Detailed information of component protein


Summary

Component ID T6CP000296
Component protein type TssH
T6SS ID (Type) T6SS00037 experimental (Type i2)
Strain Escherichia coli CFT073
Replicon chromosome
Sequence Protein sequence (879 a.a.); Nucleotide sequence (2637 bp)
Reference
experimental Experimental investigation has been performed on this T6SS
External database links

Locus tag (Gene) C_RS16095
Coordinate (Strand) 3229545..3232181 (+)
NCBI ID WP_000147339.1
RefSeq NC_004431
Uniprot ID A0A0H2VA60_ECOL6, A0A234PTB2_ECOLX
KEGG ID ecc:c3392
PDB ID -
Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
AAA_2 PF07724.16 3.6e-38 609..774
AAA_lid_9 PF17871.3 1.5e-29 380..471
ClpB_D2-small PF10431.11 6.6e-14 780..855
AAA PF00004.31 1.1e-09 240..352
Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-878 MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLKLLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPRPVRERPQLSEPLAALIRQAWLIASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPARQPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVFGRDTEIRQMVDILSRRRKNNPILVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTIIGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKARYAQHHGVHMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLTQLHVRLAALDIEREAIEQDSVFYPEASPERLAELTDLRDELQAEAGHLEAQYQQEKALAQQIMTLRQEGTDSTELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPLSSLLKDEQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKTETALTLADTLFGGEKSLITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRPYSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREIDFRNTVILMTANLGSDHIMQLLEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVARRLHQHYGLETEISNSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHHKPARLRLDWDDEDGIVLEFDEK


Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
Other 0.7402 - -


  Protein sequence: 879 a.a.    

>T6CP000296 NC_004431:3229545-3232181 [Escherichia coli CFT073] [TssH]
MTGNHPAALLRRLNPYCARALDAAASLCQTRAHAEITIEHWLLKLLEQGEGDITVIARRYEWDIDTLWQSLLAHLDTLPR
PVRERPQLSEPLAALIRQAWLIASLEGDDPQIRSQHLLMALTEKPMLPACNDLWVLLSLSRVQLERLRPLLDAQSDECPA
RQPQVTEPLTSALPETATADAPAKTLTEKQDDALLAVLNRFTEDVTEKARSGRIDPVFGRDTEIRQMVDILSRRRKNNPI
LVGEPGVGKTALVEGLALRITEGNVPDSLKTVHIRTLDLGLLQAGAGVKGEFEQRLKNVIDAVQKSPEPVLLFIDEAHTI
IGAGNQAGGADAANLLKPALARGELRTIAATTWSEYKQYFERDAALERRFQMVKVDEPDDDTACLMLRGLKARYAQHHGV
HMLDSAIQTAVRLSRRYLTGRQLPDKAVDLLDTAGARVRMSLDTLPEPLTQLHVRLAALDIEREAIEQDSVFYPEASPER
LAELTDLRDELQAEAGHLEAQYQQEKALAQQIMTLRQEGTDSTELQQQLRTHQGFAPLLALDVDARAVATVVADWTGIPL
SSLLKDEQSDLLSMEQSLENRVVGQSPALCAIAQRLRAAKTGLTPENGPQGVFLLTGPSGTGKTETALTLADTLFGGEKS
LITINLSEYQEPHTVSQLKGSPPGYVGYGQGGVLTEAVRKRPYSVVLLDEVEKAHRDVMNLFYQVFDRGFMRDGEGREID
FRNTVILMTANLGSDHIMQLLEEKPDATDADLHELLYPLLREHFQPALMARFQTVIYRPLGQEAMRAIVEMKLAQVARRL
HQHYGLETEISNSLYDALTAACLLPDTGARNIDSLLNQQILPVLSQQLLAQQAVHHKPARLRLDWDDEDGIVLEFDEK*

  Nucleotide sequence: 2637 bp    

>T6CP000296 NC_004431:3229545-3232181 [Escherichia coli CFT073] [TssH]
ATGACAGGAAATCACCCCGCCGCGCTGCTGCGTCGCCTTAACCCATACTGTGCACGGGCGCTGGACGCTGCCGCCTCACT
GTGTCAGACCCGCGCCCATGCGGAAATAACCATTGAACACTGGCTGCTGAAACTGCTGGAGCAGGGAGAAGGCGATATCA
CGGTGATTGCCCGCCGCTATGAATGGGATATCGACACGCTCTGGCAGTCTCTGCTGGCACATCTGGACACCTTACCCCGC
CCGGTCCGCGAACGTCCTCAACTTTCTGAACCGCTGGCAGCGCTTATCCGACAGGCGTGGCTGATAGCGTCACTGGAAGG
CGACGATCCACAAATCCGCAGCCAGCATCTGCTGATGGCGCTGACAGAAAAACCGATGCTGCCCGCCTGTAATGACCTGT
GGGTATTGCTGAGTCTGAGCCGCGTGCAGCTTGAGCGGCTGCGTCCCCTGCTGGATGCGCAGTCGGATGAATGTCCGGCA
CGTCAGCCACAGGTCACCGAACCGCTGACCTCTGCACTGCCGGAGACGGCAACGGCGGACGCACCGGCAAAAACGCTGAC
GGAGAAACAGGATGACGCCCTGCTGGCGGTGCTTAACCGCTTTACCGAAGACGTGACGGAAAAAGCCCGCAGCGGGCGAA
TCGACCCGGTATTCGGGCGCGACACGGAAATTCGCCAGATGGTCGATATCCTCTCCCGTCGCCGCAAAAACAACCCGATT
CTGGTGGGAGAACCGGGGGTGGGCAAAACCGCGCTGGTGGAAGGGCTGGCGCTGCGTATCACCGAAGGCAACGTGCCGGA
CAGCCTGAAAACGGTGCATATCCGCACACTGGACCTCGGTCTGTTACAGGCTGGCGCGGGCGTTAAAGGTGAATTTGAAC
AGCGGCTGAAAAATGTCATCGATGCAGTGCAGAAATCACCGGAGCCGGTACTGCTGTTTATTGATGAAGCCCATACCATT
ATCGGTGCGGGTAATCAGGCAGGCGGCGCGGATGCGGCGAACCTGCTGAAACCGGCACTGGCAAGGGGCGAACTGCGCAC
CATCGCGGCGACCACGTGGAGCGAATACAAACAGTATTTTGAGCGCGACGCCGCGCTGGAGCGCCGCTTCCAGATGGTTA
AGGTTGACGAGCCGGATGATGACACCGCCTGCCTGATGCTCAGGGGGCTGAAAGCCCGCTATGCGCAGCACCACGGCGTA
CATATGCTGGACAGCGCCATTCAGACCGCCGTGCGCCTGTCGCGCCGCTATCTGACCGGACGCCAGTTGCCGGACAAGGC
GGTTGATTTGCTGGATACCGCCGGGGCAAGAGTCCGCATGAGCCTTGACACCCTGCCGGAACCGTTGACGCAGCTTCATG
TGCGACTGGCGGCACTGGATATTGAGCGGGAAGCGATTGAGCAGGACAGCGTATTTTATCCCGAAGCCAGCCCGGAGCGG
CTGGCGGAACTGACCGATTTGCGTGATGAGCTACAGGCAGAAGCCGGGCATCTGGAAGCGCAGTATCAGCAGGAAAAGGC
ACTGGCGCAGCAGATTATGACGTTGCGTCAGGAAGGGACAGACAGCACTGAACTGCAACAGCAACTGCGAACGCATCAGG
GCTTTGCACCGCTGCTGGCGCTGGATGTGGACGCCCGCGCCGTCGCCACGGTGGTGGCGGACTGGACCGGCATCCCGCTA
TCTTCCCTGCTCAAGGACGAGCAGAGCGACCTGCTCAGTATGGAACAGAGTCTTGAAAACCGCGTTGTCGGGCAAAGCCC
GGCGCTCTGCGCCATCGCACAGCGGCTGCGGGCGGCTAAGACCGGCCTCACGCCGGAGAACGGCCCGCAGGGGGTATTCC
TGCTGACCGGCCCCAGCGGCACCGGTAAAACCGAAACTGCGCTCACACTGGCCGACACTCTGTTTGGCGGTGAAAAATCC
CTTATCACCATCAACCTCTCCGAATATCAGGAGCCGCATACCGTCTCCCAGCTTAAGGGGTCTCCTCCGGGCTACGTTGG
CTACGGTCAGGGTGGCGTACTCACCGAAGCCGTTCGCAAACGCCCTTACAGCGTGGTGCTGCTCGACGAAGTGGAAAAGG
CGCACCGCGACGTAATGAACCTGTTCTATCAGGTATTTGACCGGGGCTTTATGCGCGACGGCGAAGGCCGGGAAATTGAC
TTCCGCAACACCGTGATTCTGATGACGGCTAATCTGGGCAGCGACCACATCATGCAACTGCTGGAGGAAAAACCGGACGC
CACGGACGCAGACCTGCATGAACTGCTGTATCCCCTGCTGCGCGAGCACTTCCAGCCAGCACTGATGGCGCGTTTTCAGA
CGGTGATTTACCGCCCGCTGGGACAGGAGGCGATGCGCGCCATTGTGGAAATGAAACTGGCGCAGGTGGCCCGCCGTCTT
CACCAGCACTATGGGCTGGAAACGGAAATCAGTAACAGCCTGTACGACGCCCTGACCGCCGCCTGCCTGCTGCCGGACAC
CGGTGCGCGTAATATCGACAGCCTGCTGAACCAGCAAATCCTGCCGGTCTTAAGCCAGCAGTTGCTGGCGCAGCAGGCCG
TGCATCATAAGCCTGCCCGACTGCGGCTTGACTGGGATGATGAAGACGGGATTGTGCTGGAATTTGATGAGAAATAA