Detailed information of component protein


Summary

Component ID T6CP000341
Component protein type TssK
T6SS ID (Type) T6SS00041 insolico (Type i1)
Strain Pseudomonas syringae pv. tomato str. DC3000
Replicon chromosome
Sequence Protein sequence (444 a.a.); Nucleotide sequence (1332 bp)
Reference
[1] Haapalainen M, Mosorin H, Dorati F, et al. Hcp2, a secreted protein of the phytopathogen Pseudomonas syringae pv. tomato DC3000, is required for fitness for competition against bacteria and yeasts. J Bacteriol. 2012 Sep;194(18):4810-22. doi: 10.1128/JB.00611-12. Epub 2012 Jun 29. PMID: 22753062
[2] Sarris PF, Skandalis N, Kokkinidis M, et al. In silico analysis reveals multiple putative type VI secretion systems and effector proteins in Pseudomonas syringae pathovars. Mol Plant Pathol. 2010 Nov;11(6):795-804. doi: 10.1111/j.1364-3703.2010.00644.x. PMID: 21091602
[3] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603
[4] Barret M, Egan F, Fargier E, et al. Genomic analysis of the type VI secretion systems in Pseudomonas spp.: novel clusters and putative effectors uncovered. Microbiology (Reading). 2011 Jun;157(Pt 6):1726-1739. doi: 10.1099/mic.0.048645-0. Epub 2011 Apr 7. PMID: 21474537
insolico Bioinformatics investigation has been performed on this T6SS
External database links

Locus tag (Gene) PSPTO_RS13210
Coordinate (Strand) 2817825..2819156 (+)
NCBI ID WP_010213774.1
RefSeq NC_004578
Uniprot ID -
KEGG ID -
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS_VasE PF05936.14 3.4e-133 18..441
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-443 MNSHKVIWQEGMLLRPQHFQHNDRYYDHQLKVRTRLAGAFNWGFTALEIDRQFLSSGQIVLNQASGIFPDGSVFDIGDRERPLALDIAPNTSNLSVYVALPIVAGNCIEARSQEQSDVFARFTAYAASVADSNAGEGSSTPVVCARPEFRLLLGEPQGEHTYVMLKLCHILICSPDKAITLDAEYSATFVNAGGSRYLMSCLKEVTSLLQHRGDVIAARISSRGQASDAEVGDFMMLQLINRSALVLRHYLETRHLHPEELYRLLLTLLGELATFGTETRRPRLESSYQHTDQAATFRSVMLAIRQLLSMVVEQHALELELQPRQYGVLVSPRIDGELLGTASFVLAARAQCDAEELRVRLPSHLKVGSVESIRELVALHLPGIRLRPLPVAPRQIPFHANKTYFVLDINDQEQIGIDKSGGFAFHVSGEFSGLELQFWAIRN


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9868 - -


  Protein sequence: 444 a.a.    

>T6CP000341 NC_004578:2817825-2819156 [Pseudomonas syringae pv. tomato str. DC3000] [TssK]
MNSHKVIWQEGMLLRPQHFQHNDRYYDHQLKVRTRLAGAFNWGFTALEIDRQFLSSGQIVLNQASGIFPDGSVFDIGDRE
RPLALDIAPNTSNLSVYVALPIVAGNCIEARSQEQSDVFARFTAYAASVADSNAGEGSSTPVVCARPEFRLLLGEPQGEH
TYVMLKLCHILICSPDKAITLDAEYSATFVNAGGSRYLMSCLKEVTSLLQHRGDVIAARISSRGQASDAEVGDFMMLQLI
NRSALVLRHYLETRHLHPEELYRLLLTLLGELATFGTETRRPRLESSYQHTDQAATFRSVMLAIRQLLSMVVEQHALELE
LQPRQYGVLVSPRIDGELLGTASFVLAARAQCDAEELRVRLPSHLKVGSVESIRELVALHLPGIRLRPLPVAPRQIPFHA
NKTYFVLDINDQEQIGIDKSGGFAFHVSGEFSGLELQFWAIRN*

  Nucleotide sequence: 1332 bp    

>T6CP000341 NC_004578:2817825-2819156 [Pseudomonas syringae pv. tomato str. DC3000] [TssK]
ATGAATTCGCACAAGGTTATCTGGCAGGAAGGCATGCTGCTGCGTCCGCAGCACTTTCAGCATAACGACCGTTATTACGA
TCATCAGTTGAAAGTCCGTACCCGTCTGGCGGGGGCCTTCAACTGGGGGTTCACGGCGCTGGAAATTGATCGGCAATTTC
TGAGCTCTGGGCAGATTGTGCTTAACCAGGCCTCGGGCATATTCCCGGATGGCAGCGTCTTCGATATCGGTGACCGTGAG
CGTCCATTGGCGCTGGATATTGCGCCCAACACTTCAAACCTGTCGGTCTATGTAGCACTGCCGATCGTAGCGGGTAATTG
CATCGAGGCACGCAGCCAGGAACAGTCGGATGTGTTCGCGCGTTTCACCGCCTACGCCGCCAGCGTCGCGGATTCCAACG
CTGGAGAGGGCAGTTCCACACCGGTTGTCTGCGCGCGCCCCGAGTTTCGTCTGCTGCTGGGTGAACCACAAGGCGAGCAC
ACCTACGTGATGCTCAAGTTGTGCCATATCCTGATCTGCAGCCCTGACAAGGCAATAACACTGGACGCTGAATACTCTGC
CACATTCGTCAATGCCGGCGGTTCCCGGTACTTGATGTCGTGCCTGAAGGAGGTCACAAGCCTGTTGCAGCACCGAGGCG
ATGTCATTGCTGCGCGCATCAGTTCGCGCGGCCAGGCCAGCGATGCCGAGGTGGGTGATTTCATGATGTTGCAATTGATC
AATCGCAGTGCGTTGGTGCTTCGGCACTATCTGGAGACTCGACATCTTCACCCTGAAGAGCTGTATCGCTTATTGCTGAC
GCTACTGGGTGAGCTGGCGACGTTTGGCACTGAAACAAGGCGCCCTCGACTGGAAAGCAGCTACCAACATACAGATCAGG
CGGCGACGTTCAGGAGTGTGATGCTTGCCATTCGACAATTGTTGTCGATGGTCGTTGAACAGCATGCGCTGGAACTCGAG
TTGCAGCCCAGGCAGTACGGTGTGTTGGTTTCTCCTCGCATCGACGGTGAGCTGCTGGGAACCGCCTCATTTGTGCTGGC
TGCCCGCGCGCAATGCGATGCCGAAGAACTGCGCGTTCGATTGCCGTCGCACCTCAAGGTCGGTTCTGTGGAGAGTATCC
GTGAACTGGTAGCGTTGCATCTTCCCGGTATCAGGCTCAGGCCGCTGCCCGTGGCACCCCGACAGATCCCGTTTCATGCC
AACAAGACTTACTTCGTTCTCGACATCAACGATCAGGAGCAGATTGGAATCGATAAGTCCGGAGGGTTCGCCTTTCATGT
GTCAGGCGAGTTCTCGGGGCTTGAATTGCAGTTTTGGGCGATCAGAAACTGA