Detailed information of component protein
Summary
Component ID | T6CP000437 |
Component protein type | TssM |
T6SS ID (Type) | T6SS00051 (Type i4b) |
Strain | Photorhabdus luminescens subsp. laumondii TTO1 |
Replicon | chromosome |
Sequence | Protein sequence (1170 a.a.); Nucleotide sequence (3510 bp) |
Reference |
External database links
Locus tag (Gene) | PLU_RS11420 |
Coordinate (Strand) | 2687306..2690815 (-) |
NCBI ID | WP_011146536.1 |
RefSeq | NC_005126 |
Uniprot ID | - |
KEGG ID | - |
PDB ID | - |
Pfam domain hit(s)
Domain | Pfam ID | E-value | Aligned region |
---|---|---|---|
IcmF-related_N | PF14331.8 | 3.3e-31 | 173..362 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
Prediction | Region | Sequence |
---|---|---|
Outside | 1-3 | MKK |
Transmembrane helix | 4-26 | LFYAIGWLSVMVILLLLSLTIAV |
Inside | 27-32 | AFSWPT |
Transmembrane helix | 33-55 | IGGLLLFICLLLLLLLLRVAMAF |
Outside | 56-1169 | MPEIMNKLRWLNKFWRKGNNRLEYLLYQHWRQGGYLQSWRRFRSLLHRNEPVPPWFLVVGERGNGKQKLLTRCNVVPMTGKNLPALTENTFTCRWWFFRRAMYMVVSGRYTEGQSLYRQAWLRLISWIAQVRKPAGVLVCLSVEQLLNSDNRALYIAARQVRAQLEPLQIRLERRLPIWLILTHSETLPGFDHWCTQLSVEQHAELLGDFIEHHTVGNVTDVLDKSMNTIVNTLKMMRLKLLNQQRQQPNAEILALPETVAQLQPALGCYLNALFDQDHYLEHGLLRGLFFTAEHQTAGVFSQRLLGEYLPDQVQHSQDQRSHNVLLTGWRTLTKRVCVSLLSLMLVYGVGNALMTTWRGVTQLENIHEADPLERSYLRYQKVEYWLQETLPSLFFYPVTHTMEQRMAQQYQQQAPVGAFNPESVAAHLLARFHNAETPQKRLLVLHWARFINIEQAMTHGATLETLQRMPAYSASLLSGENDPLSAEQRVALRLAQYRQGNAQQAFDQWRNVLQTLLEDSNNWQWLLADEQPTGTHSLTLADFWPLASDDVATINTRIRGIYTQAGEQDIQATLGELAQALNRPAFFTPLQRSFYQQYQNQRQSEWLAFAQAMPQGEMLVRGKKNWQALILAAGQYDSPYITFLTQLERDLSSIEQADRQPWLQTQQRLWQLHGYVQKSGMMQRVSLGNFSVRQRIMSWLGISPQISVRLDDTALTRYRAYRQAVQLNSQRELLSDMETEVLVKNALNNEKGELDDSGLRALFNRFTLWHHETESKQSAGVNETLWRLYQGDAHLVLRYAFLSAASRLQTQWESKVIWPMEKLSRQELFDGRELNARLYEYSNAFVRDTAGYALDIRPEGIKRQEIEDVVFPFSDDFIFYINNLIQPNDLLSATSDIRRRLQEKLALLESEQQLTTAQQEPEQEQKPSAEVTIVSQPATANRGAKVLPVGSSVTLSCDDGVQQLKSMNFNDSMTLHWSPGTCGKVQIDIYFPRFTLSKSYIGSDGLLQFMHAFSRGELTLAAQAFPQRRDELSALGINNITVRYQVSESTAVSTLYQQWQQHQQQQTALLEQRNRLDSQLLNLSEPTVAKGTLSTLPSQITTHWNELRKPKSE |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction | Probability | Cleavage site | Signal peptide sequence |
---|---|---|---|
Other | 0.9926 | - | - |
Protein sequence: 1170 a.a.
>T6CP000437 NC_005126:c2690815-2687306 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
MKKLFYAIGWLSVMVILLLLSLTIAVAFSWPTIGGLLLFICLLLLLLLLRVAMAFMPEIMNKLRWLNKFWRKGNNRLEYL
LYQHWRQGGYLQSWRRFRSLLHRNEPVPPWFLVVGERGNGKQKLLTRCNVVPMTGKNLPALTENTFTCRWWFFRRAMYMV
VSGRYTEGQSLYRQAWLRLISWIAQVRKPAGVLVCLSVEQLLNSDNRALYIAARQVRAQLEPLQIRLERRLPIWLILTHS
ETLPGFDHWCTQLSVEQHAELLGDFIEHHTVGNVTDVLDKSMNTIVNTLKMMRLKLLNQQRQQPNAEILALPETVAQLQP
ALGCYLNALFDQDHYLEHGLLRGLFFTAEHQTAGVFSQRLLGEYLPDQVQHSQDQRSHNVLLTGWRTLTKRVCVSLLSLM
LVYGVGNALMTTWRGVTQLENIHEADPLERSYLRYQKVEYWLQETLPSLFFYPVTHTMEQRMAQQYQQQAPVGAFNPESV
AAHLLARFHNAETPQKRLLVLHWARFINIEQAMTHGATLETLQRMPAYSASLLSGENDPLSAEQRVALRLAQYRQGNAQQ
AFDQWRNVLQTLLEDSNNWQWLLADEQPTGTHSLTLADFWPLASDDVATINTRIRGIYTQAGEQDIQATLGELAQALNRP
AFFTPLQRSFYQQYQNQRQSEWLAFAQAMPQGEMLVRGKKNWQALILAAGQYDSPYITFLTQLERDLSSIEQADRQPWLQ
TQQRLWQLHGYVQKSGMMQRVSLGNFSVRQRIMSWLGISPQISVRLDDTALTRYRAYRQAVQLNSQRELLSDMETEVLVK
NALNNEKGELDDSGLRALFNRFTLWHHETESKQSAGVNETLWRLYQGDAHLVLRYAFLSAASRLQTQWESKVIWPMEKLS
RQELFDGRELNARLYEYSNAFVRDTAGYALDIRPEGIKRQEIEDVVFPFSDDFIFYINNLIQPNDLLSATSDIRRRLQEK
LALLESEQQLTTAQQEPEQEQKPSAEVTIVSQPATANRGAKVLPVGSSVTLSCDDGVQQLKSMNFNDSMTLHWSPGTCGK
VQIDIYFPRFTLSKSYIGSDGLLQFMHAFSRGELTLAAQAFPQRRDELSALGINNITVRYQVSESTAVSTLYQQWQQHQQ
QQTALLEQRNRLDSQLLNLSEPTVAKGTLSTLPSQITTHWNELRKPKSE*
MKKLFYAIGWLSVMVILLLLSLTIAVAFSWPTIGGLLLFICLLLLLLLLRVAMAFMPEIMNKLRWLNKFWRKGNNRLEYL
LYQHWRQGGYLQSWRRFRSLLHRNEPVPPWFLVVGERGNGKQKLLTRCNVVPMTGKNLPALTENTFTCRWWFFRRAMYMV
VSGRYTEGQSLYRQAWLRLISWIAQVRKPAGVLVCLSVEQLLNSDNRALYIAARQVRAQLEPLQIRLERRLPIWLILTHS
ETLPGFDHWCTQLSVEQHAELLGDFIEHHTVGNVTDVLDKSMNTIVNTLKMMRLKLLNQQRQQPNAEILALPETVAQLQP
ALGCYLNALFDQDHYLEHGLLRGLFFTAEHQTAGVFSQRLLGEYLPDQVQHSQDQRSHNVLLTGWRTLTKRVCVSLLSLM
LVYGVGNALMTTWRGVTQLENIHEADPLERSYLRYQKVEYWLQETLPSLFFYPVTHTMEQRMAQQYQQQAPVGAFNPESV
AAHLLARFHNAETPQKRLLVLHWARFINIEQAMTHGATLETLQRMPAYSASLLSGENDPLSAEQRVALRLAQYRQGNAQQ
AFDQWRNVLQTLLEDSNNWQWLLADEQPTGTHSLTLADFWPLASDDVATINTRIRGIYTQAGEQDIQATLGELAQALNRP
AFFTPLQRSFYQQYQNQRQSEWLAFAQAMPQGEMLVRGKKNWQALILAAGQYDSPYITFLTQLERDLSSIEQADRQPWLQ
TQQRLWQLHGYVQKSGMMQRVSLGNFSVRQRIMSWLGISPQISVRLDDTALTRYRAYRQAVQLNSQRELLSDMETEVLVK
NALNNEKGELDDSGLRALFNRFTLWHHETESKQSAGVNETLWRLYQGDAHLVLRYAFLSAASRLQTQWESKVIWPMEKLS
RQELFDGRELNARLYEYSNAFVRDTAGYALDIRPEGIKRQEIEDVVFPFSDDFIFYINNLIQPNDLLSATSDIRRRLQEK
LALLESEQQLTTAQQEPEQEQKPSAEVTIVSQPATANRGAKVLPVGSSVTLSCDDGVQQLKSMNFNDSMTLHWSPGTCGK
VQIDIYFPRFTLSKSYIGSDGLLQFMHAFSRGELTLAAQAFPQRRDELSALGINNITVRYQVSESTAVSTLYQQWQQHQQ
QQTALLEQRNRLDSQLLNLSEPTVAKGTLSTLPSQITTHWNELRKPKSE*
Nucleotide sequence: 3510 bp
>T6CP000437 NC_005126:c2690815-2687306 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
ATGAAAAAATTATTTTATGCCATTGGCTGGCTTAGCGTGATGGTTATCCTTTTATTACTCAGTTTAACTATTGCGGTGGC
ATTTTCATGGCCGACAATAGGTGGTTTATTACTATTTATTTGCCTATTACTGTTGTTATTATTGCTGCGTGTGGCAATGG
CATTCATGCCAGAAATAATGAATAAACTGCGCTGGTTAAATAAATTCTGGCGGAAAGGAAATAACCGTCTGGAATATTTA
CTCTATCAGCACTGGAGACAAGGAGGATATTTACAAAGTTGGCGTCGATTCCGTTCACTGTTACACCGTAACGAACCCGT
TCCACCGTGGTTTCTGGTGGTAGGGGAACGGGGCAATGGTAAACAGAAGTTGTTAACACGCTGCAATGTGGTGCCCATGA
CGGGGAAAAATCTGCCAGCGCTGACAGAAAACACCTTTACCTGTCGCTGGTGGTTCTTCCGCCGGGCAATGTACATGGTT
GTTTCCGGCCGTTATACCGAAGGTCAATCCCTGTACCGGCAAGCCTGGTTGCGTCTAATCAGTTGGATCGCACAAGTACG
TAAACCCGCTGGCGTACTCGTTTGCTTATCGGTGGAACAGTTGCTAAACAGCGACAATAGAGCGCTCTATATCGCCGCAC
GTCAGGTACGTGCTCAGTTGGAACCATTACAGATACGTCTGGAACGCCGCTTACCCATCTGGCTGATCCTTACCCACAGC
GAAACATTGCCGGGGTTTGACCATTGGTGCACCCAACTGTCAGTAGAACAACATGCTGAATTGCTAGGCGATTTTATCGA
GCATCATACGGTGGGTAATGTCACCGACGTGCTGGATAAGAGCATGAACACCATTGTTAACACGTTGAAAATGATGCGCC
TTAAGTTGCTCAATCAGCAACGGCAGCAACCCAATGCAGAAATCTTAGCGTTACCAGAAACGGTAGCTCAACTGCAACCT
GCACTGGGTTGTTACTTGAATGCACTGTTCGACCAAGACCATTATCTGGAGCATGGTCTGTTGCGTGGCCTGTTTTTTAC
CGCCGAGCATCAAACTGCTGGCGTTTTCAGCCAACGTTTGTTGGGAGAATACCTGCCAGATCAAGTTCAACATAGCCAAG
ATCAACGTAGCCATAACGTATTGCTGACTGGCTGGCGGACATTGACCAAACGCGTATGCGTTAGCCTACTCAGTTTGATG
CTGGTGTACGGTGTCGGCAATGCATTGATGACGACCTGGCGTGGCGTTACGCAACTTGAAAATATCCATGAGGCAGACCC
GCTTGAGCGGAGTTACTTGCGCTACCAAAAAGTTGAATACTGGTTGCAGGAAACCTTGCCATCTCTGTTTTTCTATCCGG
TCACCCATACGATGGAACAACGGATGGCACAGCAATACCAGCAACAAGCACCTGTTGGTGCATTCAACCCGGAGTCGGTA
GCGGCACACTTACTAGCACGTTTCCATAACGCGGAAACACCGCAAAAACGACTGTTGGTTTTACATTGGGCACGCTTTAT
TAACATAGAACAGGCAATGACACATGGCGCTACGCTAGAAACTTTACAGCGTATGCCTGCTTATTCAGCTTCACTATTAA
GTGGTGAAAACGATCCTTTATCCGCGGAACAACGCGTCGCACTTCGTCTGGCGCAATATCGCCAAGGCAATGCACAGCAA
GCATTTGATCAGTGGCGAAATGTATTGCAAACACTACTAGAAGATAGCAATAACTGGCAGTGGCTATTAGCCGACGAACA
ACCGACTGGCACGCATTCGCTGACACTGGCTGATTTCTGGCCGCTCGCCAGCGATGATGTTGCCACAATTAACACACGTA
TTCGTGGTATCTATACCCAAGCAGGTGAGCAAGATATTCAGGCAACCTTAGGAGAACTGGCACAAGCCCTTAACAGACCG
GCATTCTTTACTCCTTTACAGCGCTCGTTTTATCAACAGTATCAAAATCAGCGCCAATCAGAGTGGTTAGCATTCGCTCA
GGCTATGCCTCAAGGAGAGATGTTAGTTCGAGGCAAGAAAAACTGGCAAGCGCTGATATTAGCAGCGGGCCAATATGACA
GCCCCTATATCACCTTTCTGACCCAGCTAGAACGTGATTTATCCTCGATAGAGCAGGCGGATCGGCAGCCTTGGTTACAG
ACACAGCAGCGTTTATGGCAACTGCATGGTTATGTGCAAAAAAGTGGCATGATGCAACGAGTCTCGCTAGGGAATTTCTC
TGTGCGCCAGCGCATCATGTCATGGCTTGGAATTTCACCGCAGATCTCGGTACGGCTGGATGATACCGCGCTGACACGCT
ATCGCGCCTACCGACAGGCGGTGCAGTTAAATAGCCAACGCGAGTTGCTCAGTGATATGGAAACCGAGGTGTTGGTTAAA
AATGCGCTAAATAACGAGAAAGGAGAGTTGGATGATAGCGGTTTACGCGCATTGTTTAACCGTTTCACCCTGTGGCACCA
TGAGACTGAAAGCAAACAGAGTGCAGGAGTAAATGAAACCTTATGGCGGCTCTATCAGGGTGATGCGCATCTGGTACTGC
GTTACGCCTTCTTAAGTGCTGCCAGCCGCTTGCAAACTCAGTGGGAAAGCAAAGTGATTTGGCCGATGGAAAAATTGAGC
AGACAGGAACTGTTTGATGGCCGGGAATTGAACGCGCGTTTGTATGAATACAGCAATGCATTTGTACGGGATACCGCAGG
GTATGCGCTGGATATCCGTCCTGAAGGGATTAAACGTCAGGAAATAGAGGATGTAGTCTTTCCGTTTAGCGACGATTTCA
TTTTCTATATCAATAACCTCATTCAACCGAATGACCTACTTTCAGCGACATCGGATATTCGCCGTCGTCTGCAAGAGAAA
CTCGCCTTATTGGAAAGCGAACAGCAACTGACGACAGCACAGCAGGAACCGGAGCAAGAACAGAAGCCATCGGCGGAAGT
AACCATTGTCAGTCAGCCGGCGACCGCTAACCGGGGGGCAAAAGTATTGCCAGTAGGCAGTTCAGTGACGTTGAGTTGTG
ATGATGGCGTACAGCAGCTTAAGAGTATGAATTTCAATGACAGCATGACGCTGCACTGGAGCCCAGGAACATGCGGTAAA
GTACAAATTGATATCTATTTTCCTCGCTTTACCCTAAGCAAAAGTTACATTGGCTCCGATGGTTTGCTGCAATTTATGCA
CGCTTTCTCCCGCGGTGAACTGACATTAGCAGCTCAAGCGTTCCCGCAACGGCGGGATGAATTAAGTGCGCTGGGTATCA
ATAACATCACTGTGCGCTATCAAGTCAGCGAAAGCACAGCGGTTTCAACACTCTACCAACAATGGCAGCAACATCAGCAA
CAACAAACGGCGTTATTGGAACAACGCAACCGACTGGATAGCCAGTTGTTAAATTTAAGTGAACCTACAGTGGCAAAGGG
AACTCTCTCTACTTTGCCATCACAGATCACCACCCATTGGAACGAATTAAGGAAACCTAAAAGTGAGTAA
ATGAAAAAATTATTTTATGCCATTGGCTGGCTTAGCGTGATGGTTATCCTTTTATTACTCAGTTTAACTATTGCGGTGGC
ATTTTCATGGCCGACAATAGGTGGTTTATTACTATTTATTTGCCTATTACTGTTGTTATTATTGCTGCGTGTGGCAATGG
CATTCATGCCAGAAATAATGAATAAACTGCGCTGGTTAAATAAATTCTGGCGGAAAGGAAATAACCGTCTGGAATATTTA
CTCTATCAGCACTGGAGACAAGGAGGATATTTACAAAGTTGGCGTCGATTCCGTTCACTGTTACACCGTAACGAACCCGT
TCCACCGTGGTTTCTGGTGGTAGGGGAACGGGGCAATGGTAAACAGAAGTTGTTAACACGCTGCAATGTGGTGCCCATGA
CGGGGAAAAATCTGCCAGCGCTGACAGAAAACACCTTTACCTGTCGCTGGTGGTTCTTCCGCCGGGCAATGTACATGGTT
GTTTCCGGCCGTTATACCGAAGGTCAATCCCTGTACCGGCAAGCCTGGTTGCGTCTAATCAGTTGGATCGCACAAGTACG
TAAACCCGCTGGCGTACTCGTTTGCTTATCGGTGGAACAGTTGCTAAACAGCGACAATAGAGCGCTCTATATCGCCGCAC
GTCAGGTACGTGCTCAGTTGGAACCATTACAGATACGTCTGGAACGCCGCTTACCCATCTGGCTGATCCTTACCCACAGC
GAAACATTGCCGGGGTTTGACCATTGGTGCACCCAACTGTCAGTAGAACAACATGCTGAATTGCTAGGCGATTTTATCGA
GCATCATACGGTGGGTAATGTCACCGACGTGCTGGATAAGAGCATGAACACCATTGTTAACACGTTGAAAATGATGCGCC
TTAAGTTGCTCAATCAGCAACGGCAGCAACCCAATGCAGAAATCTTAGCGTTACCAGAAACGGTAGCTCAACTGCAACCT
GCACTGGGTTGTTACTTGAATGCACTGTTCGACCAAGACCATTATCTGGAGCATGGTCTGTTGCGTGGCCTGTTTTTTAC
CGCCGAGCATCAAACTGCTGGCGTTTTCAGCCAACGTTTGTTGGGAGAATACCTGCCAGATCAAGTTCAACATAGCCAAG
ATCAACGTAGCCATAACGTATTGCTGACTGGCTGGCGGACATTGACCAAACGCGTATGCGTTAGCCTACTCAGTTTGATG
CTGGTGTACGGTGTCGGCAATGCATTGATGACGACCTGGCGTGGCGTTACGCAACTTGAAAATATCCATGAGGCAGACCC
GCTTGAGCGGAGTTACTTGCGCTACCAAAAAGTTGAATACTGGTTGCAGGAAACCTTGCCATCTCTGTTTTTCTATCCGG
TCACCCATACGATGGAACAACGGATGGCACAGCAATACCAGCAACAAGCACCTGTTGGTGCATTCAACCCGGAGTCGGTA
GCGGCACACTTACTAGCACGTTTCCATAACGCGGAAACACCGCAAAAACGACTGTTGGTTTTACATTGGGCACGCTTTAT
TAACATAGAACAGGCAATGACACATGGCGCTACGCTAGAAACTTTACAGCGTATGCCTGCTTATTCAGCTTCACTATTAA
GTGGTGAAAACGATCCTTTATCCGCGGAACAACGCGTCGCACTTCGTCTGGCGCAATATCGCCAAGGCAATGCACAGCAA
GCATTTGATCAGTGGCGAAATGTATTGCAAACACTACTAGAAGATAGCAATAACTGGCAGTGGCTATTAGCCGACGAACA
ACCGACTGGCACGCATTCGCTGACACTGGCTGATTTCTGGCCGCTCGCCAGCGATGATGTTGCCACAATTAACACACGTA
TTCGTGGTATCTATACCCAAGCAGGTGAGCAAGATATTCAGGCAACCTTAGGAGAACTGGCACAAGCCCTTAACAGACCG
GCATTCTTTACTCCTTTACAGCGCTCGTTTTATCAACAGTATCAAAATCAGCGCCAATCAGAGTGGTTAGCATTCGCTCA
GGCTATGCCTCAAGGAGAGATGTTAGTTCGAGGCAAGAAAAACTGGCAAGCGCTGATATTAGCAGCGGGCCAATATGACA
GCCCCTATATCACCTTTCTGACCCAGCTAGAACGTGATTTATCCTCGATAGAGCAGGCGGATCGGCAGCCTTGGTTACAG
ACACAGCAGCGTTTATGGCAACTGCATGGTTATGTGCAAAAAAGTGGCATGATGCAACGAGTCTCGCTAGGGAATTTCTC
TGTGCGCCAGCGCATCATGTCATGGCTTGGAATTTCACCGCAGATCTCGGTACGGCTGGATGATACCGCGCTGACACGCT
ATCGCGCCTACCGACAGGCGGTGCAGTTAAATAGCCAACGCGAGTTGCTCAGTGATATGGAAACCGAGGTGTTGGTTAAA
AATGCGCTAAATAACGAGAAAGGAGAGTTGGATGATAGCGGTTTACGCGCATTGTTTAACCGTTTCACCCTGTGGCACCA
TGAGACTGAAAGCAAACAGAGTGCAGGAGTAAATGAAACCTTATGGCGGCTCTATCAGGGTGATGCGCATCTGGTACTGC
GTTACGCCTTCTTAAGTGCTGCCAGCCGCTTGCAAACTCAGTGGGAAAGCAAAGTGATTTGGCCGATGGAAAAATTGAGC
AGACAGGAACTGTTTGATGGCCGGGAATTGAACGCGCGTTTGTATGAATACAGCAATGCATTTGTACGGGATACCGCAGG
GTATGCGCTGGATATCCGTCCTGAAGGGATTAAACGTCAGGAAATAGAGGATGTAGTCTTTCCGTTTAGCGACGATTTCA
TTTTCTATATCAATAACCTCATTCAACCGAATGACCTACTTTCAGCGACATCGGATATTCGCCGTCGTCTGCAAGAGAAA
CTCGCCTTATTGGAAAGCGAACAGCAACTGACGACAGCACAGCAGGAACCGGAGCAAGAACAGAAGCCATCGGCGGAAGT
AACCATTGTCAGTCAGCCGGCGACCGCTAACCGGGGGGCAAAAGTATTGCCAGTAGGCAGTTCAGTGACGTTGAGTTGTG
ATGATGGCGTACAGCAGCTTAAGAGTATGAATTTCAATGACAGCATGACGCTGCACTGGAGCCCAGGAACATGCGGTAAA
GTACAAATTGATATCTATTTTCCTCGCTTTACCCTAAGCAAAAGTTACATTGGCTCCGATGGTTTGCTGCAATTTATGCA
CGCTTTCTCCCGCGGTGAACTGACATTAGCAGCTCAAGCGTTCCCGCAACGGCGGGATGAATTAAGTGCGCTGGGTATCA
ATAACATCACTGTGCGCTATCAAGTCAGCGAAAGCACAGCGGTTTCAACACTCTACCAACAATGGCAGCAACATCAGCAA
CAACAAACGGCGTTATTGGAACAACGCAACCGACTGGATAGCCAGTTGTTAAATTTAAGTGAACCTACAGTGGCAAAGGG
AACTCTCTCTACTTTGCCATCACAGATCACCACCCATTGGAACGAATTAAGGAAACCTAAAAGTGAGTAA