SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000131
Component protein type	TssG
T6SS ID (Type)	T6SS00017 (Type i3)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (328 a.a.); Nucleotide sequence (984 bp)
Reference	[1] Robinson JB, Telepnev MV, Zudina IV, et al. Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models. Microb Pathog. 2009 Nov;47(5):243-51. doi: 10.1016/j.micpath.2009.08.005. Epub 2009 Aug 27. PMID: 19716410 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Cathelyn JS, Crosby SD, Lathem WW, et al. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. Epub 2006 Aug 28. PMID: 16938880

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS03530
Coordinate (Strand)	538864..539847 (+)
NCBI ID	WP_002210476.1
RefSeq	NC_003143
Uniprot ID	Q66EP6_YERPS, A0A0E1NXN6_YERPA, A0A0H2YDR4_YERPN, A0A380PKE1_YERPE, A0A3G5KET5_YERPU
KEGG ID	ype:YPO0505, ypn:YPN_0380, ypu:BZ21_4028, ypj:CH55_2247, ypa:YPA_3591, ypw:CH59_1356, ypq:DJ40_1723, ypr:BZ20_1449, ypl:CH46_409, ypo:BZ17_1911, ypv:BZ15_3066, yps:YPTB0646
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssG	PF06996.13	2.1e-61	19..312

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-327	MASLQTLKNTFLPGISASQDFFELLRRIERATPEQGRLGTQRDRSHLRLRIIQPADMGFAPREVSDIRQTLNTHQHLAEITIFCRHFGLFAPYGPLPIHVTEHARNEALAKRNQAFEHFAGILSQRMAILHYRSWAQMHVAVGHDRASANPFMNHVRQIAGLTPQQALNSHVDRVRRCFPGVYLPGRGSLRKLQEILSLYFSVPIRVEARKGLWIDDSRNIESQRMGRLGNTRIGSRFFDVQHSLVLSIGPVSDPQYLDFQRNSKRINTLVQICHDFVRHRMVLDVQLIIQTSPNMACRLGGGTLSRHSWLKPGSALSIQPIYRTVT

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9852	-	-

Protein sequence: 328 a.a.

						>T6CP000131 NC_003143:538864-539847 [Yersinia pestis CO92] [TssG]
MASLQTLKNTFLPGISASQDFFELLRRIERATPEQGRLGTQRDRSHLRLRIIQPADMGFAPREVSDIRQTLNTHQHLAEI
TIFCRHFGLFAPYGPLPIHVTEHARNEALAKRNQAFEHFAGILSQRMAILHYRSWAQMHVAVGHDRASANPFMNHVRQIA
GLTPQQALNSHVDRVRRCFPGVYLPGRGSLRKLQEILSLYFSVPIRVEARKGLWIDDSRNIESQRMGRLGNTRIGSRFFD
VQHSLVLSIGPVSDPQYLDFQRNSKRINTLVQICHDFVRHRMVLDVQLIIQTSPNMACRLGGGTLSRHSWLKPGSALSIQ
PIYRTVT*					

Nucleotide sequence: 984 bp

						>T6CP000131 NC_003143:538864-539847 [Yersinia pestis CO92] [TssG]
ATGGCTAGCCTCCAGACGCTGAAAAACACGTTTCTGCCGGGTATCTCGGCCAGTCAGGACTTTTTCGAGTTGCTGCGTCG
CATTGAGCGTGCCACTCCAGAGCAAGGCCGGCTTGGCACCCAACGTGATCGTAGCCATCTGCGGCTACGTATTATTCAGC
CTGCCGATATGGGATTTGCACCGCGTGAAGTGAGTGATATCAGGCAAACGCTGAACACTCATCAGCATCTGGCAGAGATC
ACCATTTTCTGCCGCCATTTTGGTTTATTTGCCCCGTATGGCCCGTTGCCAATACATGTTACCGAACATGCTCGTAACGA
AGCGTTGGCTAAACGTAATCAGGCCTTTGAACATTTTGCGGGCATTCTCAGCCAGCGAATGGCGATCTTGCATTATCGCT
CTTGGGCCCAAATGCACGTCGCCGTGGGGCATGATCGCGCGTCAGCCAATCCCTTTATGAACCATGTGCGTCAAATCGCG
GGCCTAACGCCGCAGCAAGCGCTGAATTCACATGTGGATCGGGTACGGCGTTGCTTTCCGGGGGTGTATCTGCCGGGCAG
AGGATCATTACGCAAATTACAGGAGATCCTGAGCCTGTATTTCTCGGTTCCTATCCGTGTTGAAGCCCGTAAAGGATTGT
GGATTGACGACTCGCGCAATATAGAAAGCCAACGTATGGGACGGTTGGGTAACACGCGTATTGGCAGCCGTTTCTTCGAT
GTGCAGCACAGCCTGGTGTTATCTATTGGTCCGGTATCTGACCCCCAATATCTGGATTTTCAACGCAACAGCAAACGCAT
TAACACTCTGGTGCAAATCTGCCATGACTTTGTCCGCCATCGGATGGTGCTGGACGTGCAATTAATCATACAGACCTCAC
CCAATATGGCTTGTCGTCTTGGCGGTGGAACGCTCAGTCGCCATAGCTGGTTAAAGCCAGGCTCAGCCTTGAGTATACAG
CCAATTTATAGAACGGTTACCTAG					