SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000427
Component protein type	TssM
T6SS ID (Type)	T6SS00050 (Type i1)
Strain	Photorhabdus laumondii subsp. laumondii TTO1
Replicon	chromosome
Sequence	Protein sequence (1182 a.a.); Nucleotide sequence (3546 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [2] Jurėnas D, Payelleville A, Roghanian M, et al. Photorhabdus antibacterial Rhs polymorphic toxin inhibits translation through ADP-ribosylation of 23S ribosomal RNA. Nucleic Acids Res. 2021 Aug 20;49(14):8384-8395. doi: 10.1093/nar/gkab608. PMID: 34255843

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	PLU_RS01745
Coordinate (Strand)	382431..385976 (-)
NCBI ID	WP_011144748.1
RefSeq	NC_005126
Uniprot ID	Q7N9H7_PHOLL
KEGG ID	plu:plu0359
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	1.3e-69	513..825
IcmF-related_N	PF14331.8	1.9e-68	210..460
IcmF_C	PF06744.14	2.3e-21	1055..1159

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-20	MNWLSLLLNNIKKPARPRLP
Transmembrane helix	21-43	PLKATASLILALLPCLALIWLWW
Outside	44-62	WGPGWQFKQTHPLDTLSAR
Transmembrane helix	63-82	WLATVIILLLALSFIGIKIW
Inside	83-456	RRLRQLEKLKLEGELNTVDPVRADITRQDRYLHHWKAQLQRHLGTRDYLYQRPWYLVMGNTGSGKTALIQAGCRLTDIAAPDTLSGEEALPLHLRCWLGEKAVIIDPDGFLIDQISPPDTGKPQLPARLWQALLVWLTENRQRQPLNGIILTVDIHRLLTDNKAQRERVIAALHLRLQDLRLTLHHPLPFYLVLTKLDRLHGFSAAFQSLDSAQRAQILGVTFSLNARDEQSWRTELAQFWHQWMQQLNAAMPDMMLNKVDISQRSPLFSFTRQIQGLHSYLVPLLEGLLDNSGPAQPGLRGVYLTSSQQIGQMDDLFTQSAAVQYHLGPQAFPTWPVADTVPYFTHALFEAVLLAEPNLAAENRRWLSRHRHT
Transmembrane helix	457-479	LLTFSAVGAAVVLALWSGWHYYY
Outside	480-1181	QQNYRAGDEVLAQANIFLSVPPPTGEDRLGNLQLPLLNPLREATLAYGDYHQKGLLADMGLYQGAAVGPYVENTYLQLLSQRFLPALMNGLLDDLNRAPKGSEEKLAVLRIMRMLEDGSGRHKSLVEHYLQARWSQAFNGQRPLQAQLLTHLDYALDHTDWRAAREAGDQAAISRFAPYQLPLINAEKELSQLSIYQRVYQNLRIKAQDSLPPPLNLRDQIGPGFDRLFMANNDKQLIIPQFLTINGLNNYFVKQDDQLIDLTVMDSWVLNLSHNVQYSDTDRQEIQRQITEQYLGDYTATWRAAMNNLDIRDFTDIPQALSALEQVISGEQPISRALQILSDNTRLPEINETLPAKARQPLRDTPDYRLRVRIHREFAPETAVLEESGDKNSTLQGVYQQLAALHRYLLGIQNAPVPGKAALNAVQQRLEQHNDDPIFDVQQRAKTLPAPLNRWVGALAEQAWRVVMREAIRSLEIEWHDTVVRQYQTYLAGRYPFNPDATEEVPLSEFGRFFRPGGTLDAFYQQNLKPFVENNLTHRADGQQLIRPDVLEQLKRADRIRDTFFSPQNGLGVQYAIEPLSLTGNKRRSLLNLDGQLLDYAHGRSSIVHLIWPNAMRAGVESQLTLIPDAGGKSPRAIRFTGPWAQLRLINSGKLTNVRQDSFDVRFTVDGGDMTYRLYVDESDNPFAGGLFSQFRLPDTLY

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9873	-	-

Protein sequence: 1182 a.a.

						>T6CP000427 NC_005126:c385976-382431 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
MNWLSLLLNNIKKPARPRLPPLKATASLILALLPCLALIWLWWWGPGWQFKQTHPLDTLSARWLATVIILLLALSFIGIK
IWRRLRQLEKLKLEGELNTVDPVRADITRQDRYLHHWKAQLQRHLGTRDYLYQRPWYLVMGNTGSGKTALIQAGCRLTDI
AAPDTLSGEEALPLHLRCWLGEKAVIIDPDGFLIDQISPPDTGKPQLPARLWQALLVWLTENRQRQPLNGIILTVDIHRL
LTDNKAQRERVIAALHLRLQDLRLTLHHPLPFYLVLTKLDRLHGFSAAFQSLDSAQRAQILGVTFSLNARDEQSWRTELA
QFWHQWMQQLNAAMPDMMLNKVDISQRSPLFSFTRQIQGLHSYLVPLLEGLLDNSGPAQPGLRGVYLTSSQQIGQMDDLF
TQSAAVQYHLGPQAFPTWPVADTVPYFTHALFEAVLLAEPNLAAENRRWLSRHRHTLLTFSAVGAAVVLALWSGWHYYYQ
QNYRAGDEVLAQANIFLSVPPPTGEDRLGNLQLPLLNPLREATLAYGDYHQKGLLADMGLYQGAAVGPYVENTYLQLLSQ
RFLPALMNGLLDDLNRAPKGSEEKLAVLRIMRMLEDGSGRHKSLVEHYLQARWSQAFNGQRPLQAQLLTHLDYALDHTDW
RAAREAGDQAAISRFAPYQLPLINAEKELSQLSIYQRVYQNLRIKAQDSLPPPLNLRDQIGPGFDRLFMANNDKQLIIPQ
FLTINGLNNYFVKQDDQLIDLTVMDSWVLNLSHNVQYSDTDRQEIQRQITEQYLGDYTATWRAAMNNLDIRDFTDIPQAL
SALEQVISGEQPISRALQILSDNTRLPEINETLPAKARQPLRDTPDYRLRVRIHREFAPETAVLEESGDKNSTLQGVYQQ
LAALHRYLLGIQNAPVPGKAALNAVQQRLEQHNDDPIFDVQQRAKTLPAPLNRWVGALAEQAWRVVMREAIRSLEIEWHD
TVVRQYQTYLAGRYPFNPDATEEVPLSEFGRFFRPGGTLDAFYQQNLKPFVENNLTHRADGQQLIRPDVLEQLKRADRIR
DTFFSPQNGLGVQYAIEPLSLTGNKRRSLLNLDGQLLDYAHGRSSIVHLIWPNAMRAGVESQLTLIPDAGGKSPRAIRFT
GPWAQLRLINSGKLTNVRQDSFDVRFTVDGGDMTYRLYVDESDNPFAGGLFSQFRLPDTLY*					

Nucleotide sequence: 3546 bp

						>T6CP000427 NC_005126:c385976-382431 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
ATGAACTGGCTTTCTCTGCTGCTCAACAATATCAAAAAACCGGCGAGGCCGCGTCTGCCGCCCCTGAAAGCCACCGCCTC
GCTGATACTGGCCCTCCTGCCCTGTCTGGCCCTCATCTGGCTCTGGTGGTGGGGCCCCGGCTGGCAGTTTAAGCAGACGC
ATCCGCTGGACACCCTGTCTGCCCGCTGGCTGGCGACGGTGATCATCCTCCTGCTGGCCCTGAGTTTTATCGGGATAAAA
ATCTGGCGCCGCCTGCGCCAGCTTGAAAAACTTAAGCTGGAGGGGGAACTGAACACCGTCGATCCGGTGCGCGCCGATAT
CACCCGTCAGGACCGTTATCTCCATCACTGGAAAGCGCAACTGCAACGCCATCTGGGGACCCGGGATTATCTCTACCAGC
GCCCCTGGTACCTGGTGATGGGTAACACCGGCAGCGGCAAAACCGCCCTGATTCAGGCCGGCTGCCGGCTGACTGACATT
GCCGCCCCGGACACCCTGAGCGGGGAAGAGGCGCTGCCCCTGCATCTGCGCTGCTGGCTGGGGGAGAAAGCCGTCATCAT
TGACCCGGACGGGTTCCTTATCGACCAGATATCGCCGCCGGACACCGGCAAACCCCAGCTGCCCGCCCGCCTGTGGCAGG
CGCTGCTCGTCTGGCTCACCGAAAACCGTCAGCGCCAGCCGCTCAACGGCATCATCCTGACGGTGGACATTCACCGCTTA
CTGACCGACAACAAAGCGCAGCGGGAACGGGTTATTGCCGCGCTTCACCTGCGCCTGCAGGATCTGCGATTAACTCTGCA
TCATCCTCTGCCTTTCTATCTGGTGCTGACCAAACTCGACCGGCTGCACGGTTTCAGCGCCGCTTTCCAGTCGCTGGACA
GCGCGCAGCGCGCCCAGATCCTGGGCGTCACCTTTAGCCTGAATGCGCGGGATGAGCAGAGCTGGCGCACTGAGCTGGCC
CAGTTCTGGCACCAGTGGATGCAGCAGCTCAACGCGGCCATGCCGGACATGATGCTCAACAAAGTCGATATCAGTCAGCG
CAGTCCGCTGTTCAGCTTCACCCGCCAGATACAGGGGCTGCACAGCTATCTGGTGCCGCTGCTGGAGGGGCTCCTCGACA
ACAGCGGCCCGGCACAGCCGGGCCTGCGGGGGGTTTATCTCACCTCGTCTCAACAAATCGGCCAGATGGATGACCTGTTT
ACCCAGTCTGCCGCCGTGCAGTATCATCTGGGGCCGCAGGCTTTTCCCACCTGGCCGGTGGCGGACACCGTGCCCTACTT
CACCCACGCGCTGTTTGAAGCGGTCCTGCTGGCCGAACCGAATCTGGCGGCTGAAAACCGCCGGTGGTTAAGCCGCCACC
GCCACACACTGCTGACCTTCTCCGCCGTGGGTGCCGCCGTGGTGCTGGCCCTGTGGAGCGGCTGGCACTATTACTATCAG
CAGAACTACCGGGCCGGTGACGAGGTCCTGGCGCAGGCCAACATCTTCCTGTCGGTGCCGCCGCCCACCGGCGAAGACCG
TCTCGGCAATTTACAGCTTCCCCTGCTCAATCCGCTGCGTGAGGCCACGCTGGCCTACGGGGATTACCATCAGAAAGGGC
TGCTGGCGGATATGGGGCTCTATCAGGGCGCAGCGGTCGGCCCGTATGTGGAAAACACCTACCTGCAACTGCTGTCTCAG
CGCTTCCTGCCGGCCTTAATGAACGGCCTGCTGGATGACCTGAACCGGGCACCGAAAGGCAGCGAAGAAAAACTGGCCGT
CCTGCGTATCATGCGGATGCTGGAAGATGGCAGCGGCCGCCATAAATCACTGGTCGAACACTATCTGCAGGCGCGCTGGA
GTCAGGCGTTTAACGGCCAGCGCCCGTTGCAGGCGCAGTTACTGACGCATCTGGACTATGCGCTGGATCACACCGACTGG
CGGGCCGCACGGGAGGCGGGCGATCAGGCCGCTATCAGCCGCTTTGCACCTTACCAACTGCCGCTCATCAATGCCGAGAA
AGAACTGAGTCAGCTCTCGATTTATCAGCGGGTCTACCAGAACCTGCGCATTAAAGCCCAGGATTCACTGCCGCCGCCCC
TCAACCTGCGCGACCAGATTGGCCCGGGCTTTGACCGCCTCTTTATGGCCAATAATGACAAGCAACTGATTATCCCGCAG
TTCCTGACGATAAACGGCCTGAACAACTACTTCGTCAAACAGGACGACCAGCTGATCGACCTCACCGTCATGGACAGCTG
GGTGCTGAACCTGTCACACAACGTGCAGTACAGCGACACGGACCGGCAGGAAATCCAGCGACAAATCACTGAGCAGTACC
TGGGAGACTACACGGCCACCTGGCGCGCGGCCATGAACAACCTGGATATTCGTGATTTCACCGATATCCCGCAGGCCCTC
AGCGCCCTTGAACAGGTCATCAGCGGCGAACAGCCCATCAGCCGGGCCCTGCAAATCCTGAGCGACAACACCCGTCTGCC
GGAGATTAACGAGACGCTGCCGGCCAAAGCCCGGCAGCCGTTACGTGATACCCCGGATTACCGCCTGCGGGTGCGTATTC
ACCGGGAATTCGCGCCGGAAACCGCGGTGCTGGAGGAGTCCGGGGACAAAAACAGCACCCTGCAGGGGGTTTATCAGCAA
CTGGCGGCCCTGCACCGTTACCTGCTGGGCATTCAGAACGCGCCGGTCCCGGGGAAAGCCGCCCTGAATGCCGTTCAGCA
GCGGCTTGAACAGCATAACGACGACCCGATTTTCGACGTACAGCAGCGGGCGAAAACCCTGCCGGCACCGCTCAACCGCT
GGGTCGGCGCACTGGCGGAACAGGCCTGGCGGGTGGTGATGCGGGAAGCCATCCGTTCGCTGGAAATCGAATGGCACGAC
ACCGTCGTCAGACAGTACCAGACCTACCTGGCCGGCCGCTATCCGTTTAACCCGGACGCGACGGAGGAGGTGCCGCTCAG
TGAGTTCGGGCGGTTCTTCCGGCCCGGCGGCACGCTGGATGCCTTCTATCAGCAGAACCTGAAACCGTTTGTGGAAAACA
ACCTGACCCACCGCGCGGACGGCCAGCAACTTATCCGCCCGGATGTGCTGGAACAGCTGAAACGGGCCGACCGTATCCGC
GACACCTTCTTCTCGCCGCAAAACGGTCTGGGGGTCCAGTATGCCATTGAGCCCCTCAGCCTGACCGGCAATAAACGCCG
CAGCCTGCTCAATCTCGACGGCCAGCTGCTCGATTACGCCCACGGCCGCAGCAGCATTGTGCACCTCATCTGGCCCAACG
CGATGCGGGCCGGGGTGGAAAGCCAGCTCACGCTGATACCGGATGCCGGCGGCAAATCCCCGCGCGCCATCCGCTTCACC
GGCCCGTGGGCCCAGCTGCGCCTGATTAACAGCGGCAAACTGACTAACGTACGTCAGGATTCGTTTGATGTGCGGTTCAC
GGTGGACGGGGGCGACATGACCTACCGTCTCTACGTGGATGAATCCGACAACCCGTTTGCCGGCGGCCTGTTCAGTCAGT
TCAGACTGCCTGACACCCTGTATTAA					