SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000400
Component protein type	TssJ
T6SS ID (Type)	T6SS00047 (Type i1)
Strain	Helicobacter hepaticus ATCC 51449
Replicon	chromosome
Sequence	Protein sequence (179 a.a.); Nucleotide sequence (537 bp)
Reference	[1] Bartonickova L, Sterzenbach T, Nell S, et al. Hcp and VgrG1 are secreted components of the Helicobacter hepaticus type VI secretion system and VgrG1 increases the bacterial colitogenic potential. Cell Microbiol. 2013 Jun;15(6):992-1011. doi: 10.1111/cmi.12094. Epub 2013 Jan 17. PMID: 23278999 [2] Chow J, Mazmanian SK. A pathobiont of the microbiota balances host colonization and intestinal inflammation. Cell Host Microbe. 2010 Apr 22;7(4):265-276. doi: 10.1016/j.chom.2010.03.004. PMID: 20413095 [3] Ge Z, Sterzenbach T, Whary MT, et al. Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn mice. Microbes Infect. 2008 Jun;10(7):726-33. doi: 10.1016/j.micinf.2008.03.011. Epub 2008 Apr 4. PMID: 18538610

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HH_RS01235
Coordinate (Strand)	240524..241060 (+)
NCBI ID	WP_011115093.1
RefSeq	NC_004917
Uniprot ID	Q7VJJ4_HELHP
KEGG ID	hhe:HH_0249
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	6.3e-15	35..140

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-3	MRY
Transmembrane helix	4-26	IQLYISFCILACFMGLAISGCSS
Inside	27-178	PVKIQAFNQADSNLNNRGDNVPVTLIIYQLKDISKFKQSSIQDLSTRGNVVLGRDIVDFIKVQVPPNEIDVPVAEFAKKEGKYIGVLALFANPQDKKQKFYKKLNRVFSNKIELNIATEGIMQHKKSKQSQGKDTKADGTAGENNIKDLFNE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9976	pos: 23-24. ISG-CS.	MRYIQLYISFCILACFMGLAISG

Protein sequence: 179 a.a.

						>T6CP000400 NC_004917:240524-241060 [Helicobacter hepaticus ATCC 51449] [TssJ]
MRYIQLYISFCILACFMGLAISGCSSPVKIQAFNQADSNLNNRGDNVPVTLIIYQLKDISKFKQSSIQDLSTRGNVVLGR
DIVDFIKVQVPPNEIDVPVAEFAKKEGKYIGVLALFANPQDKKQKFYKKLNRVFSNKIELNIATEGIMQHKKSKQSQGKD
TKADGTAGENNIKDLFNE*					

Nucleotide sequence: 537 bp

						>T6CP000400 NC_004917:240524-241060 [Helicobacter hepaticus ATCC 51449] [TssJ]
ATGCGATATATCCAGCTTTATATTTCCTTTTGCATTCTCGCTTGCTTTATGGGGCTAGCTATTAGTGGGTGCAGCTCACC
TGTTAAGATACAGGCTTTCAATCAGGCAGATTCTAACCTTAATAATCGTGGCGATAATGTGCCTGTTACGCTCATTATTT
ATCAGCTCAAGGATATTAGTAAATTCAAGCAATCTTCCATACAAGATTTAAGCACACGTGGAAATGTTGTCCTTGGAAGG
GACATTGTAGATTTTATCAAGGTGCAAGTGCCGCCAAATGAAATTGACGTGCCTGTTGCAGAATTTGCAAAAAAAGAGGG
TAAATATATCGGCGTGTTAGCTCTTTTTGCCAATCCGCAGGATAAAAAACAAAAATTCTATAAAAAGCTGAATCGTGTCT
TTTCAAACAAAATCGAGCTTAATATTGCCACTGAAGGCATTATGCAACACAAAAAAAGTAAGCAAAGTCAAGGTAAAGAT
ACAAAGGCTGATGGCACTGCTGGAGAAAACAATATAAAGGATTTGTTCAATGAATAG					