SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000148
Component protein type	TssF
T6SS ID (Type)	T6SS00019 (Type i2)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (588 a.a.); Nucleotide sequence (1764 bp)
Reference	[1] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603

Bioinformatics investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS08405
Coordinate (Strand)	1684716..1686479 (+)
NCBI ID	WP_002211940.1
RefSeq	NC_003143
Uniprot ID	-
KEGG ID	-
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	1.7e-184	1..584

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-587	MDDLTLRYFDAEMRYLREAGEEFARAHPDRAAGLNLDKAGARDPYVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLWPHYLRTIPSLSIVEFTPQWQGMKERMGVSKGFEVLSRPIGERETRCRYTTTQEMDLLPLTLRRAGLDSEPDGRSVIRLRFDCSALADWSRINLSRLPLYFDADAPLACALHEALTLNTAKLWIRLPGQADRQPLDGHFAPLGFGEQDTLWPKADSAFSGYQLLLEYFTFREKFMFVALQGLDGIELPAELPWFEIDVVLEKRWQHDFSFSEKNLRLHCVPVINLFPLESDPLSLSSLQTEYLLRPMRIQDGYTEVYSVDSVISSRHTGHQVYVPFTSFRHKGGMLRHDAPEYYYHTRVKRGPSGLHDTWLVLGGEAFDNHSVPDNENLSLSLTGTNGQLPRKALQSTVLDTAVKSTGAQVRVRNLSAPSLPCYPPNRDRFHWRVLSHLGSSFLWMMDNAEVLRGTLALYDWTDNEMNRRRLEAIAEVKHSEIERFERGYLLRGVHIEITLDSNGFTGTGDICLFGEMLSRFFALYTDIHLFNRLTLILQPTGERLEWEENHQSRLPG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9967	-	-

Protein sequence: 588 a.a.

						>T6CP000148 NC_003143:1684716-1686479 [Yersinia pestis CO92] [TssF]
MDDLTLRYFDAEMRYLREAGEEFARAHPDRAAGLNLDKAGARDPYVERLFEGFAFLMGRLREKLDDDLPELTEGLVSLLW
PHYLRTIPSLSIVEFTPQWQGMKERMGVSKGFEVLSRPIGERETRCRYTTTQEMDLLPLTLRRAGLDSEPDGRSVIRLRF
DCSALADWSRINLSRLPLYFDADAPLACALHEALTLNTAKLWIRLPGQADRQPLDGHFAPLGFGEQDTLWPKADSAFSGY
QLLLEYFTFREKFMFVALQGLDGIELPAELPWFEIDVVLEKRWQHDFSFSEKNLRLHCVPVINLFPLESDPLSLSSLQTE
YLLRPMRIQDGYTEVYSVDSVISSRHTGHQVYVPFTSFRHKGGMLRHDAPEYYYHTRVKRGPSGLHDTWLVLGGEAFDNH
SVPDNENLSLSLTGTNGQLPRKALQSTVLDTAVKSTGAQVRVRNLSAPSLPCYPPNRDRFHWRVLSHLGSSFLWMMDNAE
VLRGTLALYDWTDNEMNRRRLEAIAEVKHSEIERFERGYLLRGVHIEITLDSNGFTGTGDICLFGEMLSRFFALYTDIHL
FNRLTLILQPTGERLEWEENHQSRLPG*					

Nucleotide sequence: 1764 bp

						>T6CP000148 NC_003143:1684716-1686479 [Yersinia pestis CO92] [TssF]
ATGGACGACTTAACCCTGCGTTATTTTGACGCTGAAATGCGCTACCTGCGCGAAGCGGGCGAAGAGTTTGCTCGTGCGCA
CCCTGATCGGGCTGCCGGATTAAATCTGGATAAAGCCGGGGCGCGTGACCCCTATGTCGAACGCCTATTTGAAGGCTTTG
CCTTTCTGATGGGCCGACTGCGGGAAAAACTGGATGACGACCTGCCGGAACTGACCGAAGGGTTGGTCAGCCTGCTGTGG
CCGCACTACCTGCGTACCATTCCGTCGCTTTCTATTGTGGAGTTCACCCCACAATGGCAGGGGATGAAAGAGCGCATGGG
GGTGAGTAAAGGTTTTGAAGTGCTGTCCCGCCCCATTGGTGAACGTGAAACCCGTTGCCGCTATACCACCACGCAAGAGA
TGGACCTGCTCCCGCTGACCCTGCGCCGTGCCGGGCTGGACAGTGAACCCGATGGCCGGTCGGTTATCCGGCTGCGCTTT
GATTGCAGCGCGCTGGCCGACTGGAGCCGTATCAACCTGAGCCGCCTGCCGCTCTATTTTGATGCCGATGCGCCGCTGGC
CTGCGCCCTGCATGAGGCGCTGACCCTGAATACCGCCAAACTCTGGATCCGTTTGCCGGGGCAGGCTGATCGCCAGCCGC
TGGACGGCCATTTCGCGCCGCTGGGGTTCGGGGAGCAGGATACACTGTGGCCGAAGGCCGACAGTGCGTTCAGTGGTTAT
CAGCTCTTGCTGGAGTACTTTACCTTCCGCGAAAAGTTTATGTTTGTCGCGTTGCAGGGGCTGGATGGGATCGAGCTGCC
GGCGGAACTGCCGTGGTTTGAGATTGATGTGGTGCTGGAAAAACGCTGGCAACATGATTTCTCGTTCTCCGAAAAGAACC
TGCGCCTGCATTGTGTGCCGGTGATTAACCTGTTCCCGCTGGAATCGGACCCGCTGTCACTTTCTTCATTACAGACCGAA
TACCTGCTGCGCCCAATGCGCATTCAGGACGGTTATACCGAAGTGTATTCGGTGGACTCGGTGATCTCCTCCCGTCATAC
CGGGCATCAGGTCTATGTCCCTTTCACCAGCTTTCGCCACAAGGGGGGGATGTTGCGCCATGATGCGCCGGAGTATTACT
ACCATACGCGGGTCAAACGCGGGCCTTCGGGGTTACACGATACCTGGCTGGTGCTGGGGGGCGAGGCGTTTGATAACCAC
AGCGTACCGGATAATGAAAATTTGTCGCTGAGCCTGACCGGCACCAACGGGCAGTTACCGCGTAAAGCGCTGCAAAGCAC
GGTGCTGGATACGGCGGTGAAATCCACCGGGGCTCAGGTGCGGGTGCGTAATCTCTCCGCGCCGTCATTGCCATGCTACC
CGCCGAACCGCGACCGTTTTCACTGGCGGGTGCTGAGCCATCTGGGCAGTAGTTTTCTGTGGATGATGGATAACGCCGAA
GTGCTGCGCGGCACGCTGGCGCTGTATGACTGGACGGACAACGAAATGAACCGCCGTCGGCTGGAGGCGATCGCCGAGGT
CAAACACAGCGAGATTGAACGCTTTGAGCGGGGCTATTTGCTGCGCGGGGTGCACATTGAAATCACCCTCGACAGCAACG
GCTTTACCGGTACCGGGGATATTTGTCTGTTTGGGGAAATGCTCAGTCGCTTCTTTGCCCTGTATACCGATATTCACCTG
TTTAATCGTCTGACTCTGATACTGCAACCGACAGGAGAACGTTTGGAATGGGAAGAGAATCATCAGTCCCGCCTTCCCGG
CTAA					