SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000136
Component protein type	TssL
T6SS ID (Type)	T6SS00017 (Type i3)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (537 a.a.); Nucleotide sequence (1611 bp)
Reference	[1] Robinson JB, Telepnev MV, Zudina IV, et al. Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models. Microb Pathog. 2009 Nov;47(5):243-51. doi: 10.1016/j.micpath.2009.08.005. Epub 2009 Aug 27. PMID: 19716410 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Cathelyn JS, Crosby SD, Lathem WW, et al. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. Epub 2006 Aug 28. PMID: 16938880

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS03580
Coordinate (Strand)	551203..552813 (+)
NCBI ID	WP_002210469.1
RefSeq	NC_003143
Uniprot ID	A0A0E1NRM6_YERPA, A0A0H2YFI1_YERPN, A0A454Y5W9_YERPE
KEGG ID	ype:YPO0514, ypn:YPN_0389, ypa:YPA_3581
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
DotU	PF09850.11	1.6e-42	145..336
OmpA	PF00691.22	8e-10	398..501

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Inside	1-315	MNEFERQIRAAISAARNGAKHAEQSLTTPMWQAKSTVASLGGIVPRSGSSSTSQAENYKEGLADQAASGNNMARTSAPPVTLYQQQPNANDSYPNGNNNNPNGDNNNPNGSNNNIARVQRMPHGISRGLYERPGMLLGAWDNAYIAAAMPLLLLVENIRSWPTRNAAEVRPPIVRELQYFQQHLQKKNYPQEDINHLSYLLCTYIDGIFNGLQTPDSYNQSLLVEFHRDAWGGEDCFEHLRVYMNSPKQYREVLEFYDLIMCLGFDGKYQMIEHGAVLLMDLRSRLHTQLYGQDATQSLAIAQAVKGSPRRQYIK
Transmembrane helix	316-338	ALKIFTYGFALCLCAYGVTAWYL
Outside	339-536	HQQSQQIRSNILTWVLPEPRKINIMETLPNPLSNILNEGWLEVRKDPRGWLLIFTSDGAFRTGEATLSEEFINKKNIERLGLALAPWPGDIEVIGHTDNKPFRSTSGNNNLKLSAARASVVADKLRESTQINETHQREISAIGRGESDPLADNATEEGRKRNRRVDILWKIGQRDADKAMKQFLENPTPEVQGTNTQQ

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.6782	-	-

Protein sequence: 537 a.a.

						>T6CP000136 NC_003143:551203-552813 [Yersinia pestis CO92] [TssL]
MNEFERQIRAAISAARNGAKHAEQSLTTPMWQAKSTVASLGGIVPRSGSSSTSQAENYKEGLADQAASGNNMARTSAPPV
TLYQQQPNANDSYPNGNNNNPNGDNNNPNGSNNNIARVQRMPHGISRGLYERPGMLLGAWDNAYIAAAMPLLLLVENIRS
WPTRNAAEVRPPIVRELQYFQQHLQKKNYPQEDINHLSYLLCTYIDGIFNGLQTPDSYNQSLLVEFHRDAWGGEDCFEHL
RVYMNSPKQYREVLEFYDLIMCLGFDGKYQMIEHGAVLLMDLRSRLHTQLYGQDATQSLAIAQAVKGSPRRQYIKALKIF
TYGFALCLCAYGVTAWYLHQQSQQIRSNILTWVLPEPRKINIMETLPNPLSNILNEGWLEVRKDPRGWLLIFTSDGAFRT
GEATLSEEFINKKNIERLGLALAPWPGDIEVIGHTDNKPFRSTSGNNNLKLSAARASVVADKLRESTQINETHQREISAI
GRGESDPLADNATEEGRKRNRRVDILWKIGQRDADKAMKQFLENPTPEVQGTNTQQ*					

Nucleotide sequence: 1611 bp

						>T6CP000136 NC_003143:551203-552813 [Yersinia pestis CO92] [TssL]
ATGAACGAATTTGAACGCCAGATCCGTGCAGCCATTTCCGCAGCACGCAATGGCGCAAAACATGCGGAACAGTCACTGAC
TACACCAATGTGGCAAGCCAAAAGCACCGTAGCCTCATTGGGTGGGATTGTCCCTAGAAGTGGCTCTTCGTCAACGTCAC
AGGCGGAGAACTATAAGGAAGGTCTCGCGGACCAGGCTGCCTCGGGCAACAACATGGCGCGCACGAGTGCGCCACCGGTC
ACTTTGTATCAGCAACAGCCAAATGCGAATGACAGCTATCCAAACGGGAATAACAACAATCCAAACGGGGATAACAACAA
TCCAAACGGGAGTAACAACAATATAGCGAGAGTACAGCGTATGCCGCATGGCATTTCCAGGGGCTTATATGAGCGCCCTG
GGATGTTATTGGGTGCCTGGGATAACGCCTATATTGCTGCGGCTATGCCTTTGCTGCTGCTGGTGGAAAATATTCGTAGC
TGGCCGACGCGTAACGCCGCAGAGGTCAGGCCACCGATTGTGCGGGAATTACAATATTTCCAGCAACATTTGCAGAAAAA
GAACTACCCGCAAGAAGACATTAACCACCTGTCTTACCTGCTATGTACCTATATCGATGGCATTTTTAACGGGCTGCAAA
CCCCAGACTCCTACAACCAAAGTCTGTTAGTGGAGTTTCACCGTGATGCCTGGGGGGGTGAGGACTGCTTCGAACATCTG
CGGGTCTATATGAACTCGCCGAAACAGTACCGGGAAGTTCTGGAATTCTATGATCTGATTATGTGCCTTGGTTTTGACGG
TAAATACCAGATGATAGAGCATGGTGCGGTTCTGCTGATGGATTTACGCAGCCGTCTCCACACGCAACTCTACGGTCAGG
ACGCCACACAATCTTTGGCTATCGCGCAAGCGGTCAAAGGTTCTCCGCGTCGCCAATATATCAAGGCGCTGAAAATCTTC
ACCTATGGTTTCGCACTGTGCCTTTGTGCTTACGGCGTCACGGCGTGGTATCTGCACCAGCAATCCCAACAGATCCGCAG
CAACATTCTGACGTGGGTACTGCCTGAACCGCGGAAAATCAACATCATGGAGACCTTGCCGAATCCGCTATCCAACATCC
TGAATGAAGGGTGGCTGGAGGTCAGGAAAGATCCGCGTGGATGGCTATTAATCTTCACCTCCGACGGCGCGTTCCGCACG
GGTGAAGCGACCCTCTCGGAAGAGTTTATCAACAAGAAGAATATCGAACGTCTTGGGCTGGCATTAGCCCCATGGCCGGG
AGATATCGAGGTTATTGGTCATACGGATAACAAACCGTTCCGTAGCACTTCCGGTAACAACAACCTCAAACTTTCCGCGG
CCAGAGCATCGGTGGTGGCAGATAAACTGCGGGAATCCACTCAAATCAACGAAACCCATCAGCGAGAAATAAGTGCCATC
GGACGGGGGGAGAGCGATCCTTTAGCTGACAATGCAACGGAAGAAGGGCGCAAGCGTAACCGGCGTGTGGATATCCTATG
GAAAATTGGTCAGCGCGATGCCGATAAGGCCATGAAGCAATTCCTGGAGAACCCAACACCAGAAGTTCAAGGAACGAATA
CCCAACAATAG					