SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000402
Component protein type	TssL
T6SS ID (Type)	T6SS00047 (Type i1)
Strain	Helicobacter hepaticus ATCC 51449
Replicon	chromosome
Sequence	Protein sequence (263 a.a.); Nucleotide sequence (789 bp)
Reference	[1] Bartonickova L, Sterzenbach T, Nell S, et al. Hcp and VgrG1 are secreted components of the Helicobacter hepaticus type VI secretion system and VgrG1 increases the bacterial colitogenic potential. Cell Microbiol. 2013 Jun;15(6):992-1011. doi: 10.1111/cmi.12094. Epub 2013 Jan 17. PMID: 23278999 [2] Chow J, Mazmanian SK. A pathobiont of the microbiota balances host colonization and intestinal inflammation. Cell Host Microbe. 2010 Apr 22;7(4):265-276. doi: 10.1016/j.chom.2010.03.004. PMID: 20413095 [3] Ge Z, Sterzenbach T, Whary MT, et al. Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn mice. Microbes Infect. 2008 Jun;10(7):726-33. doi: 10.1016/j.micinf.2008.03.011. Epub 2008 Apr 4. PMID: 18538610

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HH_RS01245
Coordinate (Strand)	242444..243232 (+)
NCBI ID	WP_011115095.1
RefSeq	NC_004917
Uniprot ID	Q7VJJ2_HELHP
KEGG ID	hhe:HH_0251
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	8.4e-45	27..236

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-212	MNTEQELSLLLETNFANHCNNMQNNKILHLTLPLLLFATRLSKTGILDDSYITNTEETFANEILSIGSNLNALRCYEDKDLIKLRYCLCVFIDEMLLHNESFINSHFAKHTLTIRLFDEMLGGDKFYGIANQWLLTPSKHKDMLEFIYTCLILGYQGKYASDTQGKQKINHLCCNIANSLAPAMGSSEGKAFELALNHQEQTRILKLLHKKY
Transmembrane helix	213-235	LRPFWLMAIICSIVALFFLFSFL
Inside	236-262	KLESQKAITQDTLKQNLQHFKVSPHED

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9824	-	-

Protein sequence: 263 a.a.

						>T6CP000402 NC_004917:242444-243232 [Helicobacter hepaticus ATCC 51449] [TssL]
MNTEQELSLLLETNFANHCNNMQNNKILHLTLPLLLFATRLSKTGILDDSYITNTEETFANEILSIGSNLNALRCYEDKD
LIKLRYCLCVFIDEMLLHNESFINSHFAKHTLTIRLFDEMLGGDKFYGIANQWLLTPSKHKDMLEFIYTCLILGYQGKYA
SDTQGKQKINHLCCNIANSLAPAMGSSEGKAFELALNHQEQTRILKLLHKKYLRPFWLMAIICSIVALFFLFSFLKLESQ
KAITQDTLKQNLQHFKVSPHED*					

Nucleotide sequence: 789 bp

						>T6CP000402 NC_004917:242444-243232 [Helicobacter hepaticus ATCC 51449] [TssL]
ATGAACACAGAGCAAGAACTTTCCCTGCTACTTGAAACAAATTTTGCAAACCATTGCAACAATATGCAAAACAATAAGAT
TTTACACTTGACCTTGCCATTACTTTTGTTTGCTACACGACTTTCCAAAACAGGCATACTTGATGATAGCTATATCACAA
ATACAGAAGAAACTTTTGCTAATGAAATCCTCTCTATCGGAAGCAATCTCAATGCTTTGCGTTGTTATGAAGATAAGGAT
TTAATCAAACTCCGTTACTGCTTGTGTGTGTTTATTGATGAAATGCTCTTACATAATGAAAGCTTTATCAATAGCCACTT
TGCAAAACATACACTTACTATACGTCTTTTTGATGAAATGCTAGGTGGAGATAAGTTTTATGGTATTGCCAATCAATGGC
TTCTTACCCCAAGCAAACACAAAGATATGCTAGAATTTATCTATACATGCCTTATTTTGGGCTATCAAGGCAAATATGCC
TCCGACACTCAAGGCAAACAAAAAATTAATCATCTATGCTGCAATATAGCAAATTCTCTTGCTCCTGCTATGGGAAGTAG
CGAAGGAAAAGCTTTTGAATTAGCCCTCAATCATCAAGAGCAAACGCGTATTCTAAAACTTTTGCATAAAAAATACTTGC
GACCATTTTGGCTTATGGCTATCATTTGCTCCATCGTTGCATTATTCTTTCTTTTCTCATTTTTAAAGCTAGAATCTCAA
AAAGCCATTACACAAGATACCCTTAAACAAAATTTGCAACACTTCAAGGTATCTCCACATGAGGATTAA					