SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000164
Component protein type	TssA
T6SS ID (Type)	T6SS00021 (Type i3)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (394 a.a.); Nucleotide sequence (1182 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS15705
Coordinate (Strand)	3295607..3296788 (+)
NCBI ID	WP_002211584.1
RefSeq	NC_003143
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
ImpA_N	PF06812.14	1.3e-29	17..140

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-393	MSEQSIAERAGYYQSMMQPIPGDFPCGKNLEYDPAFIMLQTKLQPKQAAEYGNFVEVAEPNNWTDIERKCLELLNNSRDIRLFIILMRCRMRQVGVGALQEGLEAILWCLDTWPDQLHPQLFDEGEFEPLMRGNAFAELEDIDGFIADFRNQQLPKAAGVQLSIKEFEKAHSVPREEGALDEGTLEAVKQEWQNRSDHTILSLQHAYALLQGLATRLNNSLGEDTPDFVRMTSVLRHFDAAITPIAPIVVPIPEVVELYPVATAMLASTEMPVTAEVQTVAETQMAIPIEAPQQRQAVVTPSPQLQPLQLQSAQLQPVPIQSQPVPVQQQGIQNRADALARLIEVREWFTAMEPSSPVIALLAFSEKTIGKSFGELLQFIPQELISKLNEGQE

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9886	-	-

Protein sequence: 394 a.a.

						>T6CP000164 NC_003143:3295607-3296788 [Yersinia pestis CO92] [TssA]
MSEQSIAERAGYYQSMMQPIPGDFPCGKNLEYDPAFIMLQTKLQPKQAAEYGNFVEVAEPNNWTDIERKCLELLNNSRDI
RLFIILMRCRMRQVGVGALQEGLEAILWCLDTWPDQLHPQLFDEGEFEPLMRGNAFAELEDIDGFIADFRNQQLPKAAGV
QLSIKEFEKAHSVPREEGALDEGTLEAVKQEWQNRSDHTILSLQHAYALLQGLATRLNNSLGEDTPDFVRMTSVLRHFDA
AITPIAPIVVPIPEVVELYPVATAMLASTEMPVTAEVQTVAETQMAIPIEAPQQRQAVVTPSPQLQPLQLQSAQLQPVPI
QSQPVPVQQQGIQNRADALARLIEVREWFTAMEPSSPVIALLAFSEKTIGKSFGELLQFIPQELISKLNEGQE*					

Nucleotide sequence: 1182 bp

						>T6CP000164 NC_003143:3295607-3296788 [Yersinia pestis CO92] [TssA]
ATGAGTGAACAGAGTATCGCTGAACGTGCCGGTTATTATCAAAGCATGATGCAGCCCATTCCAGGTGACTTTCCTTGTGG
CAAAAATCTGGAATATGACCCGGCATTTATTATGCTACAGACAAAATTGCAACCTAAGCAGGCCGCTGAATACGGCAATT
TTGTTGAGGTCGCTGAACCCAATAACTGGACAGATATCGAGCGTAAATGCCTTGAACTCCTGAATAATTCTCGGGATATT
CGGCTGTTTATTATTCTGATGCGCTGCCGGATGCGTCAGGTCGGTGTGGGCGCGTTGCAAGAAGGGCTGGAAGCCATACT
TTGGTGCCTCGACACTTGGCCTGATCAGCTACATCCTCAGTTGTTTGATGAAGGGGAATTTGAACCTTTAATGCGCGGTA
ATGCTTTTGCTGAACTGGAAGATATTGATGGTTTTATCGCCGACTTCCGTAATCAGCAGTTACCCAAAGCCGCTGGCGTG
CAACTTTCCATCAAAGAGTTTGAAAAAGCACACTCCGTTCCTCGAGAAGAAGGGGCATTGGACGAAGGGACTCTGGAAGC
CGTTAAGCAAGAGTGGCAGAACCGATCAGATCACACCATTCTCTCACTCCAGCACGCTTATGCCTTGTTGCAAGGATTAG
CCACGCGCCTCAACAACTCCCTCGGTGAAGACACACCTGATTTTGTCAGAATGACCTCAGTGTTACGCCACTTTGATGCC
GCGATAACCCCGATAGCGCCTATCGTGGTGCCGATCCCAGAAGTTGTCGAGCTCTATCCAGTCGCCACAGCGATGCTAGC
AAGCACAGAAATGCCAGTGACCGCCGAGGTACAGACGGTTGCAGAGACACAAATGGCCATACCCATTGAAGCTCCCCAAC
AGCGTCAAGCGGTGGTAACCCCATCCCCACAGTTGCAGCCGTTACAGTTGCAATCGGCACAGTTGCAACCTGTACCGATA
CAGTCACAGCCGGTGCCTGTCCAGCAGCAAGGGATCCAAAACAGGGCTGATGCATTAGCCCGGCTTATAGAGGTGCGAGA
GTGGTTTACCGCCATGGAACCGAGTAGCCCGGTCATTGCCTTACTGGCTTTTTCAGAAAAAACTATCGGCAAAAGCTTCG
GCGAATTGCTGCAGTTCATTCCTCAGGAACTGATTTCGAAACTCAATGAAGGACAGGAGTAA					