SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000397
Component protein type	TssF
T6SS ID (Type)	T6SS00047 (Type i1)
Strain	Helicobacter hepaticus ATCC 51449
Replicon	chromosome
Sequence	Protein sequence (572 a.a.); Nucleotide sequence (1716 bp)
Reference	[1] Bartonickova L, Sterzenbach T, Nell S, et al. Hcp and VgrG1 are secreted components of the Helicobacter hepaticus type VI secretion system and VgrG1 increases the bacterial colitogenic potential. Cell Microbiol. 2013 Jun;15(6):992-1011. doi: 10.1111/cmi.12094. Epub 2013 Jan 17. PMID: 23278999 [2] Chow J, Mazmanian SK. A pathobiont of the microbiota balances host colonization and intestinal inflammation. Cell Host Microbe. 2010 Apr 22;7(4):265-276. doi: 10.1016/j.chom.2010.03.004. PMID: 20413095 [3] Ge Z, Sterzenbach T, Whary MT, et al. Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn mice. Microbes Infect. 2008 Jun;10(7):726-33. doi: 10.1016/j.micinf.2008.03.011. Epub 2008 Apr 4. PMID: 18538610

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HH_RS01215
Coordinate (Strand)	236141..237856 (-)
NCBI ID	WP_011115089.1
RefSeq	NC_004917
Uniprot ID	Q7VJJ8_HELHP
KEGG ID	hhe:HH_0245
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	2.5e-111	5..557

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-571	VKNIHYFRKELDYLHKMREIFVNKYPKLAPFLSHNSNDPDVERIIESLALLTSKIHEELDGNIPLIAESLINILAPNYTNSLPSMCIQDFALKPESKECKVFIPRHTSLKSTPINQVQCEFKTIYDVCLHPLKISDMSLTNEGKQSVFCLDIVSTNKFFKIADMDIHFLQIYLGSEVYSANTLLLWLLCYLQEIVIVCADTLTSFKIPCDSLETTGFDKNESMFSNGDVGFSAFTLLQELLFLPEKFAFVRIKNLEILKECQSEKMTFKFIFKKELPKDTLPKANQFSLFSTPIVNLFSTQTEPILTDHTRNGYRIFIDRAHLNAYSVIQVLKVKAHNSDSGRRILKNYNSFERFEFVDKGNDFYAISNKQDANGEHYKEISIYSNQSRRETLSIDVLCSNNNLPNGLQIGSINEIVGYQDVLTRNLMLPTKSQHRSIDNATLWDFIATLSFNYQSITHKESFLALLHTYSFNIDKPKNLYEIISESLQKIESKPAYNVNGFITLRGMQITLYIDDSKFYCLGEAYKIGLVLAHFFSSFVSINSFCEVSLCCTTSNTSFSYSIIYGNKALL

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9944	-	-

Protein sequence: 572 a.a.

						>T6CP000397 NC_004917:c237856-236141 [Helicobacter hepaticus ATCC 51449] [TssF]
VKNIHYFRKELDYLHKMREIFVNKYPKLAPFLSHNSNDPDVERIIESLALLTSKIHEELDGNIPLIAESLINILAPNYTN
SLPSMCIQDFALKPESKECKVFIPRHTSLKSTPINQVQCEFKTIYDVCLHPLKISDMSLTNEGKQSVFCLDIVSTNKFFK
IADMDIHFLQIYLGSEVYSANTLLLWLLCYLQEIVIVCADTLTSFKIPCDSLETTGFDKNESMFSNGDVGFSAFTLLQEL
LFLPEKFAFVRIKNLEILKECQSEKMTFKFIFKKELPKDTLPKANQFSLFSTPIVNLFSTQTEPILTDHTRNGYRIFIDR
AHLNAYSVIQVLKVKAHNSDSGRRILKNYNSFERFEFVDKGNDFYAISNKQDANGEHYKEISIYSNQSRRETLSIDVLCS
NNNLPNGLQIGSINEIVGYQDVLTRNLMLPTKSQHRSIDNATLWDFIATLSFNYQSITHKESFLALLHTYSFNIDKPKNL
YEIISESLQKIESKPAYNVNGFITLRGMQITLYIDDSKFYCLGEAYKIGLVLAHFFSSFVSINSFCEVSLCCTTSNTSFS
YSIIYGNKALL*					

Nucleotide sequence: 1716 bp

						>T6CP000397 NC_004917:c237856-236141 [Helicobacter hepaticus ATCC 51449] [TssF]
GTGAAAAATATTCATTATTTCCGTAAAGAGCTTGATTATTTGCATAAAATGAGGGAAATTTTTGTTAATAAATATCCCAA
ACTTGCCCCATTTTTATCCCACAATAGTAATGATCCAGATGTGGAACGTATTATAGAATCTTTAGCGTTATTAACATCTA
AGATTCACGAAGAGCTTGATGGCAATATTCCCCTGATTGCAGAATCTTTGATTAACATTCTTGCACCCAATTACACAAAC
TCGTTGCCATCAATGTGCATACAGGACTTTGCACTTAAGCCAGAAAGCAAAGAATGCAAGGTTTTTATTCCTAGACATAC
AAGTCTTAAATCTACTCCAATAAATCAAGTGCAATGTGAATTTAAAACTATCTATGATGTTTGTCTGCATCCTCTTAAAA
TAAGCGATATGTCGCTTACTAACGAAGGGAAGCAGAGTGTATTTTGCCTTGATATTGTAAGCACAAACAAATTTTTTAAA
ATAGCAGATATGGATATTCATTTTTTACAGATTTATCTTGGCAGTGAGGTTTATAGTGCCAATACATTACTTTTATGGCT
TTTATGTTATTTGCAAGAAATTGTTATTGTCTGTGCTGATACTCTCACATCTTTTAAGATTCCTTGTGATTCTTTAGAGA
CTACGGGTTTTGACAAAAATGAGAGTATGTTTAGTAATGGCGATGTAGGCTTTAGTGCTTTTACACTTTTACAAGAATTG
CTTTTTTTGCCAGAAAAATTTGCATTTGTGAGAATAAAAAATCTAGAAATACTAAAAGAATGTCAAAGTGAAAAGATGAC
TTTTAAATTTATTTTCAAAAAAGAATTGCCTAAAGATACCCTTCCAAAAGCCAATCAGTTTTCGCTATTTAGCACGCCCA
TTGTTAATCTTTTTTCTACCCAGACAGAGCCGATTCTTACTGACCATACACGAAATGGGTATAGAATATTTATCGATAGG
GCGCATCTTAACGCATATTCTGTGATACAGGTATTGAAAGTTAAGGCTCATAATAGCGACTCAGGAAGACGGATATTAAA
AAATTACAATAGTTTTGAGCGATTTGAATTTGTAGATAAGGGAAATGATTTTTATGCTATTTCAAATAAGCAAGATGCAA
ATGGAGAGCATTATAAAGAAATTAGTATTTATAGTAATCAATCTCGCAGAGAAACACTTTCCATTGATGTTTTATGTAGC
AACAACAATCTTCCAAATGGTTTGCAGATAGGCTCAATCAATGAGATTGTAGGATATCAAGATGTCCTTACACGTAATCT
TATGTTACCTACAAAATCGCAACATAGAAGTATTGATAATGCTACATTGTGGGATTTTATCGCTACCCTTTCTTTTAATT
ATCAAAGTATAACGCATAAAGAGAGCTTCTTAGCATTGTTGCATACATACAGCTTTAATATTGATAAACCAAAAAATCTT
TACGAAATAATAAGTGAATCCTTACAAAAGATAGAATCCAAGCCTGCTTATAATGTCAATGGATTTATTACATTAAGAGG
TATGCAAATTACCCTATATATTGATGATTCAAAGTTTTATTGTTTAGGTGAGGCATATAAGATAGGCTTAGTGCTTGCAC
ACTTTTTTTCTTCTTTTGTTAGTATCAATTCTTTTTGCGAGGTATCCTTGTGCTGTACAACAAGTAATACATCTTTTAGC
TATTCTATCATCTATGGCAATAAGGCATTATTATGA					