SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000449
Component protein type	TssJ
T6SS ID (Type)	T6SS00052 (Type i2)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (180 a.a.); Nucleotide sequence (540 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Bernard CS, Brunet YR, Gueguen E, et al. Nooks and crannies in type VI secretion regulation. J Bacteriol. 2010 Aug;192(15):3850-60. doi: 10.1128/JB.00370-10. Epub 2010 May 28. PMID: 20511495

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS16045
Coordinate (Strand)	3810485..3811024 (-)
NCBI ID	WP_011147427.1
RefSeq	NC_005126
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	5.2e-26	46..168

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-179	MLTIPFYKMVPVMATIALLLTGCGLTQTVTDGTAAATRSFFHKRVKTLHLDFIPRAAVNTDTGESVALSVPALVRVYQLRDNNAIKKVDYADLLDDGDRILGADCLASEEVVVKPGSHASLDMPMAAQAKYVAVVGLFRNPDVAKGTWRRIIKRNELLPDSPRIIALNAGGLTLLPEKG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9980	pos: 22-23. LTG-CG.	MLTIPFYKMVPVMATIALLLTG

Protein sequence: 180 a.a.

						>T6CP000449 NC_005126:c3811024-3810485 [Photorhabdus laumondii subsp. laumondii TTO1] [TssJ]
MLTIPFYKMVPVMATIALLLTGCGLTQTVTDGTAAATRSFFHKRVKTLHLDFIPRAAVNTDTGESVALSVPALVRVYQLR
DNNAIKKVDYADLLDDGDRILGADCLASEEVVVKPGSHASLDMPMAAQAKYVAVVGLFRNPDVAKGTWRRIIKRNELLPD
SPRIIALNAGGLTLLPEKG*					

Nucleotide sequence: 540 bp

						>T6CP000449 NC_005126:c3811024-3810485 [Photorhabdus laumondii subsp. laumondii TTO1] [TssJ]
ATGTTAACTATCCCTTTTTATAAAATGGTGCCGGTAATGGCGACGATAGCATTACTACTGACAGGCTGTGGTCTGACTCA
GACCGTGACTGATGGCACGGCAGCGGCCACCCGGTCCTTTTTTCATAAGCGGGTGAAAACGTTGCATCTGGATTTTATTC
CACGGGCAGCGGTTAATACCGACACGGGAGAAAGTGTTGCTCTGTCCGTTCCGGCATTAGTTCGGGTTTATCAGTTGCGG
GATAACAACGCTATAAAAAAGGTGGATTATGCGGATTTGTTGGATGACGGCGACCGGATACTGGGCGCTGATTGTCTGGC
GAGTGAAGAGGTAGTGGTGAAACCGGGTAGTCACGCATCGCTTGATATGCCGATGGCGGCGCAGGCGAAATATGTGGCGG
TGGTGGGATTATTCAGAAACCCCGATGTAGCCAAAGGGACTTGGCGTCGGATCATAAAACGCAACGAGTTGTTACCAGAT
AGTCCTCGTATTATTGCGCTTAATGCCGGTGGCTTGACTTTATTACCTGAAAAGGGGTGA					