Detailed information of component protein


Summary

Component ID T6CP000297
Component protein type TssI
T6SS ID (Type) T6SS00037 experimental (Type i2)
Strain Escherichia coli CFT073
Replicon chromosome
Sequence Protein sequence (821 a.a.); Nucleotide sequence (2463 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) C_RS16100
Coordinate (Strand) 3232193..3234655 (+)
NCBI ID WP_001529381.1
RefSeq NC_004431
Uniprot ID A0A234RAP6_ECOLX, A0A0H3EN01_ECO8N
KEGG ID eln:NRG857_13820
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
Phage_GPD PF05954.13 6.4e-59 30..346
DUF2345 PF10106.11 7.1e-35 603..747
T6SS_Vgr PF13296.8 3.8e-34 484..585
Phage_base_V PF04717.14 1.7e-09 397..463
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-820 MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRTLHGVITGFKHLSSSQDEARYEVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQYGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNTAYSVVEKSVSTRDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADNFLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGLEIKVQGDDAPAVFRKGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKDGRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKRNVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKEQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQVLEMQPALARLSAALVTMESLAASAQQAQALAADVSRQQTLLQQKIEQLQQEVLLGSAPKGVALVSGEDMQLSASDNLTLTAGKQLDIGVQKDFTLAAGKQLSLYSRAGAKLFSSHNDIDIQAQGGNLTTWSTQDTYISSGKKLVVTAQDELTLICGGGYIKIKSGNVEIGGPGKLLVKNAGIKKAGSGSMQGVMKSFEPESFDENFIISNPASGKPIANQKYEIHMPDGSVIAGITDAMGYSSLVASKASDDLKIILKK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.9926 - -


  Protein sequence: 821 a.a.    

>T6CP000297 NC_004431:3232193-3234655 [Escherichia coli CFT073] [TssI]
MNVQFFDHAHHKLKIRGLQSPVDVLTFEGREQLSTPFRYDIQFTSSDKAIAPESVLMQDGAFSLTAPPVQGMPVQTALRT
LHGVITGFKHLSSSQDEARYEVRLEPRMALLTRSRQNAIYQNQTVPQIVEKILRERHQMRGQDFVFNLKSEYPAREQVMQ
YGEDDLTFVSRLLSEVGIWFRFATDARLKIEVIEFYDDQSGYERGLTLPLRHPSGLFDGETEAVWGLNTAYSVVEKSVST
RDYNYREATAEMTTGQHDATGGDNTTYGEAYHYADNFLQQGDKEAAESGAFYARIRHERYLNEQAILKGQSTSSLLMPGL
EIKVQGDDAPAVFRKGVLITGVTTSAARDRSYELTFTAIPYSERYGYRPALIPRPVMAGTLPARVTSTVKNDIYAHIDKD
GRYRVNLDFDRDTWKPGYESLWVRQSRPYAGDTYGLHLPLLAGTEVSIAFEEGNPDRPYIAGVKHDSAHTDHVTIQNYKR
NVLRTPANNKIRLDDERGKEHIKVSTEYGGKSQLNLGHLVDAGKEQRGEGFELRTDLWGAVRAKKGIFISADAQDKAQGQ
VLEMQPALARLSAALVTMESLAASAQQAQALAADVSRQQTLLQQKIEQLQQEVLLGSAPKGVALVSGEDMQLSASDNLTL
TAGKQLDIGVQKDFTLAAGKQLSLYSRAGAKLFSSHNDIDIQAQGGNLTTWSTQDTYISSGKKLVVTAQDELTLICGGGY
IKIKSGNVEIGGPGKLLVKNAGIKKAGSGSMQGVMKSFEPESFDENFIISNPASGKPIANQKYEIHMPDGSVIAGITDAM
GYSSLVASKASDDLKIILKK*

  Nucleotide sequence: 2463 bp    

>T6CP000297 NC_004431:3232193-3234655 [Escherichia coli CFT073] [TssI]
ATGAACGTACAGTTCTTCGACCACGCACACCACAAGCTCAAAATTCGCGGCCTGCAAAGTCCGGTGGATGTGCTGACCTT
TGAAGGCCGCGAGCAACTGAGTACCCCGTTCCGCTACGACATTCAGTTTACCAGCAGCGATAAAGCCATCGCGCCGGAGT
CGGTGCTGATGCAGGATGGCGCATTCAGCCTGACGGCACCGCCGGTACAGGGAATGCCGGTACAGACTGCCCTGCGCACC
CTGCACGGCGTCATTACCGGCTTTAAACATCTCTCATCGTCACAGGACGAAGCCCGTTACGAAGTGCGCCTTGAACCCCG
TATGGCGCTGCTTACCCGCAGCCGCCAGAACGCCATCTACCAGAACCAGACGGTGCCGCAAATCGTCGAAAAAATCCTGC
GCGAGCGGCACCAGATGCGCGGTCAGGACTTCGTGTTTAACCTCAAAAGCGAATACCCGGCCCGCGAACAGGTGATGCAG
TACGGTGAGGACGACCTGACGTTTGTTTCACGCCTGCTGTCAGAGGTGGGTATCTGGTTCCGCTTTGCCACCGACGCCCG
GTTGAAAATCGAAGTTATCGAATTTTACGACGACCAGAGCGGCTACGAGCGCGGGCTGACGCTGCCGCTTCGCCATCCTT
CTGGGCTGTTTGATGGTGAAACGGAAGCCGTATGGGGGCTGAACACCGCGTACAGCGTGGTGGAAAAAAGTGTCTCCACG
CGGGACTACAACTACCGTGAAGCCACGGCGGAAATGACCACAGGCCAGCATGACGCCACCGGGGGCGATAACACCACTTA
CGGCGAAGCCTACCACTATGCCGACAACTTCCTGCAACAAGGCGATAAAGAGGCAGCGGAAAGCGGCGCGTTTTATGCCC
GTATCCGTCACGAACGCTACCTGAATGAACAGGCCATTCTCAAAGGCCAGAGCACCAGTTCACTGCTGATGCCGGGGCTG
GAGATAAAAGTTCAGGGCGACGACGCGCCTGCGGTGTTCCGCAAGGGCGTGCTGATTACCGGCGTGACGACCAGCGCGGC
GCGTGACCGCAGCTATGAGCTGACGTTCACGGCGATACCGTACTCAGAACGCTACGGCTACCGTCCGGCGCTAATCCCCC
GCCCGGTCATGGCCGGAACGCTCCCGGCGCGGGTCACCAGCACGGTGAAGAACGATATCTATGCCCATATCGACAAGGAC
GGGCGCTACCGAGTCAACCTTGACTTTGACCGCGATACGTGGAAACCGGGTTATGAAAGCCTGTGGGTACGCCAGTCGCG
CCCGTATGCGGGAGACACCTACGGCCTGCACCTGCCGCTGCTGGCGGGAACGGAGGTCAGCATCGCCTTTGAGGAAGGCA
ACCCGGACAGGCCGTATATCGCCGGGGTAAAACACGACTCGGCGCACACCGACCATGTGACCATTCAGAACTACAAGCGC
AACGTGCTGCGTACCCCGGCCAACAACAAAATCCGGCTGGATGACGAGCGCGGGAAGGAGCATATCAAGGTCAGTACCGA
ATATGGCGGCAAGAGCCAGCTAAACCTCGGCCATCTTGTTGATGCCGGGAAAGAACAGCGTGGCGAAGGCTTTGAACTGC
GTACCGATCTGTGGGGCGCGGTGCGGGCGAAGAAAGGGATTTTTATCAGCGCTGATGCGCAGGATAAGGCGCAGGGTCAG
GTGCTGGAGATGCAGCCAGCTCTGGCTCGCCTTTCTGCAGCGTTGGTGACAATGGAATCTCTGGCTGCCAGTGCGCAACA
GGCTCAGGCACTGGCGGCAGATGTGAGTCGTCAGCAGACGCTGTTGCAGCAAAAAATTGAGCAGTTACAGCAGGAAGTGC
TACTGGGTAGCGCACCTAAGGGCGTGGCGCTTGTCAGCGGTGAGGATATGCAGTTGTCTGCCAGCGATAATCTGACTCTG
ACAGCAGGTAAGCAACTGGATATTGGCGTACAGAAAGACTTTACGCTGGCAGCAGGAAAACAGCTCAGCTTGTATAGCCG
TGCGGGAGCAAAGCTCTTTAGCTCACATAACGATATTGATATTCAGGCGCAGGGGGGAAATCTGACCACCTGGTCAACAC
AGGACACCTATATATCGAGTGGTAAAAAGCTGGTGGTGACAGCGCAGGATGAGTTAACACTGATTTGTGGCGGGGGCTAT
ATCAAAATTAAAAGTGGCAATGTGGAGATTGGCGGTCCGGGAAAACTATTGGTTAAAAATGCCGGTATTAAGAAAGCAGG
CTCCGGTAGTATGCAGGGCGTGATGAAGTCGTTTGAGCCTGAAAGTTTCGATGAAAATTTCATTATTTCGAATCCAGCAT
CAGGTAAGCCGATAGCAAATCAAAAATATGAAATACACATGCCAGATGGGAGTGTTATTGCCGGGATAACCGATGCAATG
GGGTATTCCTCTCTTGTTGCGTCAAAAGCATCTGATGACCTAAAAATTATTCTGAAAAAATGA