SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000429
Component protein type	TssL
T6SS ID (Type)	T6SS00050 (Type i1)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (257 a.a.); Nucleotide sequence (771 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS01770
Coordinate (Strand)	391509..392279 (-)
NCBI ID	WP_011144753.1
RefSeq	NC_005126
Uniprot ID	UPI00001D2E80
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	8.5e-60	36..237

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-215	MKTETINGNPIPTQEVTDNAVHLQYQLPLRGESLNPMIDAATPLLGMVMRLKDMGEQPLPDQLYQQVVTDIQAIEQLLQTKGYEPGAIISFRYTLCTFIDETALGHGWNSKNEWLQQSLLVRFHNETWGGEKVFQLLDRLMGEPQRYQHLLEFIYLCFCLGYRGRYKVASQKSDDFERLFRRLHQQLHALRGEAPSIILHNNANERNDRYRLGKR
Transmembrane helix	216-238	LTIKHLFWSGITLLAIVYSFYAI
Outside	239-256	RLHNQTQNILQQLNNLLS

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9929	-	-

Protein sequence: 257 a.a.

						>T6CP000429 NC_005126:c392279-391509 [Photorhabdus laumondii subsp. laumondii TTO1] [TssL]
MKTETINGNPIPTQEVTDNAVHLQYQLPLRGESLNPMIDAATPLLGMVMRLKDMGEQPLPDQLYQQVVTDIQAIEQLLQT
KGYEPGAIISFRYTLCTFIDETALGHGWNSKNEWLQQSLLVRFHNETWGGEKVFQLLDRLMGEPQRYQHLLEFIYLCFCL
GYRGRYKVASQKSDDFERLFRRLHQQLHALRGEAPSIILHNNANERNDRYRLGKRLTIKHLFWSGITLLAIVYSFYAIRL
HNQTQNILQQLNNLLS*					

Nucleotide sequence: 771 bp

						>T6CP000429 NC_005126:c392279-391509 [Photorhabdus laumondii subsp. laumondii TTO1] [TssL]
ATGAAAACTGAAACCATAAATGGCAATCCTATTCCGACGCAGGAGGTCACTGATAATGCCGTGCATCTTCAGTATCAGTT
ACCGCTGCGCGGAGAAAGCCTAAACCCGATGATCGATGCCGCTACACCGCTGCTCGGCATGGTCATGCGTCTAAAAGACA
TGGGCGAACAACCGCTGCCGGACCAGCTTTATCAACAGGTCGTCACTGATATTCAGGCAATCGAACAACTTCTACAAACC
AAAGGCTATGAGCCGGGCGCAATTATCTCTTTTCGCTATACGCTCTGTACCTTCATTGATGAAACCGCATTGGGTCATGG
TTGGAACAGCAAGAATGAGTGGTTACAACAATCACTACTGGTTCGATTCCATAATGAAACCTGGGGTGGCGAAAAAGTTT
TTCAGTTGCTAGATCGTTTGATGGGTGAACCTCAGCGCTATCAACATCTGCTGGAGTTTATCTATCTTTGCTTCTGCCTC
GGTTACCGTGGCCGTTATAAAGTTGCTTCGCAGAAAAGTGATGACTTTGAACGTTTATTCCGTCGATTACACCAACAACT
GCACGCGCTGCGGGGAGAAGCTCCTTCCATCATTCTCCACAATAACGCCAATGAGCGTAATGATCGCTATCGCCTGGGTA
AGCGTCTGACTATTAAACATCTGTTCTGGAGCGGTATTACGCTGCTCGCTATCGTTTATAGCTTCTACGCTATCCGTCTG
CACAACCAAACCCAGAACATCTTACAACAGCTAAATAACTTACTGAGCTAG					