGCGGAGAGGCTGGATGAAAGCCGTTACCGACTAATATTTAC
CGCGCCGGATGGATTACCGTTAACCTGGATTTTGCGCACAGAATTGGGCGAAGGGCCGCTGGCGTTATTAAAACTGCGCG
GATTTAAATTGCCGAAAAATATCTTTGTAGTGACGCCGGGTAATCACACGACAATATCAGCGGTAAATGACGATGATCTG
ATAACGGAATAG					