SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000150
Component protein type	TssJ
T6SS ID (Type)	T6SS00019 (Type i2)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (194 a.a.); Nucleotide sequence (582 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS08415
Coordinate (Strand)	1687503..1688084 (+)
NCBI ID	WP_002214552.1
RefSeq	NC_003143
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	6.7e-31	61..184

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-193	VLTTVWKNKTCTSKHKSWAAWKNKICIPALVLLLSGCGLTQAVTDSTVSATKSLFYKQIKVLHLDFTAREALNTDAHEANSSSEPVMVRVYQLRDDKTFLKTVYQQLASDGEAALKDDLLASRSVVVKPGGAVTLDMPMEKETQFVAVVALFRHPDMAKDHWRLILTLDDLHPDKPRTLELGNNSLTLRTEKK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9715	pos: 36-37. LSG-CG.	VLTTVWKNKTCTSKHKSWAAWKNKICIPALVLLLSG

Protein sequence: 194 a.a.

						>T6CP000150 NC_003143:1687503-1688084 [Yersinia pestis CO92] [TssJ]
VLTTVWKNKTCTSKHKSWAAWKNKICIPALVLLLSGCGLTQAVTDSTVSATKSLFYKQIKVLHLDFTAREALNTDAHEAN
SSSEPVMVRVYQLRDDKTFLKTVYQQLASDGEAALKDDLLASRSVVVKPGGAVTLDMPMEKETQFVAVVALFRHPDMAKD
HWRLILTLDDLHPDKPRTLELGNNSLTLRTEKK*					

Nucleotide sequence: 582 bp

						>T6CP000150 NC_003143:1687503-1688084 [Yersinia pestis CO92] [TssJ]
GTGTTAACTACCGTTTGGAAAAATAAAACCTGTACTTCGAAACATAAAAGCTGGGCCGCCTGGAAAAATAAAATCTGCAT
CCCCGCACTGGTCTTGTTGCTCAGTGGCTGCGGGCTGACGCAAGCCGTGACCGATAGCACCGTGTCGGCCACAAAATCAC
TGTTTTATAAGCAGATAAAGGTGCTGCATCTGGACTTTACCGCCCGTGAAGCCCTGAATACCGATGCGCACGAGGCGAAC
TCGTCATCGGAGCCGGTAATGGTGCGGGTTTATCAGTTACGTGATGATAAAACCTTCCTCAAAACGGTGTATCAACAACT
GGCAAGCGACGGGGAGGCGGCGCTTAAAGATGACCTGCTGGCCAGCCGCAGTGTGGTGGTGAAACCGGGCGGGGCGGTAA
CACTGGATATGCCGATGGAAAAAGAGACCCAGTTTGTGGCAGTGGTGGCGCTGTTCCGTCATCCGGATATGGCCAAAGAC
CACTGGCGTCTGATACTCACGCTGGACGACCTGCATCCCGATAAGCCCCGTACCCTTGAGTTGGGTAATAACAGCCTGAC
CCTGCGGACGGAGAAAAAATAA					