SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000435
Component protein type	TssB
T6SS ID (Type)	T6SS00050 (Type i1)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (166 a.a.); Nucleotide sequence (498 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS01810
Coordinate (Strand)	400306..400803 (-)
NCBI ID	WP_011144761.1
RefSeq	NC_005126
Uniprot ID	A0A6L9JPC3_PHOLM, Q7N9G4_PHOLL
KEGG ID	plum:A4R40_01815, plu:plu0372
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	1.7e-54	8..159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-165	MSKNSSGSVAPKERINIKYVPNTGDQVAEIELPLNLLIVGDLKGTKEDTPIEERQAVSVHKNNFNAVMNEANINLTFSVPNRLNDNNGDDLPINLEIKSLNDFSPDNVAKKVPELNKLLELREALVALKGPLGNIPAFRTRLQELLGDEKVREQLLKELEIVNQK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9861	-	-

Protein sequence: 166 a.a.

						>T6CP000435 NC_005126:c400803-400306 [Photorhabdus laumondii subsp. laumondii TTO1] [TssB]
MSKNSSGSVAPKERINIKYVPNTGDQVAEIELPLNLLIVGDLKGTKEDTPIEERQAVSVHKNNFNAVMNEANINLTFSVP
NRLNDNNGDDLPINLEIKSLNDFSPDNVAKKVPELNKLLELREALVALKGPLGNIPAFRTRLQELLGDEKVREQLLKELE
IVNQK*					

Nucleotide sequence: 498 bp

						>T6CP000435 NC_005126:c400803-400306 [Photorhabdus laumondii subsp. laumondii TTO1] [TssB]
ATGAGTAAAAATAGTTCAGGTAGCGTAGCGCCAAAAGAAAGGATTAATATCAAATACGTTCCGAATACCGGTGATCAAGT
AGCCGAGATTGAATTACCCCTTAACCTACTTATCGTTGGTGACCTCAAAGGAACAAAAGAAGATACGCCAATTGAAGAAC
GGCAAGCTGTTTCTGTGCACAAGAATAACTTTAATGCCGTGATGAATGAAGCCAATATCAATCTCACCTTTAGTGTCCCC
AATCGCCTCAACGATAATAACGGAGATGACCTGCCAATTAACCTGGAAATAAAATCACTCAATGATTTCTCACCCGATAA
CGTAGCGAAGAAAGTGCCTGAATTAAATAAACTTTTAGAGCTGCGTGAGGCACTTGTCGCACTAAAAGGGCCATTAGGAA
ATATTCCGGCATTCCGCACCCGCTTACAAGAGTTATTGGGTGATGAGAAAGTCCGCGAACAACTTCTAAAAGAGCTTGAA
ATCGTCAATCAAAAGTAA					