SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000395
Component protein type	TssI
T6SS ID (Type)	T6SS00047 (Type i1)
Strain	Helicobacter hepaticus ATCC 51449
Replicon	chromosome
Sequence	Protein sequence (933 a.a.); Nucleotide sequence (2799 bp)
Reference	[1] Bartonickova L, Sterzenbach T, Nell S, et al. Hcp and VgrG1 are secreted components of the Helicobacter hepaticus type VI secretion system and VgrG1 increases the bacterial colitogenic potential. Cell Microbiol. 2013 Jun;15(6):992-1011. doi: 10.1111/cmi.12094. Epub 2013 Jan 17. PMID: 23278999 [2] Chow J, Mazmanian SK. A pathobiont of the microbiota balances host colonization and intestinal inflammation. Cell Host Microbe. 2010 Apr 22;7(4):265-276. doi: 10.1016/j.chom.2010.03.004. PMID: 20413095 [3] Ge Z, Sterzenbach T, Whary MT, et al. Helicobacter hepaticus HHGI1 is a pathogenicity island associated with typhlocolitis in B6.129-IL10 tm1Cgn mice. Microbes Infect. 2008 Jun;10(7):726-33. doi: 10.1016/j.micinf.2008.03.011. Epub 2008 Apr 4. PMID: 18538610

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	HH_RS09280
Coordinate (Strand)	231618..234416 (-)
NCBI ID	WP_011115086.1
RefSeq	NC_004917
Uniprot ID	UPI00001AB1DD
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
Phage_GPD	PF05954.13	2.2e-22	183..458
Phage_base_V	PF04717.14	2.1e-09	592..639

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-932	MSAFLSLDINSITFTITKAHIRESLQSPFHITCNGFIESFYADIFSATFASLGKTNSPSHTIQSHTQDTDFASSAPPYSFALDQPFNLQLLQDNFALLYISNPYGNTNYHSALSISLGNTSTPIKSYKGIITHIEYLGSKSNVSLDTNNTINTITDITNTSQQKTLSSLDIPTPKSINHRHFFTFTLTSPLIRLSLNKANRIYTDKTPLDILKDILTAYTPQLCKQIDFSSLSQIDNPLEIITQYEESDLDFITRLAHNNGIYFYEDNDTLYVCHHTPYTPIPLTKVPFNPNVKTHNNLNEPCIFALTHSHHIHTKQFNFFSNDANNPQINTYMYSKYIDKQSPDIATHSTSNDTSLAQEDMNNPYNYTASMQSVFIPNVSFTYQANIKSLCSNQAQHTANTQALMNAQSNIYTLCLAQRLYIESKNKSLLGNNIQDNKTQEFINEYTIIALEQTLINESILADTLPSHQQANDSNISRFKDTYSNLITLLPSNLSYMPTPKPKPNPPSLMQGIVVGEEYIKAKNIKEANASIIKQSNTLYTDNFGRVKVRLHAFMAQALLTDNDIHTTPNTNNTKHSNNTHLEDDLYTLNTHTAFLRVASPIASASSGFYHTPRVGDEVLISYLNNDIDMPIIIGSLYNPSNPALPPLPLNAHQTSLSARTLNNSSALQAENTNSHHNLIESGFNEITLSNLKEQEHIYLQAQRDYEELIKHNFLQTIHNNKESQVEGTYNERIKKIHTQTIDLAKIVSIGGEYNTNVALSKDTIVGLSHTLNVGVDNKVRIAKKSSEYVGEDKEVEIGGNLNTSIKQDESRNVGGNKREVVEGDSDISIEKKFNIQTQGEIAIHSNENIHLSSPQSLSLESDRAAVMVADNITMIADSNYLLNANNEATIQVSDTSIVAKSDSIVIKAGGVEVVIDSKGLVVKGGEVKAE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9794	-	-

Protein sequence: 933 a.a.

						>T6CP000395 NC_004917:c234416-231618 [Helicobacter hepaticus ATCC 51449] [TssI]
MSAFLSLDINSITFTITKAHIRESLQSPFHITCNGFIESFYADIFSATFASLGKTNSPSHTIQSHTQDTDFASSAPPYSF
ALDQPFNLQLLQDNFALLYISNPYGNTNYHSALSISLGNTSTPIKSYKGIITHIEYLGSKSNVSLDTNNTINTITDITNT
SQQKTLSSLDIPTPKSINHRHFFTFTLTSPLIRLSLNKANRIYTDKTPLDILKDILTAYTPQLCKQIDFSSLSQIDNPLE
IITQYEESDLDFITRLAHNNGIYFYEDNDTLYVCHHTPYTPIPLTKVPFNPNVKTHNNLNEPCIFALTHSHHIHTKQFNF
FSNDANNPQINTYMYSKYIDKQSPDIATHSTSNDTSLAQEDMNNPYNYTASMQSVFIPNVSFTYQANIKSLCSNQAQHTA
NTQALMNAQSNIYTLCLAQRLYIESKNKSLLGNNIQDNKTQEFINEYTIIALEQTLINESILADTLPSHQQANDSNISRF
KDTYSNLITLLPSNLSYMPTPKPKPNPPSLMQGIVVGEEYIKAKNIKEANASIIKQSNTLYTDNFGRVKVRLHAFMAQAL
LTDNDIHTTPNTNNTKHSNNTHLEDDLYTLNTHTAFLRVASPIASASSGFYHTPRVGDEVLISYLNNDIDMPIIIGSLYN
PSNPALPPLPLNAHQTSLSARTLNNSSALQAENTNSHHNLIESGFNEITLSNLKEQEHIYLQAQRDYEELIKHNFLQTIH
NNKESQVEGTYNERIKKIHTQTIDLAKIVSIGGEYNTNVALSKDTIVGLSHTLNVGVDNKVRIAKKSSEYVGEDKEVEIG
GNLNTSIKQDESRNVGGNKREVVEGDSDISIEKKFNIQTQGEIAIHSNENIHLSSPQSLSLESDRAAVMVADNITMIADS
NYLLNANNEATIQVSDTSIVAKSDSIVIKAGGVEVVIDSKGLVVKGGEVKAE*					

Nucleotide sequence: 2799 bp

						>T6CP000395 NC_004917:c234416-231618 [Helicobacter hepaticus ATCC 51449] [TssI]
ATGAGTGCTTTTCTTTCTTTGGATATTAACTCTATCACCTTTACCATCACCAAAGCCCATATTAGAGAAAGTTTGCAGTC
TCCTTTTCATATTACTTGTAATGGTTTTATAGAATCCTTTTATGCAGATATATTTTCTGCTACTTTTGCTTCTTTGGGTA
AAACAAACTCTCCCTCACACACAATCCAATCTCATACCCAAGATACCGATTTTGCATCATCTGCTCCCCCTTATTCTTTT
GCATTAGACCAACCTTTCAATCTCCAGCTATTGCAAGATAATTTTGCTCTATTATATATTTCTAATCCCTATGGCAACAC
AAACTATCATAGTGCTCTTTCTATCTCTTTAGGTAATACTTCTACCCCTATTAAATCATATAAGGGTATCATTACACATA
TAGAGTATTTAGGTTCTAAATCTAATGTTTCCTTAGATACAAATAACACAATCAACACTATTACTGACATAACCAATACT
TCTCAACAAAAGACACTCTCTTCCTTAGATATACCTACACCTAAATCTATAAACCATAGACATTTCTTTACCTTTACACT
CACTTCTCCTCTCATAAGACTTTCACTCAATAAAGCAAATAGAATCTATACAGATAAAACACCTTTAGACATACTCAAAG
ATATACTCACTGCTTATACTCCACAATTATGCAAACAAATAGATTTTTCTTCTCTTAGCCAAATTGATAATCCTTTAGAG
ATTATCACCCAATATGAAGAATCTGATTTAGATTTCATCACACGATTAGCTCATAATAATGGTATATACTTTTATGAAGA
CAATGATACCTTATATGTATGTCATCACACACCCTATACTCCTATTCCTCTCACTAAAGTCCCTTTTAATCCTAATGTAA
AAACACATAATAATCTTAATGAACCCTGTATTTTTGCTCTCACACATTCTCATCATATACACACAAAACAATTTAACTTC
TTCAGCAATGATGCAAACAATCCTCAAATCAATACTTATATGTATTCAAAATATATTGACAAACAATCCCCAGATATTGC
CACTCATTCTACATCAAATGATACTTCTCTAGCACAAGAAGATATGAATAATCCTTATAACTATACTGCCTCTATGCAAA
GTGTATTTATACCCAATGTGAGTTTTACCTATCAAGCGAATATAAAGTCTCTTTGCTCTAATCAAGCCCAACATACTGCC
AATACTCAAGCTCTTATGAACGCTCAAAGCAATATCTATACACTCTGTTTAGCTCAAAGATTATATATAGAATCTAAAAA
TAAATCTCTTTTAGGAAATAATATTCAAGATAATAAAACGCAAGAGTTTATAAATGAATATACTATCATAGCATTAGAGC
AAACCCTTATAAACGAATCTATTCTTGCAGATACTTTACCCTCTCATCAACAAGCCAATGATAGCAACATCTCTCGGTTC
AAAGATACTTATAGTAATCTTATTACTCTCTTGCCTTCAAACCTATCTTATATGCCTACACCCAAGCCCAAACCAAACCC
TCCATCTCTTATGCAAGGTATAGTTGTAGGAGAAGAGTATATAAAAGCCAAGAATATTAAAGAGGCTAATGCAAGTATCA
TCAAACAGAGCAACACGCTTTATACAGATAACTTTGGCAGGGTAAAAGTAAGGCTGCATGCCTTTATGGCACAAGCTTTA
CTCACGGATAATGATATACACACAACACCCAATACAAACAATACCAAACATTCAAACAATACTCATTTAGAAGATGATTT
ATATACTCTTAATACACACACAGCTTTTCTTAGAGTAGCCTCTCCTATTGCAAGTGCAAGTTCAGGTTTTTATCATACTC
CAAGAGTGGGAGATGAAGTATTAATAAGTTACTTAAATAATGACATAGATATGCCCATCATTATAGGCAGTCTCTACAAT
CCAAGTAATCCTGCCTTACCTCCCCTCCCTTTAAATGCTCATCAAACAAGTTTAAGTGCAAGAACTCTTAATAATAGCAG
TGCATTGCAAGCAGAGAATACAAACTCTCATCACAATCTCATAGAATCTGGATTCAATGAAATCACGCTCTCCAATCTCA
AAGAACAAGAACACATCTATCTTCAAGCACAAAGAGATTATGAAGAACTCATTAAGCATAACTTTCTCCAAACCATACAT
AACAATAAAGAATCTCAAGTAGAGGGAACTTATAATGAAAGGATTAAAAAGATTCATACTCAAACCATAGATTTAGCAAA
GATTGTAAGTATTGGAGGAGAATATAATACCAATGTAGCTTTATCTAAAGATACCATTGTAGGATTAAGTCATACTTTAA
ATGTAGGAGTAGATAACAAAGTAAGGATTGCAAAGAAGAGTAGTGAATATGTAGGAGAGGATAAGGAGGTGGAGATAGGG
GGGAATCTCAATACAAGTATCAAACAAGATGAGAGTAGGAATGTAGGGGGGAATAAGAGAGAGGTGGTGGAGGGGGACAG
CGATATAAGCATTGAGAAAAAGTTTAATATTCAAACACAAGGTGAAATTGCTATTCATTCTAATGAGAATATTCATTTGT
CATCTCCTCAATCTTTGAGTTTAGAATCTGATAGAGCTGCTGTAATGGTTGCAGACAATATAACAATGATTGCAGATTCT
AATTATCTCCTTAATGCTAATAATGAAGCAACAATTCAAGTGAGCGATACTTCTATTGTAGCCAAAAGTGATTCTATCGT
TATTAAAGCTGGTGGAGTAGAAGTAGTCATAGATTCTAAAGGATTAGTCGTTAAAGGTGGGGAGGTAAAGGCAGAGTGA					