SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000298
Component protein type	TssM
T6SS ID (Type)	T6SS00037 (Type i2)
Strain	Escherichia coli CFT073
Replicon	chromosome
Sequence	Protein sequence (1113 a.a.); Nucleotide sequence (3339 bp)
Reference	[1] Lloyd AL, Henderson TA, Vigil PD, et al. Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 2009 Jun;191(11):3469-81. doi: 10.1128/JB.01717-08. Epub 2009 Mar 27. PMID: 19329634 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Leiman PG, Basler M, Ramagopal UA, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4154-9. doi: 10.1073/pnas.0813360106. Epub 2009 Feb 27. PMID: 19251641

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C_RS16125
Coordinate (Strand)	3239189..3242527 (+)
NCBI ID	WP_000832727.1
RefSeq	NC_004431
Uniprot ID	A0A0H2VBQ4_ECOL6, A0A2G9AC74_ECOLX
KEGG ID	ecc:c3398
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
IcmF-related	PF06761.14	1.7e-57	397..712
IcmF_C	PF06744.14	1.2e-23	969..1073

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MKRVVR
Transmembrane helix	7-29	LLGVGLLLLVVLLLILFVLAQTT
Outside	30-38	PLISAQDEH
Transmembrane helix	39-61	AVWLRLLITAIVICLLSMCIFFL
Inside	62-342	FSFRQNEASTISLYAQPTDIKEINTEQPNYASLLTIYLRDRYGPFWRRKVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPTAEPDVTLLTALKKLRRSRPLDGIIWALTEEQSRQTAQLDKGWRGLINGGKRLGFQAPLYLWQVCDDGDYQTGRPLQSVGCLLPERCTPEQLAVMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAERGIAHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPHAWLPSPVWAGVTGDNARGRTVG
Transmembrane helix	343-365	FPWLRTALMSAVCVLVIWGAGMT
Outside	366-1112	TSFFANRALVQETGIQTARALDTRLPLAEQLVALHTLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVDHLQQQLNAFVALPPNSPQRTATGEQRYKQLKALLMTSRPEKADAAFFSTTLMADGLRYENIPEGVRQSVLPSLLTFWTANLPEHPQWKTSPPPELTGAVRKILLRQIGVRNAENTLYQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTARREEIDWVLSDRQQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIHWKKEDSLSGILDQLTLMADARQSPLIALTDTLAWQAATGRENRGLSDSLAKSAQELFNGKEKTPQQSREGDDVPVGPLDKTFTPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAPDPQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLTPAADSLNRQWQRAIVSHWNQDFAGRYPFKASQNDASLPLLAQYLRDDGRINLFIAANLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAFASGDAGIHFELRAKPARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGSWSLIRLLEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQVFLTGKSMKDVEEYGEDADE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9777	-	-

Protein sequence: 1113 a.a.

						>T6CP000298 NC_004431:3239189-3242527 [Escherichia coli CFT073] [TssM]
MKRVVRLLGVGLLLLVVLLLILFVLAQTTPLISAQDEHAVWLRLLITAIVICLLSMCIFFLFSFRQNEASTISLYAQPTD
IKEINTEQPNYASLLTIYLRDRYGPFWRRKVRLLLVTGEPEQAEAIAPGLTGQHWLEGDHTVLIYGGRPTAEPDVTLLTA
LKKLRRSRPLDGIIWALTEEQSRQTAQLDKGWRGLINGGKRLGFQAPLYLWQVCDDGDYQTGRPLQSVGCLLPERCTPEQ
LAVMLEAQTLPLTEQGMSQLLTDNRHDFLLRLAHTLAERGIAHWQTVLKPLLAGGAFSSLRLRGLMFSPPLAAVPEAAPH
AWLPSPVWAGVTGDNARGRTVGFPWLRTALMSAVCVLVIWGAGMTTSFFANRALVQETGIQTARALDTRLPLAEQLVALH
TLQGELERLQYRIREGAPWYQRFGLERNQQLLAAAFPGYAQAANRLVRDVAVDHLQQQLNAFVALPPNSPQRTATGEQRY
KQLKALLMTSRPEKADAAFFSTTLMADGLRYENIPEGVRQSVLPSLLTFWTANLPEHPQWKTSPPPELTGAVRKILLRQI
GVRNAENTLYQNVLQQVSRNYADMTLADMTGDTLTESLFSTEQTVPGMFTRQAWEGQVREAIEQVVTARREEIDWVLSDR
QQDTSADISPDTLRNRLTSRYFTDFAGSWLAFLNSIHWKKEDSLSGILDQLTLMADARQSPLIALTDTLAWQAATGRENR
GLSDSLAKSAQELFNGKEKTPQQSREGDDVPVGPLDKTFTPLLRLLGDKAGGGDSQLSLQTYLTRVTRVRLKLQQVTNAP
DPQEMTQQLAQTVLQGKTVDLTDTRDYGRLIAASLGEEWSGFGQALFVRPVEQSWRQVLTPAADSLNRQWQRAIVSHWNQ
DFAGRYPFKASQNDASLPLLAQYLRDDGRINLFIAANLSGVLKREGRYWVADAMNTQGLTVNPDFIRALNRLRDVADTAF
ASGDAGIHFELRAKPARDVMKTHLVIDGQELEYFNQKERWQRFNWPDEQWQPGASLSWTSTQAMERILADYRGSWSLIRL
LEQAQVTPVDSSTFKVVWKAQDGLPLNYLLRVEQGKGPLALLELKNFRLPGQVFLTGKSMKDVEEYGEDADE*					

Nucleotide sequence: 3339 bp

						>T6CP000298 NC_004431:3239189-3242527 [Escherichia coli CFT073] [TssM]
ATGAAAAGAGTTGTGCGTCTTTTGGGTGTGGGGTTACTGCTCCTTGTTGTGTTGTTGCTCATTTTGTTTGTTCTGGCTCA
GACCACACCGCTGATATCAGCACAGGATGAGCATGCTGTCTGGCTTCGTCTGTTGATAACAGCGATTGTGATCTGTTTGC
TAAGTATGTGCATATTTTTCCTCTTTTCTTTCCGGCAGAACGAAGCCTCGACGATATCACTATACGCTCAACCGACTGAT
ATAAAGGAAATAAATACGGAGCAGCCGAACTATGCATCACTGCTGACGATATATTTACGCGACCGCTACGGTCCGTTCTG
GCGGCGTAAAGTCCGCCTGCTGCTGGTGACCGGCGAGCCTGAACAGGCAGAAGCCATCGCGCCGGGGCTGACCGGGCAAC
ACTGGCTGGAAGGCGACCACACGGTGCTGATATATGGCGGCAGGCCAACAGCGGAGCCTGATGTCACACTGCTGACCGCC
TTAAAAAAACTGCGCCGCAGCCGTCCGCTGGACGGCATCATCTGGGCGCTGACAGAAGAACAGAGCCGCCAGACAGCGCA
ACTCGACAAAGGCTGGCGCGGACTGATAAACGGCGGTAAGCGACTCGGTTTTCAGGCTCCACTCTATTTGTGGCAGGTCT
GTGACGACGGTGATTATCAGACCGGACGCCCCCTGCAAAGCGTCGGCTGCCTGCTGCCGGAACGCTGTACCCCGGAACAA
CTGGCTGTAATGCTGGAAGCACAAACCCTGCCGCTGACGGAACAGGGCATGTCGCAGCTACTGACCGATAACCGCCATGA
TTTTCTGCTGCGTCTGGCTCATACCCTTGCAGAGCGGGGTATTGCTCACTGGCAGACCGTCCTGAAACCGCTGCTGGCAG
GCGGCGCATTTTCTTCCCTGCGCCTGCGCGGCCTGATGTTCAGCCCGCCGCTTGCCGCCGTGCCGGAGGCCGCACCTCAT
GCGTGGCTGCCGTCACCGGTCTGGGCGGGCGTGACGGGTGATAACGCGCGCGGGCGCACGGTGGGTTTTCCGTGGCTGCG
TACCGCACTGATGTCCGCTGTCTGCGTGCTGGTGATATGGGGGGCCGGAATGACGACCTCGTTCTTCGCCAACCGCGCTC
TTGTTCAGGAAACCGGTATCCAGACGGCACGTGCGCTTGATACCCGCCTGCCGCTGGCAGAACAACTGGTGGCGCTGCAT
ACCCTGCAGGGCGAACTGGAACGCCTGCAATATCGTATCCGCGAAGGTGCGCCGTGGTATCAGCGTTTTGGCCTTGAACG
TAACCAACAACTGCTCGCCGCCGCTTTTCCCGGCTATGCGCAGGCGGCAAACCGGCTGGTGCGCGACGTGGCCGTTGACC
ATCTGCAACAGCAACTGAACGCCTTTGTCGCCCTGCCGCCCAACAGTCCTCAGCGTACCGCCACCGGTGAACAACGCTAT
AAGCAGCTTAAGGCATTGCTGATGACTTCCCGCCCGGAAAAGGCCGACGCTGCCTTTTTCAGTACCACGCTGATGGCGGA
CGGTCTGCGCTACGAGAATATCCCGGAAGGTGTGCGGCAGAGCGTGTTGCCGTCACTGCTGACCTTCTGGACGGCGAACC
TGCCGGAACACCCGCAGTGGAAAACATCGCCGCCACCGGAACTGACCGGCGCAGTGCGTAAAATCCTGCTGCGCCAGATT
GGTGTGCGTAATGCCGAAAACACCCTCTACCAGAACGTGCTGCAACAGGTGTCCCGCAACTACGCCGATATGACGCTGGC
GGACATGACCGGGGATACCCTCACCGAATCTCTTTTCAGTACGGAACAGACGGTGCCGGGGATGTTCACCCGTCAGGCGT
GGGAAGGACAGGTCAGGGAAGCCATCGAGCAGGTGGTGACGGCGCGGCGCGAGGAAATCGACTGGGTACTCAGCGACCGC
CAGCAGGATACCTCTGCGGATATCTCGCCGGATACGCTGCGTAACCGTCTCACCTCACGCTACTTTACCGACTTTGCCGG
AAGCTGGCTGGCGTTTCTCAACAGCATTCACTGGAAAAAGGAAGACTCGCTCTCCGGCATTCTCGACCAGCTGACACTGA
TGGCCGATGCCCGTCAGTCGCCACTGATTGCGCTGACGGACACCCTCGCGTGGCAGGCGGCGACAGGCAGGGAAAACCGT
GGTCTGTCAGACTCGCTGGCGAAATCGGCACAGGAACTGTTTAACGGCAAGGAGAAAACGCCGCAGCAATCCCGTGAAGG
TGACGACGTGCCTGTCGGGCCGCTGGATAAAACCTTCACGCCGCTGCTGCGTTTGCTGGGCGATAAGGCCGGAGGCGGCG
ACAGCCAGCTGAGTCTACAGACCTACCTCACCCGCGTCACCCGCGTGCGCCTCAAACTGCAACAGGTGACCAACGCCCCC
GACCCGCAGGAGATGACCCAACAACTGGCGCAGACGGTCTTACAGGGTAAAACCGTTGACCTCACCGACACCCGCGACTA
CGGACGGTTAATCGCCGCCAGTCTGGGCGAAGAATGGAGTGGCTTCGGTCAGGCGCTGTTCGTTCGCCCGGTAGAGCAGT
CGTGGCGGCAGGTGCTGACGCCTGCGGCGGACAGCCTGAACCGCCAGTGGCAGCGGGCGATTGTCAGCCACTGGAATCAG
GACTTCGCTGGCCGCTATCCGTTCAAAGCCTCACAGAACGATGCCTCCCTCCCCCTGCTGGCGCAGTACCTGCGCGATGA
CGGGCGCATCAACCTGTTTATCGCCGCCAACCTTTCCGGCGTGCTGAAACGAGAGGGCCGCTACTGGGTGGCTGACGCCA
TGAACACGCAGGGGCTGACGGTCAATCCGGACTTTATCCGCGCCCTGAACCGCCTGCGCGACGTGGCCGATACCGCCTTT
GCCAGCGGCGATGCCGGGATACATTTTGAACTGCGGGCAAAACCGGCGCGTGACGTGATGAAGACGCATCTGGTGATTGA
CGGGCAGGAGCTGGAATATTTCAACCAGAAAGAACGCTGGCAGCGTTTTAACTGGCCGGATGAACAGTGGCAACCCGGCG
CATCGCTAAGCTGGACCAGCACACAGGCGATGGAGCGCATACTGGCGGATTACCGGGGAAGCTGGAGTCTTATTCGCCTG
CTGGAACAGGCGCAGGTGACGCCGGTGGACAGCAGCACCTTTAAGGTGGTGTGGAAAGCGCAGGACGGCCTGCCGCTGAA
TTACCTGCTACGGGTTGAACAGGGTAAAGGGCCGCTGGCGCTGCTGGAGCTGAAAAACTTCCGCCTGCCGGGACAGGTGT
TTCTGACCGGAAAAAGTATGAAGGATGTGGAAGAGTATGGGGAAGACGCCGATGAGTGA					