SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000452
Component protein type	TssM
T6SS ID (Type)	T6SS00052 (Type i2)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (1124 a.a.); Nucleotide sequence (3372 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Bernard CS, Brunet YR, Gueguen E, et al. Nooks and crannies in type VI secretion regulation. J Bacteriol. 2010 Aug;192(15):3850-60. doi: 10.1128/JB.00370-10. Epub 2010 May 28. PMID: 20511495

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS16085
Coordinate (Strand)	3819697..3823068 (-)
NCBI ID	WP_011147435.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	3.3e-67	401..717
IcmF_C	PF06744.14	7.6e-21	977..1081

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-8	MKVNRHHQ
Transmembrane helix	9-31	YGYFGYIFITLSLAGIFLMLIYL
Outside	32-45	NQTLLEERGLLKSV
Transmembrane helix	46-68	WYAYATVFPILLIGLFGNAVWWH
Inside	69-352	IRQNKAQKKLRPETERTADRSSQSALVLSDLGDRMRLRYGRFWWYKVRILLVVGEVEQVEAIAPGLTTTHWQEGYRTLLLWGGSLQAEPNTAQLMALRRLRRYRPLNAIVWALTENQSAQPVWMDKALRMLQKQAQQLRWQAPVYLWQVCHSTWSQEGRITQTVGCFFPERCTPDIVATQLQTLVDPLRERGMQQLLEKTAHDFLCRLSANLKQQGIAHWQQVLTPWLAEYGDVVSLRGLMFSLPLKAQTTRLPQIWLPDTAWQGVLEGSRCFHGRRVGMLHTQ
Transmembrane helix	353-375	TVCRGLIVLGLLWGAGMMLSFFT
Outside	376-1123	NRDQLAVVQAAVTDLKVQPENSDAQLMALKDLRNEVDRLQDRAEHGVPWYERFGLSQNQKLLTAVLPDYAQVNNRLIRDKAAAVLHAKLSALVNLPPGSPLRAKRAQAGHDQLKAYLMMARPEKVDAVFLTRILDENEPERAGIAPGVWLDTAWDLWSFYAENLAAHPEWRITADKALIKQVRQLLLLQPGKHNAEAALYQQMLQSVEKNYSDLTLRQMTGDTDAARLFTTSHVIPGMFTRQAWEGGIQKAIAKAVASRKEAIDWVLSDNRQAVSASVSPEELKARLTARYFTDFARVWQDFLNSLRWNKTRNLSDVIDQLTLMADGRQSPLIALMNTLAYQGETGQQGPSLSDSLVKSAKTLLNKKTPRMINQQTAGLHGPMDETFGPLLALMGENQAEGRIKADSTLNLQTFLTRVTDVRLKLQQMNNSDDPPAMANALAKTVFEGKSTDLTDTQGYGRLIAASLGGQWRSFGQTLFVEPLAQAWQGVLKPATANLNSQWQTAIVDNWDRAFAGRYPFAGGDSEISLPMLGQFIRSDSGRIEQFLTRQLGGILHKAGNQWVVDEVNGQGVQINPDFLKAINQLSQLSDLLFADGSQGLRFELRARAVRDVVETDLTIDGQKLRYFNQMESWQSFRWPGETYKPGVMLTWTSVNAGARLFGDYQGNWGLIRWLAQAKAERLDESRYRLIFTAPDGLPLTWILRTELGEGPLALLKLRGFKLPKNIFVVMPGNHTTISAVNDDDLIEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9948	-	-

Protein sequence: 1124 a.a.

						>T6CP000452 NC_005126:c3823068-3819697 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
MKVNRHHQYGYFGYIFITLSLAGIFLMLIYLNQTLLEERGLLKSVWYAYATVFPILLIGLFGNAVWWHIRQNKAQKKLRP
ETERTADRSSQSALVLSDLGDRMRLRYGRFWWYKVRILLVVGEVEQVEAIAPGLTTTHWQEGYRTLLLWGGSLQAEPNTA
QLMALRRLRRYRPLNAIVWALTENQSAQPVWMDKALRMLQKQAQQLRWQAPVYLWQVCHSTWSQEGRITQTVGCFFPERC
TPDIVATQLQTLVDPLRERGMQQLLEKTAHDFLCRLSANLKQQGIAHWQQVLTPWLAEYGDVVSLRGLMFSLPLKAQTTR
LPQIWLPDTAWQGVLEGSRCFHGRRVGMLHTQTVCRGLIVLGLLWGAGMMLSFFTNRDQLAVVQAAVTDLKVQPENSDAQ
LMALKDLRNEVDRLQDRAEHGVPWYERFGLSQNQKLLTAVLPDYAQVNNRLIRDKAAAVLHAKLSALVNLPPGSPLRAKR
AQAGHDQLKAYLMMARPEKVDAVFLTRILDENEPERAGIAPGVWLDTAWDLWSFYAENLAAHPEWRITADKALIKQVRQL
LLLQPGKHNAEAALYQQMLQSVEKNYSDLTLRQMTGDTDAARLFTTSHVIPGMFTRQAWEGGIQKAIAKAVASRKEAIDW
VLSDNRQAVSASVSPEELKARLTARYFTDFARVWQDFLNSLRWNKTRNLSDVIDQLTLMADGRQSPLIALMNTLAYQGET
GQQGPSLSDSLVKSAKTLLNKKTPRMINQQTAGLHGPMDETFGPLLALMGENQAEGRIKADSTLNLQTFLTRVTDVRLKL
QQMNNSDDPPAMANALAKTVFEGKSTDLTDTQGYGRLIAASLGGQWRSFGQTLFVEPLAQAWQGVLKPATANLNSQWQTA
IVDNWDRAFAGRYPFAGGDSEISLPMLGQFIRSDSGRIEQFLTRQLGGILHKAGNQWVVDEVNGQGVQINPDFLKAINQL
SQLSDLLFADGSQGLRFELRARAVRDVVETDLTIDGQKLRYFNQMESWQSFRWPGETYKPGVMLTWTSVNAGARLFGDYQ
GNWGLIRWLAQAKAERLDESRYRLIFTAPDGLPLTWILRTELGEGPLALLKLRGFKLPKNIFVVMPGNHTTISAVNDDDL
IEE*					

Nucleotide sequence: 3372 bp

						>T6CP000452 NC_005126:c3823068-3819697 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
ATGAAAGTTAATCGTCACCACCAATATGGCTATTTTGGGTATATTTTTATTACCCTTAGCCTTGCCGGGATATTTCTCAT
GTTGATCTACTTGAATCAAACATTACTTGAGGAACGGGGGCTGCTTAAATCCGTATGGTATGCCTACGCTACCGTTTTTC
CGATCCTATTAATAGGCTTATTTGGCAATGCTGTCTGGTGGCATATTCGGCAGAATAAAGCGCAGAAAAAACTCAGACCT
GAAACCGAGCGGACAGCGGATCGCTCCAGCCAGTCTGCTTTGGTACTGAGTGACTTGGGCGATCGCATGCGCCTCCGTTA
CGGGCGGTTCTGGTGGTATAAAGTCCGCATTTTACTGGTGGTGGGCGAAGTGGAGCAGGTGGAAGCCATCGCGCCGGGGC
TGACCACGACGCACTGGCAGGAGGGTTATCGCACGCTGTTGCTCTGGGGCGGCAGTTTGCAAGCGGAACCGAATACCGCT
CAATTGATGGCCCTGCGTCGCTTGCGCCGTTACCGCCCGCTCAATGCCATTGTCTGGGCGCTGACCGAAAACCAAAGTGC
GCAGCCGGTATGGATGGACAAGGCCCTGAGGATGTTGCAAAAACAGGCCCAACAGTTACGCTGGCAGGCTCCGGTCTACC
TGTGGCAGGTGTGTCACAGTACCTGGTCTCAGGAAGGTCGTATCACTCAGACCGTCGGCTGTTTTTTTCCTGAACGCTGT
ACCCCGGATATCGTGGCGACACAGTTACAAACGTTGGTTGACCCGTTGCGCGAGCGCGGGATGCAGCAACTGTTGGAGAA
GACGGCCCACGATTTTCTCTGTCGTCTGTCCGCGAACTTGAAACAACAGGGGATTGCGCACTGGCAACAGGTCCTGACGC
CGTGGTTAGCGGAGTATGGTGATGTGGTGTCGCTGCGGGGTTTGATGTTCAGTTTGCCGCTTAAAGCGCAGACGACTCGC
CTCCCGCAGATCTGGCTGCCCGACACGGCATGGCAGGGGGTGTTGGAGGGCAGTCGTTGTTTCCACGGTCGGCGGGTGGG
CATGCTCCACACACAGACCGTTTGTCGTGGCCTGATAGTGCTGGGCTTGTTATGGGGGGCCGGAATGATGCTCTCCTTTT
TCACCAACCGTGACCAGCTAGCCGTGGTGCAGGCGGCGGTAACTGACCTGAAAGTCCAGCCGGAGAATTCTGATGCCCAG
TTGATGGCCTTGAAGGATCTGCGTAACGAGGTGGACCGGCTGCAAGACCGGGCTGAACACGGCGTGCCGTGGTATGAGCG
CTTTGGTTTGAGTCAGAATCAGAAACTGTTGACGGCGGTGCTGCCCGATTATGCGCAGGTTAATAACCGCCTGATACGGG
ACAAAGCCGCTGCGGTGTTGCATGCGAAACTGAGTGCGCTGGTGAATTTACCGCCGGGCAGCCCTTTGCGGGCTAAGCGG
GCGCAAGCCGGTCATGACCAGTTGAAAGCCTACCTGATGATGGCGCGGCCGGAAAAAGTGGATGCTGTGTTTTTAACCCG
GATACTGGATGAAAATGAACCCGAGCGGGCCGGTATTGCCCCCGGTGTCTGGCTGGATACGGCGTGGGATTTGTGGAGTT
TTTATGCGGAGAATCTGGCCGCGCATCCCGAATGGCGGATAACCGCGGATAAGGCGTTGATTAAACAGGTGCGTCAGTTG
TTGCTGCTCCAGCCAGGTAAGCACAATGCCGAAGCGGCGCTTTACCAGCAGATGCTGCAATCAGTGGAGAAAAATTATTC
GGACCTGACGTTAAGGCAGATGACCGGCGACACCGATGCGGCGCGGCTGTTTACCACCTCTCACGTGATACCGGGGATGT
TTACCCGTCAGGCGTGGGAAGGCGGGATACAAAAGGCGATAGCGAAGGCGGTGGCGTCACGTAAAGAGGCAATTGACTGG
GTGCTGAGTGATAACCGTCAGGCAGTCTCGGCGTCGGTATCGCCCGAAGAGCTGAAAGCCCGTTTGACCGCCCGTTACTT
TACCGATTTCGCCAGGGTGTGGCAGGATTTTCTCAACAGCCTGCGCTGGAACAAAACCCGTAACCTGTCGGATGTCATCG
ACCAGCTAACGTTGATGGCGGATGGACGGCAGTCTCCGCTGATTGCGCTGATGAACACGCTGGCCTATCAGGGCGAAACC
GGGCAACAGGGGCCGAGTTTATCGGACTCATTGGTGAAATCCGCTAAAACGTTATTGAATAAAAAAACGCCGCGAATGAT
TAATCAACAGACGGCTGGCTTGCACGGGCCGATGGATGAAACCTTTGGGCCGTTGCTGGCGCTGATGGGAGAAAATCAGG
CAGAAGGAAGAATCAAGGCGGACAGTACCCTGAATTTACAAACCTTCTTGACCCGGGTAACCGACGTGCGTCTGAAACTG
CAACAGATGAACAACAGCGACGACCCACCGGCAATGGCGAATGCGCTGGCTAAGACGGTATTTGAAGGGAAAAGTACTGA
TTTGACCGATACTCAGGGTTATGGGCGCTTGATAGCCGCCAGTCTGGGCGGCCAATGGCGGAGTTTTGGTCAGACGCTGT
TTGTCGAGCCATTAGCGCAGGCGTGGCAGGGAGTATTGAAACCGGCGACGGCGAATCTGAATAGCCAGTGGCAGACGGCG
ATAGTGGACAACTGGGATCGGGCATTTGCGGGTCGTTATCCGTTTGCCGGTGGCGACAGTGAAATTTCATTGCCGATGTT
GGGGCAATTTATCCGGTCAGATTCGGGGCGGATAGAGCAGTTCTTGACGCGTCAGTTAGGCGGGATACTGCATAAAGCGG
GTAACCAGTGGGTAGTGGATGAGGTGAATGGTCAGGGCGTGCAGATTAACCCGGATTTTCTAAAGGCGATAAATCAGCTC
AGTCAGTTATCGGACCTGTTGTTTGCGGATGGCAGTCAGGGATTGCGGTTTGAACTGCGGGCCAGAGCGGTGCGGGATGT
GGTGGAAACTGACCTGACGATAGATGGGCAGAAACTGCGTTATTTTAACCAGATGGAAAGCTGGCAGAGCTTTCGCTGGC
CGGGAGAGACCTATAAACCGGGTGTGATGCTGACGTGGACCAGTGTCAATGCCGGGGCGAGATTGTTTGGTGATTATCAG
GGGAACTGGGGGCTGATCCGCTGGCTGGCACAGGCGAAAGCGGAGAGGCTGGATGAAAGCCGTTACCGACTGATATTTAC
CGCGCCGGATGGATTACCGTTAACCTGGATTTTGCGCACAGAATTGGGCGAAGGGCCGCTGGCGTTATTAAAACTGCGCG
GATTTAAATTGCCGAAAAATATCTTTGTAGTGATGCCGGGTAATCACACGACAATATCAGCGGTAAATGACGATGATTTG
ATAGAGGAATAG					