SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000454
Component protein type	TssM
T6SS ID (Type)	T6SS00052 (Type i2)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (1129 a.a.); Nucleotide sequence (3387 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Bernard CS, Brunet YR, Gueguen E, et al. Nooks and crannies in type VI secretion regulation. J Bacteriol. 2010 Aug;192(15):3850-60. doi: 10.1128/JB.00370-10. Epub 2010 May 28. PMID: 20511495

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS16120
Coordinate (Strand)	3830821..3834207 (-)
NCBI ID	WP_165828660.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	8.3e-69	406..722
IcmF_C	PF06744.14	5.3e-21	982..1086

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-16	MALQENKIPPISVVKT
Transmembrane helix	17-39	LILLIGTITLLVSLAGLGWWVWG
Outside	40-53	EHRTTDAETFNHLL
Transmembrane helix	54-76	WVCGIAWVLTVVVSMLGFITVTL
Inside	77-357	FVKNKVTDVKSDTRPLLPASEPYDYGDLGDRMRLRYGRFWWYKVRILLVVGEVEQVEAIAPGLTTTHWQEGYRTLLLWGGSLQAEPNTAQLMALRRLRRYRPLNAIVWALTENQSAQPVWMDKALRMLQKQAHQLRWQAPVYLWQVCHSTWSQEGRITQTVGCFFPERCTPDIVATQLQTLVDPLRERGMQQLLEKTAHDFLCRLSANLKQQGIAHWQQVLTPWLAEYGDVVSLRGLMFSLPLKAQTTRLPQIWLPDTAWQGVLEGSRCFHGRRVGMLHTQ
Transmembrane helix	358-380	TVCRGLIVLGLLWGAGMMLSFFT
Outside	381-1128	NRDQLAVVQAAVTDLKAQPENSDAQLMALKALRNEVDRLQDRAEHGVPWYERFGLSQNQKLLTAVVPDYAQVNNRLIRDKAAAVLHAKLSALVNLPPGSPLRAKRAKAGHDQLKAYLMMAQPEKVDAAFLTRILDENEPERAGIAPAVWLDTAWDLWSFYAENLAAHPEWRITPDKALIKQVRQLLLIQPGKHNAEAALYQQMLQSVEKNYSDLTLRQMTGDTDAARLFTTSHVIPGMFTRQAWEGGIQKAIAEAVASRKEAIDWVLSDNRQAVSASVSPEELKARLTARYFTDFAGAWQDFLNSLRWNKTRNLSDVIDQLTLMADGRQSPLIALMNTLAYQGETGQQGPSLSDSLVKSAKTLLNKKTPRMINQQTAGLHGPMDETFGPLLALMGENQAEGRIKADSTLNLQTFLTRVTDVRLKLQQMNNSDDPPAMANALAKTVFEGKSTDLTDTQGYGRLIAASLGGQWRGFGQTLFVEPLAQAWQGVLKPATANLNSQWQTAIVDNWDRAFAGRYPFAGGESEISLPMLGQFIRSDSGRIEQFLTRQLGGILHKAGNQWVVDEVNGQGVQINPDFLKAINQLSQLSDLLFADGSQGLRFELRARAVRDVVETDLTIDGQKLRYFNQMESWQSFRWPGETYKPGVMLTWTSVNAGARLFGDYQGNWGLIRWLAQAKAEKLDESRYRLIFTAPDGLPLTWILRTELGEGPLALLKLRGFKLPKNIFVVTPGNHTTISAVNDDDLIEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9986	-	-

Protein sequence: 1129 a.a.

						>T6CP000454 NC_005126:c3834207-3830821 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
MALQENKIPPISVVKTLILLIGTITLLVSLAGLGWWVWGEHRTTDAETFNHLLWVCGIAWVLTVVVSMLGFITVTLFVKN
KVTDVKSDTRPLLPASEPYDYGDLGDRMRLRYGRFWWYKVRILLVVGEVEQVEAIAPGLTTTHWQEGYRTLLLWGGSLQA
EPNTAQLMALRRLRRYRPLNAIVWALTENQSAQPVWMDKALRMLQKQAHQLRWQAPVYLWQVCHSTWSQEGRITQTVGCF
FPERCTPDIVATQLQTLVDPLRERGMQQLLEKTAHDFLCRLSANLKQQGIAHWQQVLTPWLAEYGDVVSLRGLMFSLPLK
AQTTRLPQIWLPDTAWQGVLEGSRCFHGRRVGMLHTQTVCRGLIVLGLLWGAGMMLSFFTNRDQLAVVQAAVTDLKAQPE
NSDAQLMALKALRNEVDRLQDRAEHGVPWYERFGLSQNQKLLTAVVPDYAQVNNRLIRDKAAAVLHAKLSALVNLPPGSP
LRAKRAKAGHDQLKAYLMMAQPEKVDAAFLTRILDENEPERAGIAPAVWLDTAWDLWSFYAENLAAHPEWRITPDKALIK
QVRQLLLIQPGKHNAEAALYQQMLQSVEKNYSDLTLRQMTGDTDAARLFTTSHVIPGMFTRQAWEGGIQKAIAEAVASRK
EAIDWVLSDNRQAVSASVSPEELKARLTARYFTDFAGAWQDFLNSLRWNKTRNLSDVIDQLTLMADGRQSPLIALMNTLA
YQGETGQQGPSLSDSLVKSAKTLLNKKTPRMINQQTAGLHGPMDETFGPLLALMGENQAEGRIKADSTLNLQTFLTRVTD
VRLKLQQMNNSDDPPAMANALAKTVFEGKSTDLTDTQGYGRLIAASLGGQWRGFGQTLFVEPLAQAWQGVLKPATANLNS
QWQTAIVDNWDRAFAGRYPFAGGESEISLPMLGQFIRSDSGRIEQFLTRQLGGILHKAGNQWVVDEVNGQGVQINPDFLK
AINQLSQLSDLLFADGSQGLRFELRARAVRDVVETDLTIDGQKLRYFNQMESWQSFRWPGETYKPGVMLTWTSVNAGARL
FGDYQGNWGLIRWLAQAKAEKLDESRYRLIFTAPDGLPLTWILRTELGEGPLALLKLRGFKLPKNIFVVTPGNHTTISAV
NDDDLIEE*					

Nucleotide sequence: 3387 bp

						>T6CP000454 NC_005126:c3834207-3830821 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
ATGGCACTGCAAGAGAATAAAATACCGCCTATTTCGGTGGTGAAGACACTTATCTTACTGATAGGCACCATTACCCTGCT
GGTTTCGTTGGCTGGTCTGGGATGGTGGGTTTGGGGAGAACACCGAACCACTGATGCCGAAACTTTCAACCATCTGTTGT
GGGTTTGTGGAATAGCTTGGGTGCTCACTGTGGTGGTGAGTATGCTAGGTTTTATCACGGTAACGTTGTTTGTCAAAAAC
AAAGTCACCGATGTGAAGTCCGATACCCGGCCACTTTTGCCAGCCAGCGAACCGTATGATTATGGTGATTTGGGCGATCG
CATGCGCCTCCGTTACGGGCGGTTCTGGTGGTATAAAGTCCGCATTTTACTGGTGGTGGGCGAAGTGGAACAGGTGGAAG
CCATCGCGCCGGGGCTGACCACGACGCACTGGCAGGAGGGTTATCGCACGCTGTTGCTCTGGGGCGGCAGTTTGCAAGCG
GAACCGAATACCGCTCAATTGATGGCCCTGCGTCGCTTGCGCCGTTACCGCCCGCTCAATGCCATTGTCTGGGCGCTGAC
CGAAAACCAAAGTGCGCAGCCGGTGTGGATGGATAAAGCTTTGCGGATGTTACAAAAACAGGCCCACCAGTTGCGCTGGC
AGGCCCCGGTCTACCTGTGGCAGGTGTGTCACAGTACCTGGTCTCAGGAAGGTCGTATCACTCAGACCGTCGGCTGCTTT
TTTCCTGAACGCTGTACCCCGGATATCGTGGCGACACAGTTACAAACGTTGGTTGACCCGTTGCGCGAGCGCGGGATGCA
GCAACTGTTGGAGAAGACGGCCCACGATTTTCTTTGTCGTCTGTCCGCGAACTTGAAACAACAGGGGATTGCGCACTGGC
AACAGGTCCTGACGCCGTGGTTAGCGGAGTATGGTGATGTGGTGTCGCTGCGGGGTTTGATGTTCAGTTTGCCGCTTAAA
GCGCAGACGACTCGCCTCCCGCAGATCTGGCTGCCCGACACGGCATGGCAGGGGGTGTTGGAGGGCAGTCGTTGTTTCCA
CGGTCGGCGGGTGGGCATGCTCCACACACAGACCGTTTGTCGTGGCCTGATAGTGCTGGGCTTGTTATGGGGGGCCGGAA
TGATGCTCTCCTTTTTCACCAACCGTGACCAGCTAGCTGTGGTGCAGGCGGCGGTCACTGACCTGAAAGCTCAGCCGGAG
AATTCTGATGCCCAGTTGATGGCCTTAAAGGCGCTGCGTAACGAGGTGGACCGGCTGCAAGACCGGGCTGAACACGGTGT
ACCGTGGTATGAGCGCTTTGGTTTGAGTCAGAATCAGAAACTGTTGACGGCAGTGGTGCCCGATTATGCGCAGGTTAATA
ACCGCCTGATACGAGACAAAGCCGCTGCGGTGTTGCATGCGAAACTGAGTGCGCTGGTGAATTTACCGCCGGGCAGCCCT
TTGCGGGCTAAGCGGGCGAAAGCCGGTCATGACCAGTTGAAAGCCTACCTGATGATGGCGCAGCCGGAAAAAGTGGATGC
TGCGTTTTTAACCCGGATACTGGATGAAAATGAACCCGAGCGGGCCGGTATTGCCCCCGCTGTCTGGTTGGATACGGCGT
GGGATTTGTGGAGTTTTTATGCGGAGAATCTGGCCGCGCATCCCGAATGGCGGATAACCCCGGATAAGGCGTTGATTAAA
CAGGTGCGTCAGTTGTTGCTGATCCAGCCAGGTAAGCACAATGCCGAAGCGGCGCTTTACCAGCAGATGCTGCAATCAGT
GGAGAAAAATTATTCGGACCTGACGTTAAGGCAGATGACCGGCGACACCGATGCGGCGCGGCTGTTTACCACCTCTCACG
TGATACCGGGGATGTTTACCCGTCAGGCGTGGGAAGGCGGGATACAAAAGGCGATAGCGGAGGCGGTGGCGTCACGTAAA
GAGGCAATTGACTGGGTGCTGAGTGATAACCGTCAGGCAGTCTCGGCTTCGGTATCGCCCGAAGAGCTGAAAGCCCGTTT
GACCGCCCGTTACTTTACCGATTTTGCCGGGGCGTGGCAGGATTTTCTCAACAGCCTGCGCTGGAACAAAACCCGTAACC
TGTCGGATGTCATCGACCAGCTAACGTTGATGGCGGATGGGCGGCAGTCTCCGCTGATTGCGCTGATGAACACGCTGGCC
TATCAGGGCGAAACCGGGCAACAGGGGCCGAGTTTATCGGACTCATTGGTGAAATCCGCTAAAACGTTATTGAATAAAAA
AACGCCGCGAATGATTAATCAACAGACGGCTGGCTTGCACGGGCCGATGGATGAAACCTTTGGGCCGTTGTTGGCGCTGA
TGGGAGAAAATCAGGCAGAAGGAAGAATCAAGGCGGACAGTACCCTGAATTTACAAACCTTCCTGACGCGGGTAACCGAC
GTGCGTCTGAAACTGCAACAGATGAACAACAGCGACGACCCACCGGCAATGGCGAATGCGCTGGCTAAAACGGTATTTGA
AGGGAAAAGCACTGATTTGACCGATACGCAGGGCTATGGGCGCTTGATAGCCGCCAGTCTGGGCGGCCAATGGCGTGGTT
TTGGTCAGACGCTGTTTGTCGAGCCATTAGCGCAGGCGTGGCAGGGGGTATTGAAACCGGCGACGGCGAATCTGAATAGC
CAGTGGCAGACGGCGATAGTGGACAACTGGGATCGGGCATTTGCGGGTCGTTATCCGTTTGCCGGTGGCGAAAGTGAAAT
CTCATTGCCGATGTTGGGGCAGTTTATCCGGTCAGATTCAGGGCGGATAGAGCAGTTCTTGACGCGTCAGTTAGGCGGGA
TACTGCATAAAGCGGGTAACCAGTGGGTAGTGGATGAGGTGAATGGTCAGGGCGTACAGATTAACCCGGATTTTCTAAAG
GCGATAAATCAGCTCAGTCAGTTATCGGACCTGTTGTTTGCGGATGGCAGTCAGGGATTGCGGTTTGAACTGCGGGCCAG
AGCGGTGCGGGATGTGGTGGAAACTGACCTGACGATAGATGGGCAGAAACTGCGTTATTTTAACCAGATGGAAAGTTGGC
AAAGTTTTCGCTGGCCGGGAGAGACCTATAAACCGGGTGTGATGCTGACGTGGACCAGTGTCAATGCCGGGGCGAGATTG
TTTGGTGATTATCAGGGGAACTGGGGACTCATCCGCTGGCTGGCGCAGGCGAAAGCGGAGAAGCTGGATGAAAGCCGTTA
CCGACTGATATTTACCGCGCCGGATGGATTACCGTTAACCTGGATTTTGCGCACAGAATTGGGCGAAGGGCCGCTGGCGT
TATTAAAACTGCGCGGATTTAAATTGCCGAAAAATATCTTTGTAGTGACGCCGGGTAATCACACGACAATATCAGCGGTA
AATGACGATGATTTGATAGAGGAATAG					