SecReT6, T6SS database

Detailed information of component protein

Summary

Component ID	T6CP000130
Component protein type	TssF
T6SS ID (Type)	T6SS00017 (Type i3)
Strain	Yersinia pestis CO92
Replicon	chromosome
Sequence	Protein sequence (615 a.a.); Nucleotide sequence (1845 bp)
Reference	[1] Robinson JB, Telepnev MV, Zudina IV, et al. Evaluation of a Yersinia pestis mutant impaired in a thermoregulated type VI-like secretion system in flea, macrophage and murine models. Microb Pathog. 2009 Nov;47(5):243-51. doi: 10.1016/j.micpath.2009.08.005. Epub 2009 Aug 27. PMID: 19716410 [2] Boyer F, Fichant G, Berthod J, et al. Dissecting the bacterial type VI secretion system by a genome wide in silico analysis: what can be learned from available microbial genomic resources? BMC Genomics. 2009 Mar 12;10:104. doi: 10.1186/1471-2164-10-104. PMID: 19284603 [3] Cathelyn JS, Crosby SD, Lathem WW, et al. RovA, a global regulator of Yersinia pestis, specifically required for bubonic plague. Proc Natl Acad Sci U S A. 2006 Sep 5;103(36):13514-9. Epub 2006 Aug 28. PMID: 16938880

Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene)	YPO_RS03525
Coordinate (Strand)	537027..538871 (+)
NCBI ID	WP_002210477.1
RefSeq	NC_003143
Uniprot ID	A0A0E1NR95_YERPA, A0A0H2YFH3_YERPN, A0A5P8YJJ8_YERPE
KEGG ID	ypn:YPN_0379, ype:YPO0504, ypa:YPA_3592
PDB ID	-

Pfam domain hit(s)

Domain	Pfam ID	E-value	Aligned region
T6SS_TssF	PF05947.14	9e-123	4..609

Transmembrane helices

Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction	Region	Sequence
Outside	1-614	MPNESFLALYNEELRYLREDGQRFAHMHPQVAQHLGLHADGVLDPFVERLLEGTAFLSSRIQQRLNNEHPEFALQMLGRLAPLWYTPLPSIATIAMVPDLTSPQWHSRVVLPKGSRVTLSDSSLNNRLANFSTGRTLKIQPLVIENAECASTPPAYLPLSVASQLQAGQAHLRLRLTTQGVASLSELDFAPMNLTLAGGIIQSNQLITTLLNNTLHIVLWANTDGKPLVKVLTKEHLRQGGLDEQEALLPVAISEMPGNRLLREYFAAPSRFFDLELHGLNDFLQQGDGIHTFEVLFVLDQRPLHLIDRVTGNDFRLFATPIINLFKRRCSPVLINGEHTEYPVIVDPLNPRLYEVHHVLGVNGLLSEGGSVQFSSLHGHARFDDDDKMAGYSIRRRRDFTDKNHQKNSPLPHDSLFIALSPGTSGIDIDHVKSLSIEAMVCERHLIPSQLQHPEFKLEIALPVGQIEMLRHPAKPQGVPNIDQAWRALQLIANNPLRYALPDVTDCSPLLKDWLSIFCQHEEASQHKRITSLTHASVAHNFERHTAPGPIAWSRGVEATLDFNSSHHSDKGAFLFARILHHALSEYCELGQTLRLHMQVDGETFAKWGPVNYG

Signal peptides

Signal peptides and corresponding cleavage sites are predicted using SignalP 5.0 software.

Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).

Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).

Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).

Prediction	Probability	Cleavage site	Signal peptide sequence
Other	0.9961	-	-

Protein sequence: 615 a.a.

						>T6CP000130 NC_003143:537027-538871 [Yersinia pestis CO92] [TssF]
MPNESFLALYNEELRYLREDGQRFAHMHPQVAQHLGLHADGVLDPFVERLLEGTAFLSSRIQQRLNNEHPEFALQMLGRL
APLWYTPLPSIATIAMVPDLTSPQWHSRVVLPKGSRVTLSDSSLNNRLANFSTGRTLKIQPLVIENAECASTPPAYLPLS
VASQLQAGQAHLRLRLTTQGVASLSELDFAPMNLTLAGGIIQSNQLITTLLNNTLHIVLWANTDGKPLVKVLTKEHLRQG
GLDEQEALLPVAISEMPGNRLLREYFAAPSRFFDLELHGLNDFLQQGDGIHTFEVLFVLDQRPLHLIDRVTGNDFRLFAT
PIINLFKRRCSPVLINGEHTEYPVIVDPLNPRLYEVHHVLGVNGLLSEGGSVQFSSLHGHARFDDDDKMAGYSIRRRRDF
TDKNHQKNSPLPHDSLFIALSPGTSGIDIDHVKSLSIEAMVCERHLIPSQLQHPEFKLEIALPVGQIEMLRHPAKPQGVP
NIDQAWRALQLIANNPLRYALPDVTDCSPLLKDWLSIFCQHEEASQHKRITSLTHASVAHNFERHTAPGPIAWSRGVEAT
LDFNSSHHSDKGAFLFARILHHALSEYCELGQTLRLHMQVDGETFAKWGPVNYG*					

Nucleotide sequence: 1845 bp

						>T6CP000130 NC_003143:537027-538871 [Yersinia pestis CO92] [TssF]
ATGCCCAACGAAAGTTTTCTGGCTCTATACAACGAAGAGTTGCGTTATCTGCGTGAAGATGGGCAGCGCTTCGCTCATAT
GCATCCGCAAGTCGCCCAACACCTTGGGTTACATGCTGATGGTGTGCTCGATCCTTTCGTCGAACGGCTCCTTGAAGGCA
CCGCTTTCTTGAGTTCCCGTATCCAACAGCGTTTGAACAACGAGCATCCAGAGTTTGCTTTGCAAATGCTTGGGCGTTTG
GCCCCACTTTGGTATACACCCCTGCCGTCGATTGCCACCATTGCCATGGTTCCGGATCTTACGTCACCGCAATGGCACTC
GCGCGTCGTATTGCCTAAAGGTAGCCGCGTGACATTGAGTGACAGTTCGCTGAACAACCGGTTGGCGAATTTCTCTACTG
GCCGCACGCTGAAAATACAGCCATTGGTCATTGAAAATGCCGAGTGTGCCAGCACGCCACCGGCGTATTTACCGCTCTCT
GTGGCTTCACAATTACAAGCTGGTCAGGCCCATCTGCGCTTGCGTCTGACCACTCAAGGTGTCGCGTCTTTATCTGAGCT
GGATTTCGCCCCGATGAACCTGACGCTAGCGGGGGGAATAATTCAGTCAAATCAGTTAATCACGACACTGTTGAATAACA
CCTTACATATTGTTTTGTGGGCCAACACGGATGGGAAACCGCTGGTCAAAGTTCTTACCAAAGAACATCTTCGCCAGGGA
GGGCTGGATGAACAAGAGGCGCTTTTACCTGTTGCGATCAGCGAAATGCCTGGAAACCGTTTGCTACGAGAATATTTTGC
TGCTCCTTCGCGTTTTTTCGATCTGGAACTGCATGGCCTCAACGACTTCCTGCAACAAGGGGACGGCATTCATACCTTTG
AGGTGCTGTTTGTACTGGATCAGCGCCCCCTACATTTGATTGATAGGGTCACTGGCAATGACTTCCGACTCTTCGCCACA
CCTATTATTAACCTGTTCAAACGCCGTTGTAGCCCGGTGCTGATTAATGGTGAACACACCGAATATCCCGTCATTGTTGA
CCCCCTGAACCCCCGCTTGTACGAAGTCCATCATGTACTCGGGGTCAACGGTTTGTTGAGCGAAGGGGGGAGCGTGCAGT
TTTCTTCCCTTCATGGTCATGCCCGTTTTGATGATGACGATAAAATGGCGGGTTACAGCATTCGCCGTCGCCGCGACTTC
ACGGATAAAAATCATCAGAAAAATTCACCTTTACCTCATGACAGCTTATTCATTGCCCTTTCACCGGGGACCAGCGGGAT
AGATATTGATCACGTCAAGTCGCTGTCGATTGAAGCCATGGTGTGCGAACGCCACTTAATTCCCAGCCAATTACAGCATC
CGGAATTCAAGCTGGAAATTGCCCTGCCAGTGGGGCAGATCGAAATGCTGCGTCACCCGGCAAAACCACAGGGTGTGCCG
AATATTGATCAAGCCTGGCGGGCGTTACAACTGATTGCCAACAATCCGCTGCGTTATGCGCTGCCGGATGTCACCGATTG
TTCGCCGCTGCTAAAAGATTGGCTGTCGATATTTTGTCAGCATGAAGAGGCGAGCCAGCATAAGAGGATCACTAGCCTGA
CTCACGCCAGTGTGGCGCATAATTTTGAACGTCATACCGCTCCGGGGCCGATCGCCTGGAGCCGGGGGGTTGAAGCCACA
CTGGATTTCAACAGCAGCCACCACAGTGATAAAGGTGCATTCCTCTTTGCGCGGATCCTGCATCACGCGCTGTCAGAATA
TTGTGAATTAGGGCAGACCCTGAGGTTACACATGCAGGTTGATGGTGAAACCTTCGCCAAATGGGGGCCTGTCAACTATG
GCTAG					