SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000300
Component protein type	TssE
T6SS ID (Type)	T6SS00037 (Type i2)
Strain	Escherichia coli CFT073
Replicon	chromosome
Sequence	Protein sequence (143 a.a.); Nucleotide sequence (429 bp)
Reference	[1] Lloyd AL, Henderson TA, Vigil PD, et al. Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 2009 Jun;191(11):3469-81. doi: 10.1128/JB.01717-08. Epub 2009 Mar 27. PMID: 19329634 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Leiman PG, Basler M, Ramagopal UA, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4154-9. doi: 10.1073/pnas.0813360106. Epub 2009 Feb 27. PMID: 19251641

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C_RS16150
Coordinate (Strand)	3247486..3247914 (+)
NCBI ID	WP_000106967.1
RefSeq	NC_004431
Uniprot ID	S1P1A6_ECOLX, A0A0E2LFQ2_ECOU3, A0A0H2VA70_ECOL6, Q1R7K6_ECOUT, A0A0H2Z217_ECOK1, E2QQS9_ECOLX, B7MLF6_ECO45, A0A1X3K5E6_ECOLX, A0A0H3ENY5_ECO8N
KEGG ID	ecc:c3402, ecv:APECO1_3695, eln:NRG857_13880, ecz:ECS88_3105, eci:UTI89_C3209
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
GPW_gp25	PF04965.16	5.1e-12	31..112

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-142	MSTPSLYEMLTFSFSGELPLEQISERDQLILSVMDNMQRIINCRAGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEETLLRYEPRLSQIQVELLPQPRPGHLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9888	-	-

Protein sequence: 143 a.a.

						>T6CP000300 NC_004431:3247486-3247914 [Escherichia coli CFT073] [TssE]
MSTPSLYEMLTFSFSGELPLEQISERDQLILSVMDNMQRIINCRAGTLAHLPDYGLPDLSLIHQGMAAGIHGLMRQIEET
LLRYEPRLSQIQVELLPQPRPGHLNYLIHAQLPDTGWIRFDGVFSPEGRIVLRHLKQQERAY*					

Nucleotide sequence: 429 bp

						>T6CP000300 NC_004431:3247486-3247914 [Escherichia coli CFT073] [TssE]
ATGTCGACACCTTCCTTATATGAAATGCTGACGTTCAGTTTCAGCGGCGAGTTACCGCTGGAACAGATCAGCGAGCGGGA
TCAGCTTATCCTTTCCGTGATGGACAATATGCAACGCATTATCAACTGCCGGGCAGGCACGCTGGCGCATTTGCCGGATT
ACGGTCTGCCGGATCTCAGTCTGATCCATCAGGGGATGGCTGCGGGTATTCATGGCCTGATGCGTCAGATTGAAGAAACA
CTGCTGCGTTATGAACCACGCCTGAGTCAGATACAGGTGGAATTACTCCCCCAGCCCCGTCCGGGGCATCTTAATTACCT
GATCCACGCGCAGCTTCCCGATACCGGCTGGATACGCTTTGATGGCGTATTTTCTCCGGAAGGACGAATTGTTCTGCGTC
ATCTCAAACAACAGGAGCGGGCGTACTGA					