SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000432
Component protein type	TssG
T6SS ID (Type)	T6SS00050 (Type i1)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (351 a.a.); Nucleotide sequence (1053 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS01790
Coordinate (Strand)	395492..396544 (-)
NCBI ID	WP_041379883.1
RefSeq	NC_005126
Uniprot ID	A0A6L9JP69_PHOLM
KEGG ID	plum:A4R40_01795
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	1.7e-88	25..336

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-350	MAGTDRSTCVDVTSENSPQNQILDVSRYNFHQLVELFNQLAVAWEKTGQTNRPDREAIQFKSSASLAFPTRDIVSAKRSKEGRFQVEVSFLGLHGSQSPMPGYYLDSLAWEDAQDENRLTNFLNIFNHSSIVMLHQIWRKYRYYICFDKNGQDDFSQRMFALVGLGSNVNRSHLNINHSKMLAYAGLLASPGRSPEVICSLVSHCFDLPDVTVHGWQIRKIAIEPSQQNRLGGMLKIRGQKQKVKQSVIGKNFFLGSWVRSCNSKFILCINNLSREHFLSFLPDGKNFLPLVMFLCFVLRDQMAWDLRLGLAENQAGGMVLGTKLNNNLGWTSFLGEPEKKPFITISVNG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9919	-	-

Protein sequence: 351 a.a.

						>T6CP000432 NC_005126:c396544-395492 [Photorhabdus laumondii subsp. laumondii TTO1] [TssG]
MAGTDRSTCVDVTSENSPQNQILDVSRYNFHQLVELFNQLAVAWEKTGQTNRPDREAIQFKSSASLAFPTRDIVSAKRSK
EGRFQVEVSFLGLHGSQSPMPGYYLDSLAWEDAQDENRLTNFLNIFNHSSIVMLHQIWRKYRYYICFDKNGQDDFSQRMF
ALVGLGSNVNRSHLNINHSKMLAYAGLLASPGRSPEVICSLVSHCFDLPDVTVHGWQIRKIAIEPSQQNRLGGMLKIRGQ
KQKVKQSVIGKNFFLGSWVRSCNSKFILCINNLSREHFLSFLPDGKNFLPLVMFLCFVLRDQMAWDLRLGLAENQAGGMV
LGTKLNNNLGWTSFLGEPEKKPFITISVNG*					

Nucleotide sequence: 1053 bp

						>T6CP000432 NC_005126:c396544-395492 [Photorhabdus laumondii subsp. laumondii TTO1] [TssG]
ATGGCCGGAACAGACAGGTCAACATGCGTTGATGTAACGAGTGAAAACTCGCCACAAAATCAGATATTAGATGTGTCAAG
GTATAACTTTCATCAATTGGTCGAATTGTTCAACCAATTGGCGGTAGCGTGGGAAAAAACTGGGCAAACCAACCGTCCGG
ACCGTGAAGCAATTCAGTTTAAATCCAGCGCCAGCCTGGCTTTCCCAACCAGAGATATTGTCTCTGCGAAACGTTCTAAA
GAGGGACGTTTCCAGGTGGAAGTCTCTTTTCTGGGGCTACATGGCAGCCAATCGCCTATGCCAGGTTATTACCTTGATTC
GTTGGCATGGGAAGACGCCCAGGATGAAAATCGACTGACTAACTTCCTTAATATCTTCAATCACAGCTCTATTGTCATGC
TCCATCAGATCTGGCGGAAATACCGTTATTACATCTGTTTTGACAAAAACGGACAAGACGATTTTTCTCAGCGTATGTTT
GCTTTGGTGGGACTTGGCAGTAATGTGAACCGTTCTCATTTAAATATCAACCATAGCAAAATGCTGGCCTATGCCGGCTT
ATTAGCCAGCCCCGGTCGATCACCGGAAGTGATATGCAGCTTGGTTTCACACTGTTTTGATCTACCGGATGTTACGGTAC
ACGGCTGGCAAATCAGAAAAATCGCTATCGAGCCATCACAGCAAAACCGTTTAGGTGGAATGCTGAAAATAAGGGGACAG
AAACAAAAAGTTAAACAGTCTGTAATAGGAAAAAACTTTTTCCTCGGTTCATGGGTGAGAAGCTGCAATAGTAAATTTAT
TTTATGTATCAATAATCTCTCTCGTGAACACTTCTTGTCATTTCTCCCAGACGGCAAGAATTTCCTGCCATTGGTAATGT
TTCTCTGTTTTGTCCTGCGTGATCAGATGGCTTGGGATCTGCGTCTTGGCTTGGCCGAAAACCAAGCGGGCGGCATGGTA
CTTGGCACGAAATTAAACAATAACCTAGGGTGGACCAGCTTTCTTGGCGAACCGGAAAAGAAACCCTTTATCACGATTTC
AGTGAATGGATAA					