Detailed information of component protein


Summary

Component ID T6CP000091
Component protein type TssJ
T6SS ID (Type) T6SS00013 experimental (Type i1)
Strain Pseudomonas putida KT2440
Replicon chromosome
Sequence Protein sequence (266 a.a.); Nucleotide sequence (798 bp)
Reference
experimental Experimental investigation has been performed on this T6SS

External database links

Locus tag (Gene) PP_RS13650
Coordinate (Strand) 2994467..2995264 (-)
NCBI ID WP_010953538.1
RefSeq NC_002947
Uniprot ID Q88JN1_PSEPK, A0A2V4FAN5_9PSED
KEGG ID ppu:PP_2618
PDB ID -

Pfam domain hit(s)

Domain Pfam ID E-value Aligned region
T6SS-SciN PF12790.9 3.1e-19 157..255

Transmembrane helices

  • Transmembrane helices are predicted using TMHMM 2.0 software.

Prediction                 Region     Sequence
Outside 1-265 MKPHPLLLASLLLALVAPTGCTSLSRMSQVAMDHSLPIGPPKDHPTEVAFSINASPTLNGNPDSLDVAADQDRALEPSPYAVTLSAGDPYALTEKIGVLFEYLQAQFPAMSPVELDEGDESDPARSPLEESGPGSYDDPQVALTLPHTTSVASALVATPIAITILQLRDDSLLRNTVYQLLEQDPAKALRSTYIRSDDYLLRPGQFKYIPFEPIHAETRFVAVIADYRGHENASWRQVLRIPPRGRQIMLSVLVNDTHIVLKEED



Signal peptides
  • Sec/SPI: "standard" secretory signal peptides transported by the Sec translocon and cleaved by Signal Peptidase I (Lep).
  • Sec/SPII: lipoprotein signal peptides transported by the Sec translocon and cleaved by Signal Peptidase II (Lsp).
  • Tat/SPI: Tat signal peptides transported by the Tat translocon and cleaved by Signal Peptidase I (Lep).
Prediction        Probability Cleavage site Signal peptide sequence
LIPO(Sec/SPII) 0.9896 pos: 20-21. PTG-CT. MKPHPLLLASLLLALVAPTG



  Protein sequence: 266 a.a.    

>T6CP000091 NC_002947:c2995264-2994467 [Pseudomonas putida KT2440] [TssJ]
MKPHPLLLASLLLALVAPTGCTSLSRMSQVAMDHSLPIGPPKDHPTEVAFSINASPTLNGNPDSLDVAADQDRALEPSPY
AVTLSAGDPYALTEKIGVLFEYLQAQFPAMSPVELDEGDESDPARSPLEESGPGSYDDPQVALTLPHTTSVASALVATPI
AITILQLRDDSLLRNTVYQLLEQDPAKALRSTYIRSDDYLLRPGQFKYIPFEPIHAETRFVAVIADYRGHENASWRQVLR
IPPRGRQIMLSVLVNDTHIVLKEED*

  Nucleotide sequence: 798 bp    

>T6CP000091 NC_002947:c2995264-2994467 [Pseudomonas putida KT2440] [TssJ]
ATGAAACCTCATCCACTTCTGCTCGCCTCCCTGCTGCTTGCGCTGGTCGCGCCCACCGGCTGCACCTCTTTGAGCAGAAT
GAGCCAGGTGGCCATGGACCACTCGTTGCCCATCGGGCCGCCCAAGGATCACCCGACCGAGGTCGCATTCAGCATCAATG
CCAGCCCTACCCTCAACGGCAATCCGGACAGCCTTGACGTCGCAGCCGATCAGGATCGCGCCCTGGAGCCCAGCCCCTAC
GCTGTGACCCTGAGTGCTGGCGACCCCTATGCGTTGACCGAAAAAATCGGGGTTCTGTTCGAATACCTACAAGCGCAATT
CCCGGCCATGTCCCCCGTCGAGCTGGACGAAGGAGACGAAAGCGACCCGGCTCGCTCGCCCCTGGAAGAAAGCGGCCCCG
GCAGCTACGACGACCCTCAGGTGGCGTTGACCTTGCCGCACACCACAAGCGTTGCCTCAGCGCTGGTCGCCACGCCCATT
GCCATCACGATCCTGCAACTGCGCGACGACTCACTGCTGCGCAACACCGTGTACCAGCTACTGGAGCAGGACCCGGCCAA
GGCGCTGCGCAGTACCTATATCCGTAGTGACGACTACCTGCTGCGCCCTGGCCAATTCAAGTACATCCCGTTCGAACCCA
TCCACGCCGAAACCCGCTTCGTCGCGGTGATCGCCGACTACCGAGGCCATGAAAACGCCAGTTGGCGGCAAGTACTGCGC
ATTCCGCCGCGTGGCCGCCAGATCATGCTGTCGGTGTTGGTCAACGACACGCACATCGTGCTCAAGGAAGAAGACTGA