Detailed information of component protein
Summary
| Component ID | T6CP000157 |
| Component protein type | TssJ |
| T6SS ID (Type) | T6SS00021  (Type i3) |
| Strain | Yersinia pestis CO92 |
| Replicon | chromosome |
| Sequence | Protein sequence (180 a.a.); Nucleotide sequence (540 bp) |
| Reference |
Bioinformatics investigation has been performed on this T6SSExternal database links
| Locus tag (Gene) | YPO_RS15640 |
| Coordinate (Strand) | 3278986..3279525 (-) |
| NCBI ID | WP_002211572.1 |
| RefSeq | NC_003143 |
| Uniprot ID | A0A0U1QVX5_YERP3, A0A3G5KK48_YERPU |
| KEGG ID | ypi:YpsIP31758_1370, ypc:BZ23_2244, ypq:DJ40_3880, ypu:BZ21_1960, ypr:BZ20_3546 |
| PDB ID | - |
Pfam domain hit(s)
| Domain | Pfam ID | E-value | Aligned region |
|---|---|---|---|
| T6SS-SciN | PF12790.9 | 1.3e-29 | 38..161 |
Transmembrane helices
- Transmembrane helices are predicted using TMHMM 2.0 software.
| Prediction | Region | Sequence |
|---|---|---|
| Inside | 1-6 | MNKSIR |
| Transmembrane helix | 7-26 | GVYRATQYLFLVLVTLLTIA |
| Outside | 27-179 | CSSKVQPEKAEKLTVEIATAADINPNDKGVANPLRIALYTLKSTDEFQSSDFFTITEENTPSLKEQMDNVFDRIMLPSETKKIELTLDAGVTAIGIVAAYREIEQAEWKAVIYPLPKKRVDPWYKKMWPGQDQQDPIMKVRVERLSISIKEMD |
Signal peptides
- Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
- Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
- Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
| Prediction | Probability | Cleavage site | Signal peptide sequence |
|---|---|---|---|
| LIPO(Sec/SPII) | 0.9977 | pos: 26-27. TIA-CS. | MNKSIRGVYRATQYLFLVLVTLLTIA |
Protein sequence: 180 a.a.
>T6CP000157 NC_003143:c3279525-3278986 [Yersinia pestis CO92] [TssJ]
MNKSIRGVYRATQYLFLVLVTLLTIACSSKVQPEKAEKLTVEIATAADINPNDKGVANPLRIALYTLKSTDEFQSSDFFT
ITEENTPSLKEQMDNVFDRIMLPSETKKIELTLDAGVTAIGIVAAYREIEQAEWKAVIYPLPKKRVDPWYKKMWPGQDQQ
DPIMKVRVERLSISIKEMD*
MNKSIRGVYRATQYLFLVLVTLLTIACSSKVQPEKAEKLTVEIATAADINPNDKGVANPLRIALYTLKSTDEFQSSDFFT
ITEENTPSLKEQMDNVFDRIMLPSETKKIELTLDAGVTAIGIVAAYREIEQAEWKAVIYPLPKKRVDPWYKKMWPGQDQQ
DPIMKVRVERLSISIKEMD*
Nucleotide sequence: 540 bp
>T6CP000157 NC_003143:c3279525-3278986 [Yersinia pestis CO92] [TssJ]
ATGAATAAAAGTATCCGAGGGGTTTACCGGGCGACACAGTACTTATTTTTGGTACTGGTAACGTTATTGACGATAGCCTG
TAGTTCAAAAGTGCAACCTGAAAAGGCTGAAAAGCTTACCGTCGAAATCGCGACAGCAGCGGATATTAATCCGAATGATA
AAGGGGTGGCGAACCCGCTAAGGATTGCACTTTATACACTCAAGAGCACCGATGAATTTCAGTCGAGTGATTTCTTCACT
ATCACTGAAGAAAATACGCCATCGCTAAAAGAACAAATGGATAACGTGTTTGACCGGATTATGTTGCCAAGCGAGACCAA
AAAAATAGAGCTGACACTTGATGCTGGCGTTACTGCTATCGGCATCGTGGCTGCTTATCGTGAAATTGAACAGGCGGAGT
GGAAAGCGGTTATTTATCCGTTGCCCAAAAAACGCGTGGATCCTTGGTATAAAAAAATGTGGCCAGGGCAAGACCAACAA
GATCCGATCATGAAGGTACGCGTTGAGCGTCTGTCAATATCAATTAAAGAAATGGATTAA
ATGAATAAAAGTATCCGAGGGGTTTACCGGGCGACACAGTACTTATTTTTGGTACTGGTAACGTTATTGACGATAGCCTG
TAGTTCAAAAGTGCAACCTGAAAAGGCTGAAAAGCTTACCGTCGAAATCGCGACAGCAGCGGATATTAATCCGAATGATA
AAGGGGTGGCGAACCCGCTAAGGATTGCACTTTATACACTCAAGAGCACCGATGAATTTCAGTCGAGTGATTTCTTCACT
ATCACTGAAGAAAATACGCCATCGCTAAAAGAACAAATGGATAACGTGTTTGACCGGATTATGTTGCCAAGCGAGACCAA
AAAAATAGAGCTGACACTTGATGCTGGCGTTACTGCTATCGGCATCGTGGCTGCTTATCGTGAAATTGAACAGGCGGAGT
GGAAAGCGGTTATTTATCCGTTGCCCAAAAAACGCGTGGATCCTTGGTATAAAAAAATGTGGCCAGGGCAAGACCAACAA
GATCCGATCATGAAGGTACGCGTTGAGCGTCTGTCAATATCAATTAAAGAAATGGATTAA