SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000399
Component protein type	TssB
T6SS ID (Type)	T6SS00047 (Type i1)
Strain	Helicobacter hepaticus ATCC 51449
Replicon	chromosome
Sequence	Protein sequence (166 a.a.); Nucleotide sequence (498 bp)
Reference	[1] Bartonickova L, Sterzenbach T, Nell S, et al. Hcp and VgrG1 are secreted components of the Helicobacter hepaticus type VI secretion system and VgrG1 increases the bacterial colitogenic potential. Cell Microbiol. 2013 Jun;15(6):992-1011. doi: 10.1111/cmi.12094. Epub 2013 Jan 17. PMID: 23278999 [2] Chow J, Mazmanian SK. A pathobiont of the microbiota balances host colonization and intestinal inflammation. Cell Host Microbe. 2010 Apr 22;7(4):265-276. doi: 10.1016/j.chom.2010.03.004. PMID: 20413095 [3] Ge Z, Sterzenbach T, Whary MT, et al. Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn mice. Microbes Infect. 2008 Jun;10(7):726-33. doi: 10.1016/j.micinf.2008.03.011. Epub 2008 Apr 4. PMID: 18538610

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HH_RS01230
Coordinate (Strand)	239839..240336 (-)
NCBI ID	WP_011115092.1
RefSeq	NC_004917
Uniprot ID	A0A4U8SIJ7_9HELI, Q7VJJ5_HELHP
KEGG ID	hhe:HH_0248
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VipA	PF05591.14	5.4e-53	6..154

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-165	MAENSTPPKERINITYRSKTNGQNPDVELPLKLMVMGNFTGGNDVPFDEREVVSINKHNFNQVMQKMNIGTHVSVKNVLKQDADELSVNLKFEGLGDFSPDSIATQVPELNKLLQLREALVALKGPMGNIPDFRKALLEALKDKDTKEKLLLEIKENQSTDENKK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9912	-	-

Protein sequence: 166 a.a.

						>T6CP000399 NC_004917:c240336-239839 [Helicobacter hepaticus ATCC 51449] [TssB]
MAENSTPPKERINITYRSKTNGQNPDVELPLKLMVMGNFTGGNDVPFDEREVVSINKHNFNQVMQKMNIGTHVSVKNVLK
QDADELSVNLKFEGLGDFSPDSIATQVPELNKLLQLREALVALKGPMGNIPDFRKALLEALKDKDTKEKLLLEIKENQST
DENKK*					

Nucleotide sequence: 498 bp

						>T6CP000399 NC_004917:c240336-239839 [Helicobacter hepaticus ATCC 51449] [TssB]
ATGGCAGAAAATTCTACTCCGCCAAAAGAGCGGATAAATATCACATATCGTTCAAAGACAAATGGGCAGAATCCTGATGT
AGAGCTTCCTTTGAAGCTAATGGTTATGGGTAATTTTACAGGAGGCAATGATGTGCCGTTTGATGAACGAGAGGTTGTGT
CTATTAATAAACACAATTTTAATCAAGTAATGCAAAAAATGAATATAGGCACACATGTAAGTGTAAAAAATGTTCTCAAG
CAAGATGCAGATGAATTATCTGTAAATCTTAAGTTTGAAGGGTTGGGTGATTTTTCACCAGACAGTATTGCTACTCAAGT
GCCAGAGCTAAACAAATTGCTTCAACTACGTGAAGCCCTTGTAGCATTGAAGGGACCAATGGGCAATATTCCAGATTTTA
GAAAAGCACTCTTGGAAGCCCTCAAGGATAAAGATACAAAAGAGAAGCTTTTGCTAGAGATTAAAGAGAATCAATCTACA
GATGAAAATAAAAAGTGA					