SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000166
Component protein type	TssJ
T6SS ID (Type)	T6SS00022 (Type Unknown)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (182 a.a.); Nucleotide sequence (546 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS18950
Coordinate (Strand)	4004212..4004757 (+)
NCBI ID	WP_002212104.1
RefSeq	NC_003143
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3.8e-30	52..172

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-6	MNNARG
Transmembrane helix	7-29	YLLTWALCVVFLLSGCSGAWEAT
Outside	30-181	KKVGKVVMDPDIPVGQATDQPSTVRLTLLSDPDTNPNEKGEAVPTEIVVVYLSENSKLLAADYDQLSSDALEKTLGKNYIDHQEYTLLPDQFKALDPIKLEEKNSYLGVIAHYADANRSEWKKIIKIKGIGHNYQVLVHVRSNDVDLRKEEE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9974	pos: 21-22. LSG-CS.	MNNARGYLLTWALCVVFLLSG

Protein sequence: 182 a.a.

						>T6CP000166 NC_003143:4004212-4004757 [Yersinia pestis CO92] [TssJ]
MNNARGYLLTWALCVVFLLSGCSGAWEATKKVGKVVMDPDIPVGQATDQPSTVRLTLLSDPDTNPNEKGEAVPTEIVVVY
LSENSKLLAADYDQLSSDALEKTLGKNYIDHQEYTLLPDQFKALDPIKLEEKNSYLGVIAHYADANRSEWKKIIKIKGIG
HNYQVLVHVRSNDVDLRKEEE*					

Nucleotide sequence: 546 bp

						>T6CP000166 NC_003143:4004212-4004757 [Yersinia pestis CO92] [TssJ]
ATGAACAACGCCAGAGGCTATCTCCTGACGTGGGCATTATGTGTGGTGTTCCTGTTGTCTGGGTGCAGCGGTGCATGGGA
AGCCACTAAAAAGGTCGGCAAGGTGGTTATGGACCCTGATATTCCGGTGGGGCAGGCTACCGATCAACCCTCAACGGTCA
GGCTAACATTGCTGTCCGATCCCGATACCAACCCTAATGAAAAAGGGGAAGCGGTACCGACTGAGATAGTGGTGGTGTAC
CTAAGTGAAAACTCCAAGCTGCTGGCCGCGGATTATGATCAGCTAAGCAGTGACGCGTTGGAGAAAACGCTGGGTAAGAA
TTACATCGATCATCAGGAATACACCTTACTGCCCGATCAATTCAAGGCGCTGGACCCTATCAAGCTGGAAGAGAAAAACA
GCTATCTGGGGGTGATTGCTCACTATGCCGATGCCAACCGCAGTGAATGGAAGAAGATCATCAAAATAAAAGGGATCGGC
CATAACTACCAGGTTTTGGTCCATGTCAGATCAAACGATGTCGATCTAAGGAAAGAGGAAGAATAA					