Detailed information of component protein


Summary

Component ID T6CP000356
Component protein type TssH
T6SS ID (Type) T6SS00043 experimental (Type i1)
Strain Vibrio parahaemolyticus RIMD 2210633
Replicon chromosome I
Sequence Protein sequence (891 a.a.); Nucleotide sequence (2673 bp)
Reference
[1] Ma L, Zhang Y, Yan X, et al. Expression of the type VI secretion system 1 component Hcp1 is indirectly repressed by OpaR in Vibrio parahaemolyticus. ScientificWorldJournal. 2012;2012:982140. doi: 10.1100/2012/982140. Epub 2012 Jul 31. PMID: 22924031
[2] Yu Y, Yang H, Li J, et al. Putative type VI secretion systems of Vibrio parahaemolyticus contribute to adhesion to cultured cell monolayers. Arch Microbiol. 2012 Oct;194(10):827-35. Epub 2012 Apr 26. PMID: 22535222
[3] Salomon D, Gonzalez H, Updegraff BL, et al. Vibrio parahaemolyticus type VI secretion system 1 is activated in marine conditions to target bacteria, and is differentially regulated from system 2. PLoS One. 2013 Apr 16;8(4):e61086. doi: 10.1371/journal.pone.0061086. Print 2013. PMID: 23613791
[4] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[5] Salomon D, Kinch LN, Trudgian DC, et al. Marker for type VI secretion system effectors. Proc Natl Acad Sci U S A. 2014 Jun 24;111(25):9271-6. doi: 10.1073/pnas.1406110111. Epub 2014 Jun 9. PMID: 24927539
[6] Salomon D, Klimko JA, Orth K. H-NS regulates the Vibrio parahaemolyticus type VI secretion system 1. Microbiology (Reading). 2014 Sep;160(Pt 9):1867-1873. doi: 10.1099/mic.0.080028-0. Epub 2014 Jul 1. PMID: 24987102
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) VP_RS06765
Coordinate (Strand) 1492844..1495516 (-)
NCBI ID WP_005480775.1
RefSeq NC_004603
Uniprot ID Q87PV8_VIBPA
KEGG ID vpa:VP1392
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 6.2e-44 624..788
AAA_lid_9 PF17871.3 6.1e-31 357..452
ClpB_D2-small PF10431.11 1.5e-13 795..869
AAA PF00004.31 7.4e-10 220..348
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-890 MININLSSLIQRLHPIAKVALEDAAALAVSEKANEVQIEHYLLSLLERPNSDFDVLLSHFDCSENLLRQSVRSTLDTNAKGNGSKPVFSALLIEWLQESWLVSSLDLSETQIRSGALLLTLVSNPLRYGQHGYASILEAVNPDSLKRNFAELTSHSIEAQVATSEKTQAREDGSALSKFTTDFTGKARKGEIDPVFCRDQEIRQIVDILARRRKNNPIAVGEPGVGKTAVVEGLALKIVQGDVPDNLKGVELYGLDMGLLQAGASVKGEFEKRLNAVLDEVKNSPTPIILFIDEAHTLVGGGNQAGGSDAANLLKPALARGEVKTIAATTWSEYKKYFEKDPALARRFQLVKLDEPSPEQAALIIRGLRPAYEKSHNVYVRDDAITAAAALSARYISGRQLPDKAIDVLDTACARVNISLNAIPASVETLQQELAAQQRELEALERDALQQTGDKHSLANIPDLKLAMETTKEELAEQEVQWHKEKEQIQEMIALRSRLHELVFGKVNEEELVVDEDSEQDVSPYTEMDEEAVRIAISACQEQLDAIRNGNPLVHFEVGPDEVSHVISDWTGIPMGKMLQDEAETTLKLKESLTQSIKGQEYAIDALSEGIQTAKAGLGNPDAPTGVFLLVGPSGVGKTETARAIADQMFGGERFMTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKADPEVLNLFYQVFDKGTLNDGEGRTIDFKNTLIIMTSNLATHEIESLVHQSKEIDANIIAEAIRPTLNQHFKPALLARMSVLPFVPLSDEAMTEIIHHKLNKVSQRLHSHHKLSLSYEESLVEFVLGNCRLAETGARNIDAVINRQLLPQLSTQLLVHDKDDSHTQITVSVDEQGTLTYAFS


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9911 - -


  Protein sequence: 891 a.a.    

>T6CP000356 NC_004603:c1495516-1492844 [Vibrio parahaemolyticus RIMD 2210633] [TssH]
MININLSSLIQRLHPIAKVALEDAAALAVSEKANEVQIEHYLLSLLERPNSDFDVLLSHFDCSENLLRQSVRSTLDTNAK
GNGSKPVFSALLIEWLQESWLVSSLDLSETQIRSGALLLTLVSNPLRYGQHGYASILEAVNPDSLKRNFAELTSHSIEAQ
VATSEKTQAREDGSALSKFTTDFTGKARKGEIDPVFCRDQEIRQIVDILARRRKNNPIAVGEPGVGKTAVVEGLALKIVQ
GDVPDNLKGVELYGLDMGLLQAGASVKGEFEKRLNAVLDEVKNSPTPIILFIDEAHTLVGGGNQAGGSDAANLLKPALAR
GEVKTIAATTWSEYKKYFEKDPALARRFQLVKLDEPSPEQAALIIRGLRPAYEKSHNVYVRDDAITAAAALSARYISGRQ
LPDKAIDVLDTACARVNISLNAIPASVETLQQELAAQQRELEALERDALQQTGDKHSLANIPDLKLAMETTKEELAEQEV
QWHKEKEQIQEMIALRSRLHELVFGKVNEEELVVDEDSEQDVSPYTEMDEEAVRIAISACQEQLDAIRNGNPLVHFEVGP
DEVSHVISDWTGIPMGKMLQDEAETTLKLKESLTQSIKGQEYAIDALSEGIQTAKAGLGNPDAPTGVFLLVGPSGVGKTE
TARAIADQMFGGERFMTTINMSEFQEKHTVSRLIGSPPGYVGYGEGGMLTEAVRQRPYSVVLLDEVEKADPEVLNLFYQV
FDKGTLNDGEGRTIDFKNTLIIMTSNLATHEIESLVHQSKEIDANIIAEAIRPTLNQHFKPALLARMSVLPFVPLSDEAM
TEIIHHKLNKVSQRLHSHHKLSLSYEESLVEFVLGNCRLAETGARNIDAVINRQLLPQLSTQLLVHDKDDSHTQITVSVD
EQGTLTYAFS*

  Nucleotide sequence: 2673 bp    

>T6CP000356 NC_004603:c1495516-1492844 [Vibrio parahaemolyticus RIMD 2210633] [TssH]
ATGATAAATATAAACCTTTCTTCATTAATTCAGCGTCTACACCCGATAGCTAAAGTAGCATTAGAAGATGCCGCAGCACT
CGCAGTCTCGGAAAAAGCAAACGAAGTTCAAATTGAGCATTATTTACTGAGTCTATTAGAAAGGCCAAATAGTGATTTTG
ACGTCTTATTAAGTCATTTTGATTGTTCTGAGAACTTATTGAGACAATCGGTTCGATCCACACTCGATACCAACGCCAAA
GGAAATGGCAGCAAACCTGTTTTTTCTGCTTTATTAATCGAATGGTTACAAGAAAGCTGGCTGGTTTCTTCTTTAGATCT
ATCCGAAACACAAATCCGCTCTGGCGCCCTACTCCTTACTCTTGTTAGCAACCCTCTGCGTTATGGCCAACATGGTTACG
CCTCTATTCTTGAAGCAGTAAACCCTGATAGCCTTAAACGTAACTTTGCAGAACTAACAAGCCACTCTATTGAGGCGCAA
GTCGCGACTTCAGAGAAAACACAAGCTCGTGAAGATGGTTCAGCACTGAGCAAGTTCACGACAGACTTCACTGGTAAAGC
ACGCAAAGGCGAAATCGATCCTGTTTTCTGCCGTGACCAAGAAATCCGCCAAATTGTAGACATTCTTGCTCGTCGCCGTA
AAAACAACCCTATTGCCGTTGGTGAGCCGGGCGTTGGTAAAACCGCAGTAGTAGAAGGCTTGGCACTTAAGATTGTACAA
GGTGACGTACCAGACAACCTTAAAGGTGTTGAGCTTTACGGCTTGGACATGGGTCTTCTGCAAGCTGGCGCAAGCGTAAA
AGGCGAATTCGAAAAACGCCTAAATGCAGTATTGGATGAAGTGAAAAACTCGCCAACACCGATCATCTTGTTCATCGATG
AAGCGCACACCCTTGTTGGCGGCGGCAACCAAGCGGGCGGTAGCGATGCTGCTAACTTATTAAAACCTGCCCTAGCACGT
GGTGAAGTAAAGACAATCGCAGCAACAACATGGTCGGAATACAAAAAGTACTTTGAGAAAGATCCAGCTCTTGCGCGTCG
TTTCCAACTGGTGAAACTCGACGAACCGTCTCCTGAGCAAGCAGCATTGATCATTCGTGGTCTGCGCCCGGCGTACGAGA
AATCTCACAATGTTTACGTACGTGACGACGCTATCACCGCAGCGGCAGCGCTTTCTGCTCGATACATCTCTGGTCGCCAA
CTGCCAGACAAAGCGATCGATGTACTAGATACGGCATGTGCACGAGTGAACATCAGCCTTAATGCCATTCCTGCTTCTGT
TGAGACTCTTCAGCAAGAACTTGCAGCTCAACAGCGCGAGCTTGAAGCGCTTGAGCGTGATGCTCTGCAGCAAACTGGCG
ACAAGCACAGCCTTGCTAACATTCCAGACCTAAAATTGGCAATGGAAACGACAAAAGAAGAGCTAGCAGAACAAGAAGTG
CAATGGCACAAAGAAAAAGAGCAGATCCAAGAGATGATTGCTCTACGCTCTCGCTTACATGAACTGGTCTTTGGCAAAGT
AAATGAAGAAGAGCTTGTTGTAGACGAAGATTCAGAACAAGACGTTTCTCCATATACAGAAATGGATGAAGAAGCGGTCC
GCATTGCGATTTCAGCATGCCAAGAACAGTTAGACGCAATCCGCAATGGCAACCCATTAGTTCACTTTGAAGTTGGGCCA
GATGAAGTCAGCCACGTTATCTCAGACTGGACTGGCATTCCAATGGGTAAGATGCTTCAAGACGAAGCAGAAACCACGCT
AAAACTGAAAGAAAGCCTAACACAAAGCATCAAAGGCCAAGAATACGCTATCGATGCACTGTCTGAAGGCATTCAAACCG
CAAAAGCTGGCTTGGGTAATCCAGACGCACCGACAGGCGTATTCCTGCTAGTTGGCCCAAGTGGCGTGGGTAAAACCGAA
ACTGCTCGTGCGATCGCTGATCAAATGTTTGGTGGTGAGCGCTTTATGACGACCATTAACATGTCTGAGTTCCAAGAGAA
GCACACGGTATCTCGCCTAATCGGTTCGCCTCCTGGTTACGTAGGTTACGGTGAAGGCGGCATGCTGACAGAAGCGGTAC
GTCAGCGTCCATATTCTGTTGTACTTCTTGATGAAGTAGAAAAAGCCGATCCAGAAGTGTTGAACCTGTTCTACCAAGTG
TTCGACAAAGGTACGCTGAACGACGGCGAAGGCCGCACCATCGACTTCAAGAACACGCTGATCATCATGACCAGCAACCT
TGCGACGCACGAAATTGAATCTCTGGTTCACCAGTCTAAAGAAATTGATGCTAACATCATCGCAGAAGCCATCCGCCCTA
CTTTGAACCAACACTTCAAGCCGGCGCTGTTGGCACGTATGTCCGTACTTCCGTTTGTGCCTCTGTCTGACGAAGCAATG
ACAGAAATTATCCACCACAAACTAAACAAAGTTTCTCAGCGCCTACATAGCCATCACAAGTTGTCCCTGAGCTACGAAGA
AAGCTTGGTCGAGTTCGTACTAGGTAACTGTCGCCTTGCTGAAACAGGCGCACGTAACATTGACGCGGTCATCAACCGTC
AGTTACTACCTCAGCTATCGACACAGCTATTGGTGCATGATAAGGATGATTCACACACGCAAATCACAGTTTCTGTCGAT
GAGCAAGGAACACTGACCTATGCGTTCAGCTAA