Detailed information of component protein


Summary

Component ID T6CP000427
Component protein type TssM
T6SS ID (Type) T6SS00050 experimental (Type i1)
Strain Photorhabdus laumondii subsp. laumondii TTO1
Replicon chromosome
Sequence Protein sequence (1182 a.a.); Nucleotide sequence (3546 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) PLU_RS01745
Coordinate (Strand) 382431..385976 (-)
NCBI ID WP_011144748.1
RefSeq NC_005126
Uniprot ID Q7N9H7_PHOLL
KEGG ID plu:plu0359
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
IcmF-related PF06761.14 1.3e-69 513..825
IcmF-related_N PF14331.8 1.9e-68 210..460
IcmF_C PF06744.14 2.3e-21 1055..1159

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Inside 1-20 MNWLSLLLNNIKKPARPRLP
Transmembrane helix 21-43 PLKATASLILALLPCLALIWLWW
Outside 44-62 WGPGWQFKQTHPLDTLSAR
Transmembrane helix 63-82 WLATVIILLLALSFIGIKIW
Inside 83-456 RRLRQLEKLKLEGELNTVDPVRADITRQDRYLHHWKAQLQRHLGTRDYLYQRPWYLVMGNTGSGKTALIQAGCRLTDIAAPDTLSGEEALPLHLRCWLGEKAVIIDPDGFLIDQISPPDTGKPQLPARLWQALLVWLTENRQRQPLNGIILTVDIHRLLTDNKAQRERVIAALHLRLQDLRLTLHHPLPFYLVLTKLDRLHGFSAAFQSLDSAQRAQILGVTFSLNARDEQSWRTELAQFWHQWMQQLNAAMPDMMLNKVDISQRSPLFSFTRQIQGLHSYLVPLLEGLLDNSGPAQPGLRGVYLTSSQQIGQMDDLFTQSAAVQYHLGPQAFPTWPVADTVPYFTHALFEAVLLAEPNLAAENRRWLSRHRHT
Transmembrane helix 457-479 LLTFSAVGAAVVLALWSGWHYYY
Outside 480-1181 QQNYRAGDEVLAQANIFLSVPPPTGEDRLGNLQLPLLNPLREATLAYGDYHQKGLLADMGLYQGAAVGPYVENTYLQLLSQRFLPALMNGLLDDLNRAPKGSEEKLAVLRIMRMLEDGSGRHKSLVEHYLQARWSQAFNGQRPLQAQLLTHLDYALDHTDWRAAREAGDQAAISRFAPYQLPLINAEKELSQLSIYQRVYQNLRIKAQDSLPPPLNLRDQIGPGFDRLFMANNDKQLIIPQFLTINGLNNYFVKQDDQLIDLTVMDSWVLNLSHNVQYSDTDRQEIQRQITEQYLGDYTATWRAAMNNLDIRDFTDIPQALSALEQVISGEQPISRALQILSDNTRLPEINETLPAKARQPLRDTPDYRLRVRIHREFAPETAVLEESGDKNSTLQGVYQQLAALHRYLLGIQNAPVPGKAALNAVQQRLEQHNDDPIFDVQQRAKTLPAPLNRWVGALAEQAWRVVMREAIRSLEIEWHDTVVRQYQTYLAGRYPFNPDATEEVPLSEFGRFFRPGGTLDAFYQQNLKPFVENNLTHRADGQQLIRPDVLEQLKRADRIRDTFFSPQNGLGVQYAIEPLSLTGNKRRSLLNLDGQLLDYAHGRSSIVHLIWPNAMRAGVESQLTLIPDAGGKSPRAIRFTGPWAQLRLINSGKLTNVRQDSFDVRFTVDGGDMTYRLYVDESDNPFAGGLFSQFRLPDTLY



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9873 - -



  Protein sequence: 1182 a.a.    

>T6CP000427 NC_005126:c385976-382431 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
MNWLSLLLNNIKKPARPRLPPLKATASLILALLPCLALIWLWWWGPGWQFKQTHPLDTLSARWLATVIILLLALSFIGIK
IWRRLRQLEKLKLEGELNTVDPVRADITRQDRYLHHWKAQLQRHLGTRDYLYQRPWYLVMGNTGSGKTALIQAGCRLTDI
AAPDTLSGEEALPLHLRCWLGEKAVIIDPDGFLIDQISPPDTGKPQLPARLWQALLVWLTENRQRQPLNGIILTVDIHRL
LTDNKAQRERVIAALHLRLQDLRLTLHHPLPFYLVLTKLDRLHGFSAAFQSLDSAQRAQILGVTFSLNARDEQSWRTELA
QFWHQWMQQLNAAMPDMMLNKVDISQRSPLFSFTRQIQGLHSYLVPLLEGLLDNSGPAQPGLRGVYLTSSQQIGQMDDLF
TQSAAVQYHLGPQAFPTWPVADTVPYFTHALFEAVLLAEPNLAAENRRWLSRHRHTLLTFSAVGAAVVLALWSGWHYYYQ
QNYRAGDEVLAQANIFLSVPPPTGEDRLGNLQLPLLNPLREATLAYGDYHQKGLLADMGLYQGAAVGPYVENTYLQLLSQ
RFLPALMNGLLDDLNRAPKGSEEKLAVLRIMRMLEDGSGRHKSLVEHYLQARWSQAFNGQRPLQAQLLTHLDYALDHTDW
RAAREAGDQAAISRFAPYQLPLINAEKELSQLSIYQRVYQNLRIKAQDSLPPPLNLRDQIGPGFDRLFMANNDKQLIIPQ
FLTINGLNNYFVKQDDQLIDLTVMDSWVLNLSHNVQYSDTDRQEIQRQITEQYLGDYTATWRAAMNNLDIRDFTDIPQAL
SALEQVISGEQPISRALQILSDNTRLPEINETLPAKARQPLRDTPDYRLRVRIHREFAPETAVLEESGDKNSTLQGVYQQ
LAALHRYLLGIQNAPVPGKAALNAVQQRLEQHNDDPIFDVQQRAKTLPAPLNRWVGALAEQAWRVVMREAIRSLEIEWHD
TVVRQYQTYLAGRYPFNPDATEEVPLSEFGRFFRPGGTLDAFYQQNLKPFVENNLTHRADGQQLIRPDVLEQLKRADRIR
DTFFSPQNGLGVQYAIEPLSLTGNKRRSLLNLDGQLLDYAHGRSSIVHLIWPNAMRAGVESQLTLIPDAGGKSPRAIRFT
GPWAQLRLINSGKLTNVRQDSFDVRFTVDGGDMTYRLYVDESDNPFAGGLFSQFRLPDTLY*

  Nucleotide sequence: 3546 bp    

>T6CP000427 NC_005126:c385976-382431 [Photorhabdus laumondii subsp. laumondii TTO1] [TssM]
ATGAACTGGCTTTCTCTGCTGCTCAACAATATCAAAAAACCGGCGAGGCCGCGTCTGCCGCCCCTGAAAGCCACCGCCTC
GCTGATACTGGCCCTCCTGCCCTGTCTGGCCCTCATCTGGCTCTGGTGGTGGGGCCCCGGCTGGCAGTTTAAGCAGACGC
ATCCGCTGGACACCCTGTCTGCCCGCTGGCTGGCGACGGTGATCATCCTCCTGCTGGCCCTGAGTTTTATCGGGATAAAA
ATCTGGCGCCGCCTGCGCCAGCTTGAAAAACTTAAGCTGGAGGGGGAACTGAACACCGTCGATCCGGTGCGCGCCGATAT
CACCCGTCAGGACCGTTATCTCCATCACTGGAAAGCGCAACTGCAACGCCATCTGGGGACCCGGGATTATCTCTACCAGC
GCCCCTGGTACCTGGTGATGGGTAACACCGGCAGCGGCAAAACCGCCCTGATTCAGGCCGGCTGCCGGCTGACTGACATT
GCCGCCCCGGACACCCTGAGCGGGGAAGAGGCGCTGCCCCTGCATCTGCGCTGCTGGCTGGGGGAGAAAGCCGTCATCAT
TGACCCGGACGGGTTCCTTATCGACCAGATATCGCCGCCGGACACCGGCAAACCCCAGCTGCCCGCCCGCCTGTGGCAGG
CGCTGCTCGTCTGGCTCACCGAAAACCGTCAGCGCCAGCCGCTCAACGGCATCATCCTGACGGTGGACATTCACCGCTTA
CTGACCGACAACAAAGCGCAGCGGGAACGGGTTATTGCCGCGCTTCACCTGCGCCTGCAGGATCTGCGATTAACTCTGCA
TCATCCTCTGCCTTTCTATCTGGTGCTGACCAAACTCGACCGGCTGCACGGTTTCAGCGCCGCTTTCCAGTCGCTGGACA
GCGCGCAGCGCGCCCAGATCCTGGGCGTCACCTTTAGCCTGAATGCGCGGGATGAGCAGAGCTGGCGCACTGAGCTGGCC
CAGTTCTGGCACCAGTGGATGCAGCAGCTCAACGCGGCCATGCCGGACATGATGCTCAACAAAGTCGATATCAGTCAGCG
CAGTCCGCTGTTCAGCTTCACCCGCCAGATACAGGGGCTGCACAGCTATCTGGTGCCGCTGCTGGAGGGGCTCCTCGACA
ACAGCGGCCCGGCACAGCCGGGCCTGCGGGGGGTTTATCTCACCTCGTCTCAACAAATCGGCCAGATGGATGACCTGTTT
ACCCAGTCTGCCGCCGTGCAGTATCATCTGGGGCCGCAGGCTTTTCCCACCTGGCCGGTGGCGGACACCGTGCCCTACTT
CACCCACGCGCTGTTTGAAGCGGTCCTGCTGGCCGAACCGAATCTGGCGGCTGAAAACCGCCGGTGGTTAAGCCGCCACC
GCCACACACTGCTGACCTTCTCCGCCGTGGGTGCCGCCGTGGTGCTGGCCCTGTGGAGCGGCTGGCACTATTACTATCAG
CAGAACTACCGGGCCGGTGACGAGGTCCTGGCGCAGGCCAACATCTTCCTGTCGGTGCCGCCGCCCACCGGCGAAGACCG
TCTCGGCAATTTACAGCTTCCCCTGCTCAATCCGCTGCGTGAGGCCACGCTGGCCTACGGGGATTACCATCAGAAAGGGC
TGCTGGCGGATATGGGGCTCTATCAGGGCGCAGCGGTCGGCCCGTATGTGGAAAACACCTACCTGCAACTGCTGTCTCAG
CGCTTCCTGCCGGCCTTAATGAACGGCCTGCTGGATGACCTGAACCGGGCACCGAAAGGCAGCGAAGAAAAACTGGCCGT
CCTGCGTATCATGCGGATGCTGGAAGATGGCAGCGGCCGCCATAAATCACTGGTCGAACACTATCTGCAGGCGCGCTGGA
GTCAGGCGTTTAACGGCCAGCGCCCGTTGCAGGCGCAGTTACTGACGCATCTGGACTATGCGCTGGATCACACCGACTGG
CGGGCCGCACGGGAGGCGGGCGATCAGGCCGCTATCAGCCGCTTTGCACCTTACCAACTGCCGCTCATCAATGCCGAGAA
AGAACTGAGTCAGCTCTCGATTTATCAGCGGGTCTACCAGAACCTGCGCATTAAAGCCCAGGATTCACTGCCGCCGCCCC
TCAACCTGCGCGACCAGATTGGCCCGGGCTTTGACCGCCTCTTTATGGCCAATAATGACAAGCAACTGATTATCCCGCAG
TTCCTGACGATAAACGGCCTGAACAACTACTTCGTCAAACAGGACGACCAGCTGATCGACCTCACCGTCATGGACAGCTG
GGTGCTGAACCTGTCACACAACGTGCAGTACAGCGACACGGACCGGCAGGAAATCCAGCGACAAATCACTGAGCAGTACC
TGGGAGACTACACGGCCACCTGGCGCGCGGCCATGAACAACCTGGATATTCGTGATTTCACCGATATCCCGCAGGCCCTC
AGCGCCCTTGAACAGGTCATCAGCGGCGAACAGCCCATCAGCCGGGCCCTGCAAATCCTGAGCGACAACACCCGTCTGCC
GGAGATTAACGAGACGCTGCCGGCCAAAGCCCGGCAGCCGTTACGTGATACCCCGGATTACCGCCTGCGGGTGCGTATTC
ACCGGGAATTCGCGCCGGAAACCGCGGTGCTGGAGGAGTCCGGGGACAAAAACAGCACCCTGCAGGGGGTTTATCAGCAA
CTGGCGGCCCTGCACCGTTACCTGCTGGGCATTCAGAACGCGCCGGTCCCGGGGAAAGCCGCCCTGAATGCCGTTCAGCA
GCGGCTTGAACAGCATAACGACGACCCGATTTTCGACGTACAGCAGCGGGCGAAAACCCTGCCGGCACCGCTCAACCGCT
GGGTCGGCGCACTGGCGGAACAGGCCTGGCGGGTGGTGATGCGGGAAGCCATCCGTTCGCTGGAAATCGAATGGCACGAC
ACCGTCGTCAGACAGTACCAGACCTACCTGGCCGGCCGCTATCCGTTTAACCCGGACGCGACGGAGGAGGTGCCGCTCAG
TGAGTTCGGGCGGTTCTTCCGGCCCGGCGGCACGCTGGATGCCTTCTATCAGCAGAACCTGAAACCGTTTGTGGAAAACA
ACCTGACCCACCGCGCGGACGGCCAGCAACTTATCCGCCCGGATGTGCTGGAACAGCTGAAACGGGCCGACCGTATCCGC
GACACCTTCTTCTCGCCGCAAAACGGTCTGGGGGTCCAGTATGCCATTGAGCCCCTCAGCCTGACCGGCAATAAACGCCG
CAGCCTGCTCAATCTCGACGGCCAGCTGCTCGATTACGCCCACGGCCGCAGCAGCATTGTGCACCTCATCTGGCCCAACG
CGATGCGGGCCGGGGTGGAAAGCCAGCTCACGCTGATACCGGATGCCGGCGGCAAATCCCCGCGCGCCATCCGCTTCACC
GGCCCGTGGGCCCAGCTGCGCCTGATTAACAGCGGCAAACTGACTAACGTACGTCAGGATTCGTTTGATGTGCGGTTCAC
GGTGGACGGGGGCGACATGACCTACCGTCTCTACGTGGATGAATCCGACAACCCGTTTGCCGGCGGCCTGTTCAGTCAGT
TCAGACTGCCTGACACCCTGTATTAA