SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000134
Component protein type	TssJ
T6SS ID (Type)	T6SS00017 (Type i3)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (164 a.a.); Nucleotide sequence (492 bp)
Reference	[1] Robinson JB, Telepnev MV, Zudina IV, et al. Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models. Microb Pathog. 2009 Nov;47(5):243-51. doi: 10.1016/j.micpath.2009.08.005. Epub 2009 Aug 27. PMID: 19716410 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Cathelyn JS, Crosby SD, Lathem WW, et al. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. Epub 2006 Aug 28. PMID: 16938880

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS03570
Coordinate (Strand)	549240..549731 (+)
NCBI ID	WP_002210471.1
RefSeq	NC_003143
Uniprot ID	A0A0E1NRQ4_YERPA, A0A0H2YFT4_YERPN, A0A2U2H095_YERPE
KEGG ID	ype:YPO0512, ypa:YPA_3583, ypn:YPN_0387
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS-SciN	PF12790.9	3e-18	37..152

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-4	MRSL
Transmembrane helix	5-27	FRVLPIFILTGCVLLSGCSWFSS
Inside	28-163	SSEAALQKRRIDLQVVAAPLINPGPNGQAQPLKVCIIELNKEGWSPPGLYQGTLCSGISVGGEVVSVTEYILAPTEVRQYSRDVPFEQERWWAIAAEFQEMSNGKSLLTLKSDARADFNRVVLVDGKALSLKASTK

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
LIPO(Sec/SPII)	0.9985	pos: 21-22. LSG-CS.	MRSLFRVLPIFILTGCVLLSG

Protein sequence: 164 a.a.

						>T6CP000134 NC_003143:549240-549731 [Yersinia pestis CO92] [TssJ]
MRSLFRVLPIFILTGCVLLSGCSWFSSSSEAALQKRRIDLQVVAAPLINPGPNGQAQPLKVCIIELNKEGWSPPGLYQGT
LCSGISVGGEVVSVTEYILAPTEVRQYSRDVPFEQERWWAIAAEFQEMSNGKSLLTLKSDARADFNRVVLVDGKALSLKA
STK*					

Nucleotide sequence: 492 bp

						>T6CP000134 NC_003143:549240-549731 [Yersinia pestis CO92] [TssJ]
ATGCGTAGCCTCTTTCGTGTGTTGCCTATTTTTATCCTGACAGGTTGCGTCCTGCTCAGCGGCTGTAGCTGGTTTTCCTC
ATCCTCAGAAGCGGCGCTACAGAAACGGCGGATTGACTTGCAGGTTGTTGCGGCTCCTCTCATCAATCCTGGGCCCAACG
GTCAGGCACAGCCCTTGAAGGTATGCATTATTGAGCTGAATAAAGAGGGGTGGTCACCCCCCGGTTTGTATCAGGGGACG
CTCTGCAGTGGCATCAGTGTAGGAGGTGAAGTCGTCAGCGTGACGGAATACATCCTTGCGCCGACAGAGGTGCGTCAATA
TTCCCGCGATGTTCCCTTTGAACAAGAGCGCTGGTGGGCAATAGCGGCTGAGTTTCAGGAGATGAGTAACGGTAAAAGCC
TATTAACGCTGAAATCTGATGCCCGTGCGGACTTCAACCGTGTGGTGCTCGTTGATGGTAAGGCCCTGTCATTAAAAGCG
TCAACAAAGTAG					