SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000294
Component protein type	TssK
T6SS ID (Type)	T6SS00037 (Type i2)
Strain	Escherichia coli CFT073
Replicon	chromosome
Sequence	Protein sequence (446 a.a.); Nucleotide sequence (1338 bp)
Reference	[1] Lloyd AL, Henderson TA, Vigil PD, et al. Genomic islands of uropathogenic Escherichia coli contribute to virulence. J Bacteriol. 2009 Jun;191(11):3469-81. doi: 10.1128/JB.01717-08. Epub 2009 Mar 27. PMID: 19329634 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Leiman PG, Basler M, Ramagopal UA, et al. Type VI secretion apparatus and phage tail-associated protein complexes share a common evolutionary origin. Proc Natl Acad Sci U S A. 2009 Mar 17;106(11):4154-9. doi: 10.1073/pnas.0813360106. Epub 2009 Feb 27. PMID: 19251641

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	C_RS16075
Coordinate (Strand)	3225140..3226477 (+)
NCBI ID	WP_000686689.1
RefSeq	NC_004431
Uniprot ID	A0A0H2VA58_ECOL6, X5FV39_ECOLX
KEGG ID	ecc:c3387
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_VasE	PF05936.14	5.9e-131	18..442

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-445	MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGIANVLFDRDALALGKLKTQAVRLRFADGTLIDSDVSDVLPLACDLRALKNDSAIVLLALPLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQDYITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMRRESNQRMADFAVADVSLFWLLNALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLTFSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIALDLASLPGNRWKADLHDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTSVPAALPLRLENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9689	-	-

Protein sequence: 446 a.a.

						>T6CP000294 NC_004431:3225140-3226477 [Escherichia coli CFT073] [TssK]
MKICRPLWAEGTFLSSQQFQQQARWEAFSNDSIAQLCIRHPWGIANVLFDRDALALGKLKTQAVRLRFADGTLIDSDVSD
VLPLACDLRALKNDSAIVLLALPLAHGNGGNLGQGEQTERPLRYRQEWQKVQDIYGSDSEDMAVERHALSLRFAHDNNQD
YITCPLARLVRDVQGNWTQDESYIPPLLAFNAHDGLVQRLDTLLLQLRAKCQRLMAMRRESNQRMADFAVADVSLFWLLN
ALNSAEPVLSDFLRYPAVHPELVWRELARLAGALLTFSLEHNVSAVPPYVHESPSIVFPPLFSLLSELLEASLPSRVIAL
DLASLPGNRWKADLHDPRLREEADFYLSVRSSLPAHQVLHQLPLVCKIGAPDDVTLLINVALNGVPLVPLTSVPAALPLR
LENQYFALDMHSDAAKSMLESGCCMIYAPGTMGDLKPELFAVLRT*					

Nucleotide sequence: 1338 bp

						>T6CP000294 NC_004431:3225140-3226477 [Escherichia coli CFT073] [TssK]
ATGAAAATTTGTCGACCACTGTGGGCTGAGGGGACATTCCTGTCTTCTCAGCAGTTTCAGCAGCAGGCCCGCTGGGAAGC
GTTCAGCAATGACAGCATTGCGCAGCTTTGTATTCGCCATCCGTGGGGGATCGCAAACGTGCTCTTTGACCGGGATGCCC
TGGCGCTCGGAAAGCTGAAAACGCAGGCAGTACGCCTGCGTTTTGCTGATGGTACGCTGATCGATTCAGACGTGAGTGAC
GTTCTGCCGCTCGCCTGCGATCTGCGCGCGCTGAAGAACGACAGCGCGATTGTCCTGCTGGCGCTGCCGCTTGCACATGG
TAACGGCGGTAATCTCGGTCAGGGTGAGCAGACTGAAAGACCGCTGCGCTATCGTCAGGAATGGCAAAAGGTGCAGGATA
TCTACGGCAGTGACAGTGAAGATATGGCCGTTGAGCGTCATGCGCTGTCCCTGCGCTTTGCTCATGATAACAATCAGGAT
TACATCACCTGCCCACTGGCGCGTCTGGTGCGGGATGTTCAGGGCAACTGGACACAGGATGAGAGCTATATTCCGCCGTT
ACTGGCGTTTAACGCCCATGACGGGCTGGTACAGCGTCTGGACACGCTGCTGTTGCAGCTACGGGCCAAATGTCAGCGCC
TGATGGCGATGCGCCGGGAAAGCAACCAGCGTATGGCGGACTTCGCCGTCGCCGATGTCTCCCTGTTCTGGCTGCTTAAC
GCGCTGAACTCCGCCGAGCCGGTACTGAGTGATTTCCTGCGCTACCCGGCGGTTCATCCCGAACTGGTCTGGCGGGAGCT
GGCGAGGCTTGCCGGTGCGCTGCTGACGTTTTCCCTTGAACATAACGTCAGCGCCGTTCCGCCCTATGTCCACGAATCGC
CGTCCATCGTCTTTCCGCCACTTTTCTCCCTGCTCAGCGAACTGCTGGAAGCCAGCCTGCCGTCACGGGTGATCGCGCTG
GATCTGGCGTCCCTGCCGGGCAACCGCTGGAAAGCAGACCTGCACGATCCGCGCCTGCGGGAAGAGGCCGACTTTTACCT
GTCCGTTCGTTCCTCTCTGCCTGCGCATCAGGTACTGCACCAGCTACCGCTGGTCTGCAAAATCGGTGCGCCTGATGACG
TCACGCTGCTTATCAATGTGGCGCTGAATGGTGTTCCACTGGTGCCGCTTACCTCAGTTCCGGCAGCGCTGCCACTGCGG
CTGGAGAACCAGTATTTTGCGCTGGATATGCACAGTGACGCGGCTAAATCGATGCTGGAGTCCGGGTGCTGCATGATTTA
TGCGCCGGGAACAATGGGCGACCTGAAACCTGAACTGTTTGCGGTGCTGCGCACATGA					