SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000295
Component protein type	TssL
T6SS ID (Type)	T6SS00037 (Type i2)
Strain	Escherichia coli CFT073
Replicon	chromosome
Sequence	Protein sequence (222 a.a.); Nucleotide sequence (666 bp)
Reference	[1] Lloyd AL, Henderson TA, Vigil PD, et al. Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 2009 Jun;191(11):3469-81. doi: 10.1128/JB.01717-08. Epub 2009 Mar 27. PMID: 19329634 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Leiman PG, Basler M, Ramagopal UA, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4154-9. doi: 10.1073/pnas.0813360106. Epub 2009 Feb 27. PMID: 19251641

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C_RS16080
Coordinate (Strand)	3226474..3227139 (+)
NCBI ID	WP_000712315.1
RefSeq	NC_004431
Uniprot ID	A0A0H2VBP5_ECOL6, A0A2G9AAX9_ECOLX
KEGG ID	ecc:c3388
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.1e-44	9..207

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-181	MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSGMDDGQTIWMKDPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVAPFGVLPETAVLNVPLSTRQHP
Transmembrane helix	182-204	LLHSPFFWLVTLALLLAGVWWGL
Outside	205-221	HHWLNVLVDELLPQSLR

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9781	-	-

Protein sequence: 222 a.a.

						>T6CP000295 NC_004431:3226474-3227139 [Escherichia coli CFT073] [TssL]
MKKETDINIDALLRDTFLTVVELRQGTTVRHGMELYRHCQRQVELVRERLKDAGFSRENVEHITYAQCALLDETVLSRSG
MDDGQTIWMKDPLQSHFFNTLQAGELLYERMKQVLQEPAPAQAVLTCFHRVLLLGFRGRYQDPAAPERDQLISTLNGQVA
PFGVLPETAVLNVPLSTRQHPLLHSPFFWLVTLALLLAGVWWGLHHWLNVLVDELLPQSLR*					

Nucleotide sequence: 666 bp

						>T6CP000295 NC_004431:3226474-3227139 [Escherichia coli CFT073] [TssL]
ATGAAAAAAGAGACGGATATCAATATCGACGCGCTGCTGCGCGACACGTTTCTGACGGTGGTGGAACTGCGTCAGGGAAC
CACCGTGCGTCACGGTATGGAGCTGTATCGCCACTGCCAGCGGCAGGTGGAACTGGTCCGCGAACGTCTGAAAGATGCCG
GATTCAGTCGGGAGAACGTGGAGCACATAACTTACGCGCAGTGCGCCCTGCTGGACGAAACGGTCCTGAGCCGTAGCGGC
ATGGACGACGGTCAGACCATTTGGATGAAGGATCCACTCCAGTCGCATTTCTTCAACACCCTGCAGGCCGGTGAGCTGCT
GTACGAGCGGATGAAGCAGGTGCTACAGGAACCCGCCCCCGCGCAGGCCGTGCTGACCTGCTTTCACCGTGTCCTGCTGC
TGGGATTTCGCGGGCGTTATCAGGATCCGGCAGCCCCGGAGCGCGACCAGCTTATTTCGACGCTCAACGGGCAGGTCGCG
CCATTTGGTGTGCTGCCGGAAACGGCGGTACTGAACGTGCCGTTATCCACCCGTCAGCACCCTTTGCTGCATTCGCCATT
TTTCTGGCTGGTAACACTGGCGCTGCTACTGGCGGGCGTCTGGTGGGGGCTACACCACTGGCTGAATGTACTGGTGGATG
AACTGCTGCCGCAAAGCCTGCGTTAA					